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Product Info Summary
| SKU: | A03118-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Bub3 Antibody
SKU/Catalog Number
A03118-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Bub3 Antibody catalog # A03118-1. Tested in WB,ICC,IF,IP,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bub3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03118-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein corresponding to the N-terminus of human Bub3.
Reactive Species
A03118-1 is reactive to BUB3 in Human, Mouse, Rat
Calculated molecular weight
37.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03118-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution. Positive control: Lane 1: A549 Lane 2: HL-60 Lane 3: A431 Lane 4: Rat colon
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ICC staining Bub3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Specific Publications For Anti-Bub3 Antibody (A03118-1)
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