BUB3 Antibodies

Mitotic checkpoint protein BUB3 (BUB3)

Has a double role in spindle-assembly checkpoint signaling and in promoting the establishment of proper kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments.

Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis.

Gene Information

Human
Gene Name:	BUB3
Uniprot:	O43684
Entrez:	9184

Family

Belongs to:
WD repeat BUB3 family

Alternative Names

BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog; Bub3; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog (yeast); hBUB3; mitotic checkpoint component; mitotic checkpoint protein BUB3

Molecular Weight

Mass (kDA):
37.155 kDA

Genomic Context

Human
Location:	10q26.13
Sequence:	10; NC_000010.11 (123154244..123170467)

Subcellular Localizations

Nucleus. Chromosome, centromere, kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.

Diseases

• Malignant Neoplasms
• Neoplasms
• Aneuploidy
• Carcinoma
• Cell Transformation, Neoplastic
• Instabilities, Chromosomal
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Genomic Instability
• Polyploidy
• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Lung
• Cytogenetic Abnormality

• Carcinogenesis
• Leukemia
• Liver Carcinoma
• Tetraploidy
• Malignant Tumor Of Colon
• Liver Neoplasms
• Melanoma

Interacting Proteins

• BUB1
• BUB1B
• PSTPIP1

Pathways

• Anaphase
• Spindle Checkpoint
• Mitosis
• Spindle Assembly
• Spindle Assembly Checkpoint
• Cell Cycle
• Chromosome Segregation
• Metaphase
• Localization
• Kinetochore Localization
• Cell Division
• Interphase
• Meiosis

• Cell Cycle Arrest
• Dna Replication
• Proteolysis
• Cytokinesis
• Prophase
• Dna Repair
• Transport

PTMs

• Phosphorylation
• Ubiquitination

• Cleavage
• Dephosphorylation

• Methylation

Research Areas

• Cell Cycle and Replication
• Core ESC-Like Genes
• DNA Repair
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BUB3

Best Uses Of The BUB3 Marker

Are you looking to find a high-quality primary antibody for your research? Boster Bio has a wide range of antibodies that you might be interested in. Their antibodies have been validated for use in Western Blotting, Immunohistochemistry, and ELISA. The Boster antibody collection includes high-affinity Antigens and is highly respected by the research community. Read on to learn more about the Boster Bio company and its products.

Boster provides high-affinity primary antibodies

Whether you're looking for a specific protein to study or an antibody to use in ELISA, Boster Bio has the product you need. Their high-affinity antibodies have been validated for multiple assays, including Western Blotting and Immunohistochemistry. In addition to primary antibodies, Boster offers a wide range of secondary antibodies, control reagents, and kits.

The company's high-affinity primary antibodies have been characterized through various means, including differential scanning fluorimetry (DSF). Using a series control sequences of antibodies, mAb5D5 recognized the PfCSPsporozoites with a Tm value 78.8 +/0.4 degC (CR3022). CB6 and VHH72 had Tm values 67.4 +/0.5 degC (VHH-72). However, the IgGs found in this research had Tm values at 72.2 +/0.3degC (12H2). Additionally, the 13I1 DVD showed multiple unfolding transitions that indicate that the antibodies were stable.

Boster Bio provides highly purified recombinant Abs for a variety of applications. In addition to their effectiveness in immunological research, they also provide a wide range of other applications. These high affinity primary antibodies are a great tool for testing new vaccines. They are also very effective in detecting infectious diseases. Boster Bio has a range of high-affinity prima antibodies that will help you build a strong and effective immune system.

One example is the VHH72 anti SARS virus virus. This antibody has a high affinity against the virus. These antibodies also have limited neutralizing activity, suggesting that vaccines may produce superior immune responses. The VHH72 anti SARS-CoV-2 antibody IC50 was 2.32 +/ 0.27 nM. These are good signs for developing COVID-19 vaccines.

Historie

The BUB3 marker is located on chromosome 10,q24-q26. This gene regulates DMAP1 mediated DNA methylation. The resistance to mitotic stresses in pancreatic cancer is also linked with the BUB3 genes. Several hypotheses have been proposed to explain the presence of BUB3 in this gene. These hypotheses are further discussed in this article.

This gene encodes a proteinkinase which phosphorylates BUB3 in kinetochores. BUB1 in yeast is found at kinetochores prior to chromosome alignment. Taylor et al. (1993). Taylor et.al.

BubR1 interacts with Bub3 through cooperative recognition. The C-terminal portion of the peptide may require Bub3 loop regions to undergo conformational changes. The interaction involves important contacts between Bub3R1 and Bub3. This makes the enthalpy contributions relatively high. Additional contacts are required near the peptide to prevent a low-affinity interaction. The short-lived interaction between BubR1 and Bub3 suggests that a complex-forming interaction requires important contacts between the proteins.

A disruption of the BubR1–Bub3 interaction is another possible mechanism to block SAC signaling. Inhibition of SAC signaling in human cells may involve disrupting this interaction. This interaction is vital for normal SAC Signaling. BubR1 & Bub3 are essential for the SAC pathway. A potential treatment strategy is to disable Bub3-BubR1 interactions.

Current projects

It is still not clear what role the BUB3 marker plays in cancer. It has been shown that expression of the BUB3 marker in human sarcoma cells and tissues is associated to a lower overall survival rate and disease-free survival. This research may provide answers to the question "What are your best treatments for sarcomas?"

Recent research has revealed that BUBs play an important role in immune cell biology, including regulation of the cell cycle. Several of these studies are currently being conducted to determine how these proteins contribute to a healthy immune system. BUBs also play a role in the infection of human T-lymphotropic viruses. Researchers are currently studying the role of BUBs within the cell cycle. This research was funded by the National Institutes of Health, Tianjin Municipal Health Commission, and other funding agencies.

The BUB3 protein family is made up of a number proteins that participate in many body processes. The BUB3 protein is a member of this family. It binds its partner protein BUB1. Both proteins recognize eachother and work together during Mitosis. Many of these proteins play a role in controlling the cell cycle, including BUB1/BUB3 and BUB1/BUB3. BUB3 has also been found in many types of cancer, including oral. BUB3 inhibition could increase the chemosensitivity in tumors.

Bub3 plays a crucial role in regulating microtubule interaction during chromosome differentiation. Monitoring chromosome segregation is possible when microtubules are depolymerized and MBC is washed away. The presence of Bub3 is required for proper kinetochore-microtubule attachment. Bub3-deficient cells are less likely to achieve bi-orientation.

BUB3 is an extremely low-level protein. However, it is essential to the mitotic process. BUB3 could not be visually scored in the previous study because of technical difficulties. The accuracy of BUB3 counts depends on how many tumor cells are clustered. This study may help us to better understand tumor morphology. To prove its utility, however we need to validate BUB3 in modern patient cohorts.

Future plans

The BUB3 genes is an essential regulator of cell-cycle progression. Low-grade luminal breast carcinoma is associated with BUB3 gene expression, and colorectal cancer is associated with mutations in the gene. Overexpression of the BUB3 marker has been shown to be associated with poor prognosis in breast carcinoma. Future research will be conducted on the marker's role in cancer progression. Researchers have already started to use it for cancer diagnostics.

The N-terminal portion of the Bub3 protein interacts tightly with the peptide. This interaction is thought to contribute to the overall binding affinity of the protein. Mutating the GLEBS domain can also be used to determine the binding kinetic parameters for the BUB3 protein. A Glu-394 salt bridge is found in the N-terminal and amino acids 400-419 are also present. The second-smallest koff is found in the mutant BUBR1392-425, E394K.

The mitotic control point complex includes the BUB3 genes. It is a member of the mitotic checkpoint complex and works with other proteins to prevent premature onset. Mutation of BUB3 in knockout mice causes tumorigenesis. Knockout mice exhibit high proliferative capacity and aneuploidy. Numerous studies in humans have linked BUB3 gene mutations to various types of cancer, including colorectal. BUB3 genes are expressed in different places in colorectal tumors. The highest levels of expression are found in CLP.