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Product Info Summary
| SKU: | A07686 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CARD 10 Antibody
SKU/Catalog Number
A07686
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CARD 10 Antibody catalog # A07686. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CARD 10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07686)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CARD10. AA range:481-530
Reactive Species
A07686 is reactive to CARD10 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
115.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07686 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using CARD 10 Polyclonal antibody.
Specific Publications For Anti-CARD 10 Antibody (A07686)
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Customer Q&As
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5 Customer Q&As for Anti-CARD 10 Antibody
Question
We are currently using anti-CARD 10 antibody A07686 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
The anti-CARD 10 antibody (A07686) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-14
Question
Does anti-CARD 10 antibody A07686 work on horse WB with parotid gland?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
Our lab technicians have not validated anti-CARD 10 antibody A07686 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CARD 10 antibody A07686 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse parotid gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-29
Question
Does A07686 anti-CARD 10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Li
Verified customer
Asked: 2015-05-13
Answer
As indicated on the product datasheet, A07686 anti-CARD 10 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-05-13
Question
I was wanting to use your anti-CARD 10 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
S. Dhar
Verified customer
Asked: 2013-12-26
Answer
It shows on the product datasheet, A07686 anti-CARD 10 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-12-26
Question
I see that the anti-CARD 10 antibody A07686 works with WB, what is the protocol used to produce the result images on the product page?
T. Taylor
Verified customer
Asked: 2013-08-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-08-06
