Anti-CARMA3 CARD10 Antibody

Product Name

Anti-CARMA3 CARD10 Antibody

View all CARD10 Antibodies

SKU/Catalog Number

A07686-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CARMA3 CARD10 Antibody (Catalog # A07686-2). Tested in ELISA, IHC-P applications. This antibody reacts with Human.

Storage & Handling

CARMA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CARMA3 CARD10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07686-2)

Host

Rabbit

Contents

CARMA3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CARMA3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CARMA3. The immunogen is located within the first 50 amino acids of CARMA3.

Cross-reactivity

CARMA3 antibody is human specific. At least three isoforms of CARMA3 are known to exist; this antibody will only detect isoform 1. CARMA3 antibody is predicted not to cross-react with other CARMA proteins.

Reactive Species

A07686-2 is reactive to CARD10 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

115.9 kDa

Background of CARD10

CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. As with CARMA1, the CARD domain of CARMA3 has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-κB activation. When expressed in cells, this protein binds to BCL10 and activates NF-κB Recent experiments have shown that CARMA3 is required for EGF-induced NF-κB activation and contributes to tumor growth in vivo, suggesting that CARMA3 may serve as a new therapeutic target for the treatment of EGFR-driven tumors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07686-2 is guaranteed for ELISA, IHC-P Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CARMA3 antibody can be used for detection of CARMA3 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CARD10

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