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Product Info Summary
| SKU: | A03404 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-CARD8 (Tucan) Antibody
SKU/Catalog Number
A03404
Size
50 µl
Form
Liquid
Description
Boster Bio Anti-CARD8 (Tucan) Antibody (Catalog# A03404). Tested in WB, IHC, IP application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-CARD8 (Tucan) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03404)
Host
Rabbit
Contents
50 μl sera
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Full-length recombinant protein of human TUCAN (CARD8)
Reactive Species
A03404 is reactive to CARD8 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
48.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03404 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC (paraffin): 1:1000-1:5000
IHC (frozen): Users should optimize
IP: 1:50-1:200
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded tumor/normal adjacent tissue cores from a human colon tissue microarray stained for TUCAN/CARD8 expression at 1:2000. A and B are tumor tissue cores. A1 and B1 are the matched normal adjacent cores from A and B, respectively. Hematoxylin-eosin counterstain.
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Formalin-fixed, paraffin-embedded tissue human colon microarray stained for TUCAN/CARD8 expression at 1:2000. A-A2, sucessively higher magnifications of the microarray. A, low magnification overview. A1, differential TUCAN/CARD8 staining between tissue cores is observed. A2, Areas of intense TUCAN/CARD8 staining in a colon carcinoma tissue core. Hematoxylin eosin counterstain.
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Western blot analysis of TUCAN/CARD8 in tumor cell lines using primary antibody dilution ratio of 1:2000. Cell lysates were normalized for total protein content.
Specific Publications For Anti-CARD8 (Tucan) Antibody (A03404)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-CARD8 (Tucan) Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical cord blood using anti-CARD8 (Tucan) antibody A03404. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-14
Question
I have attached the WB image, lot number and protocol we used for umbilical cord blood using anti-CARD8 (Tucan) antibody A03404. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-02
Question
We are currently using anti-CARD8 (Tucan) antibody A03404 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
D. Jones
Verified customer
Asked: 2017-04-11
Answer
The anti-CARD8 (Tucan) antibody (A03404) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-04-11
