Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250

Boster Bio Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 catalog # M00334-8. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 12 publication(s).

Product Info Summary

SKU: M00334-8
Size: 100ul
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250

See all Caspase-3 products

SKU/Catalog Number

M00334-8

Size

100ul

Description

Boster Bio Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 catalog # M00334-8. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-8)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM250

Immunogen

A synthetic peptide corresponding to residues within human Caspase-3 subunit p17

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00334-8 is reactive to CASP3 in Human

Applications

M00334-8 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:1000-1:2500 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For CASP3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P42574

Gene ID

836

Gene Name

CASP3

Full Name

Caspase-3

Weight

31.608kDa

Superfamily

peptidase C14A family

Alternative Names

Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CASP3, check out the CASP3 Infographic

CASP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CASP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00334-8 has been cited in 12 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Maternal separation exacerbates Alzheimer's disease-like behavioral and pathological changes in adult APPswe/PS1dE9 mice

MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/%u03B2-catenin signaling

In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

Enhanced anti-apoptosis and gut epithelium protection function of acidic fibroblast growth factor after cancelling of its mitogenic activity

Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction

An J, Liu T, She R, Wu Q, Tian J, Shi R, Hao W, Ren X, Yang Y, Lu Y, Yang Y, Wu Y. Oncotarget. 2017 Dec 17;9(4):4475-4484. doi: 10.18632/oncotarget.23381. eCollection 2018 Jan 12. Replication of hepatitis E virus in the ovary and promotion of oocy...

Liu Nb, Peng T, Pan C, Yao Yy, Shen B, Leng J. World J Gastroenterol. 2005 Oct 28;11(40):6281-7. Overexpression Of Cyclooxygenase-2 In Human Hepg2, Bel-7402 And Smmc-7721 Hepatoma Cell Lines And Mechanism Of Cyclooxygenase-2 Selective Inhibitor Ce...

Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.

Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L. Neural Regen Res. 2014 Apr 15;9(8):837-44. Doi: 10.4103/1673-5374.131600. Apoptosis Is An Obstacle To The Differentiation Of Adipose-Derived Stromal Cells Into Astrocytes.

Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...

Chu Sh, Ma Yb, Feng Df, Zhang H, Qiu Jh, Zhu Za. Oncol Lett. 2011 Nov;2(6):1139-1142. Epub 2011 Aug 4. Elevated Expression Of Solute Carrier Family 22 Member 18 Increases The Sensitivity Of U251 Glioma Cells To Bcnu.

Tian W, Wei T, Li B, Wang Z, Zhang N, Xie G. Plos One. 2014 May 6;9(5):E96775. Doi: 10.1371/Journal.Pone.0096775. Ecollection 2014. Pathway Of Programmed Cell Death And Oxidative Stress Induced By ??-Hydroxybutyrate In Dairy Cow Abomasum Smooth Mu...

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16 Customer Q&As for Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250

Question

We want to test anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

The products we sell, including anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-11

Question

We have tried in the past anti-Caspase-3 Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

I have checked the website and datasheets of our anti-Caspase-3 Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2020-02-07

Question

Is a blocking peptide available for product anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 (M00334-8)?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

We do provide the blocking peptide for product anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 (M00334-8). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-03

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-23

Question

My boss were happy with the WB result of your anti-Caspase-3 Rabbit Monoclonal antibody, Clone

S. Carter

Verified customer

Asked: 2019-12-09

Answer

Based on literature, cervix carcinoma erythroleukemia does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-12-09

Question

Does anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 work for IHC with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

According to the expression profile of cervix carcinoma erythroleukemia, CASP3 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 will work for IHC with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2019-12-06

Question

Is there a BSA free version of anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 available?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-28

Question

Will M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

You can see on the product datasheet, M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-23

Question

We have been able to see staining in human tongue. What should we do? Is anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 supposed to stain tongue positively?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

From what I have seen in literature tongue does express CASP3. From what I have seen in Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-10-15

Question

I see that the anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-23

Question

I have a question about product M00334-8, anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-18

Question

I was wanting to use your anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 for IHC for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2017-10-23

Answer

You can see on the product datasheet, M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-23

Question

I have attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-07-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-03

Question

Is this M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 reactive to the isotypes of CASP3?

Verified Customer

Verified customer

Asked: 2017-06-02

Answer

The immunogen of M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 is A synthetic peptide corresponding to residues within human Caspase-3 subunit p17. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-06-02

Question

We are currently using anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?

L. Taylor

Verified customer

Asked: 2016-03-04

Answer

The anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 (M00334-8) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-03-04

Question

I am looking for using your anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 for luteolysis studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.

B. Miller

Verified customer

Asked: 2014-03-05

Answer

I appreciate your inquiry. This M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 is tested on jurkat cell lysate, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-03-05

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M00334-8
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$382.00

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