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- Table of Contents
Casp9 antibody (A00080-4) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of mouse spleen tissues using anti-Caspase-9 p35/Casp9 antibody (A00080-4).
Overlay histogram showing mouse spleen tissues stained with A00080-4 (Blue line). To facilitate intracellular staining, tissues were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9 p35/Casp9 Antibody (A00080-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing mouse spleen tissues stained with A00080-4 (Blue line). To facilitate intracellular staining, tissues were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9 p35/Casp9 Antibody (A00080-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Caspase-9 p35/Casp9 Antibody Picoband®
Casp9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
Casp9 antibody was positively detected by WB in:
mouse heart tissue, rat heart tissue, rat skeletal muscle tissue,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Casp9 antibody was positively detected by FC in:
mouse spleen tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
FC
Mouse
Tested
Recommend
Tested
Rat
Tested
Recommend
Product Information
Product Name
Anti-Caspase-9 p35/Casp9 Antibody Picoband®
View all CASP9 Antibodies
View all CASP9 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Caspase-9 p35/Casp9 recombinant protein (Position: E3-D266).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37/46 kDa
Calculated Molecular Weight
50.0 kDa
Background of Casp9
Caspase-9 is an enzyme that in humans is encoded by the CASP9 gene. This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Cite This Product
Anti-Caspase-9 p35/Casp9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00080-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
89%
Moderate Homology
Sheep
72%
Moderate Homology
Cow
66%
Low homology
Dog
66%
Low homology
Human
63%
Low homology
Monkey
63%
Low homology
Rabbit
58%
Low homology
Zebrafish
39%
Low homology
Drosophila
18%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Casp9 antibody
Flow Cytometry (FC) protocol for Casp9 antibody
Publications
Application:
Species:
Structural characteristics and anti-tumor effect of low molecular weight Dendrobium officinale polysaccharides by reconstructing tumor microenvironment
Published: 2024-06-20
Journal: Journal of Functional Foods
Application: WB
Impact Factor: 3.80
Species: Mouse
Regulatory role of oxidative stress in retrorsine – Induced apoptosis and autophagy in primary rat hepatocytes
Published: 2024-05-28
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 6.20
Species: Rat
PMID: 38810283
Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology
Published: 2021-05-06
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.81
Species: Rat
PMID: 34025396
Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway
Published: 2021-02-22
Journal: Drug Design Development and Therapy
Application: WB
Impact Factor: 4.16
Species: Human
PMID: 33654381
Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice
Published: 2019-09-01
Journal: Antioxidants
Application: WB
Impact Factor: 4.52
Species: Mouse
PMID: 31480577
Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration
Published: 2019-04-08
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 3.45
Species: Mouse
PMID: 30974116
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Product Promise
We promise Anti-Caspase-9 p35/Casp9 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00080-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026