Anti-Caspase 9/CASP9 Antibody

Caspase-9 antibody

Boster Bio Anti-Caspase 9/CASP9 Antibody catalog # PA1595. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PA1595
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Caspase 9/CASP9 Antibody

View all Caspase-9 Antibodies

SKU/Catalog Number

PA1595

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase 9/CASP9 Antibody catalog # PA1595. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase 9/CASP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1595)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Caspase-9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1595 is reactive to CASP9 in Human

Applications

PA1595 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

46.281kDa

Background of Caspase-9

CASP9 (CASPASE9), also called APAF3, is an initiator caspase, encoded by the CASP9 gene. The CASP9 gene is mapped to chromosome 1p36.3-p36.1 by FISH. CASP9 is identified as a member of the caspase family that participates in caspase-3 activation in vitro. And it also regarded as the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Its genomic coordinates (GRCh37) is 1:15,818,768-15,851,284. The crystal structure of CASP9 is complex with the BIR3 in an inhibitory domain of XIAP at 2.4-angstrom resolution and the CASP9 gene contains 9 exons and spans approximately 35 kb of genomic DNA. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome c and dATP, an event that leads to caspase-9 activation. CASP9 activity increases dramatically upon association with the apoptosome complex. And the majority of Casp9 knockout mice died perinatally with a markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CASP9 (Source: Uniprot.org, NCBI)

Gene Name

CASP9

Full Name

Caspase-9

Weight

46.281kDa

Superfamily

peptidase C14A family

Alternative Names

APAF-3APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase9; Caspase-9; EC 3.4.22.62; ICE-LAP6CASPASE-9c; ICE-like apoptotic protease 6; MCH6apoptosis-related cysteine protease CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 caspase 9 caspase-9|ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9, apoptosis-related cysteine peptidase|protein phosphatase 1, regulatory subunit 56

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CASP9, check out the CASP9 Infographic

CASP9 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CASP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1595 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li QY,Hou CZ,Yang LP,Chu XL,Wang Y,Zhang P,Zhao Y. Study on the Mechanism of Ginseng in the Treatment of Lung Adenocarcinoma Based on Network Pharmacology. Evid Based Complement Alternat Med.2020 Jul 31;2020:2658795. doi:10.1155/2020/2658795.PMID:32802118
Species: Human
PA1595 usage in article: APP:ICC, SAMPLE:A549 CELL, DILUTION:NA

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells

In vivo?and?in vitro?evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer

Shi, C., Ma, Q., Ren, M., Liang, D., Yu, Q., & Luo, J. (2017). Antitumorpharmacological mechanism of the oral liquid of Poriacocos polysaccharide. Journal of Ethnopharmacology. Advance online publication. doi: 10.1016/j.jep.2017.07.003

Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.

Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L. Neural Regen Res. 2014 Apr 15;9(8):837-44. Doi: 10.4103/1673-5374.131600. Apoptosis Is An Obstacle To The Differentiation Of Adipose-Derived Stromal Cells Into Astrocytes.

Chen S, Peng H, Rowat A, Gao F, Zhang Z, Wang P, Zhang W, Wang X, Qu L. Brain Res. 2015 Aug 27;1618:205-11. Doi: 10.1016/J.Brainres.2015.05.027. Epub 2015 May 30. The Effect Of Concentration And Duration Of Normobaric Oxygen In Reducing Caspase-3 ...

Dai C, Li J, Tang S, Li J, Xiao X. Antimicrob Agents Chemother. 2014 Jul;58(7):4075-85. Doi: 10.1128/Aac.00070-14. Epub 2014 May 5. Colistin-Induced Nephrotoxicity In Mice Involves The Mitochondrial, Death Receptor, And Endoplasmic Reticulum Pathw...

Huang C, Lin Y, Su H, Ye D. Neurochem Res. 2015 Jan;40(1):27-35. Doi: 10.1007/S11064-014-1461-5. Epub 2014 Oct 25. Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress And Apoptosis In Pc12 Cell.

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5 Customer Q&As for Anti-Caspase 9/CASP9 Antibody

Question

Do you have any data about PA1595 for use in IF with PFA fixed cells?

Verified customer

Asked: 2020-06-19

Answer

Our lab hasn't performed IF test with PFA fixed cells using the Anti-Caspase 9/CASP9 Antibody (PA1595). Please run a pilot test to see if it would work.

Boster Scientific Support

Answered: 2020-06-22

Question

We have observed staining in human adrenal tissue. Are there any suggestions? Is anti-Caspase 9/CASP9 antibody supposed to stain adrenal tissue positively?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

From literature adrenal tissue does express CASP9. From Uniprot.org, CASP9 is expressed in adrenal tissue, t-cell, stomach cancer, kidney, eye lymph, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP9 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, and Lymph, Pubmed ID: 15489334
Kidney, Pubmed ID: 14702039
Stomach cancer, Pubmed ID: 9890966
T-cell, Pubmed ID: 8900201

Boster Scientific Support

Answered: 2019-09-24

Question

We are currently using anti-Caspase 9/CASP9 antibody PA1595 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2018-12-31

Answer

The anti-Caspase 9/CASP9 antibody (PA1595) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-12-31

Question

We ordered your anti-Caspase 9/CASP9 antibody for IHC on kidney in the past. I am using human, and We are going to use the antibody for WB next. I was wanting to use examining kidney as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-12-29

Answer

I have checked the website and datasheets of our anti-Caspase 9/CASP9 antibody and it seems that PA1595 has been validated on human in both IHC and WB. Thus PA1595 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-12-29

Question

I am interested in using your anti-Caspase 9/CASP9 antibody for activation of caspases through apoptosome-mediated cleavage studies. Has this antibody been tested with western blotting on smmc whole cell lysate? We would like to see some validation images before ordering.

C. Bhatt

Verified customer

Asked: 2014-10-16

Answer

We appreciate your inquiry. This PA1595 anti-Caspase 9/CASP9 antibody is tested on smmc whole cell lysate, cem whole cell lysate, jurkat whole cell lysate, raji whole cell lysate, hela whole cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-10-16

Order DetailsPrice
PA1595

100μg

$370
PA1595-10ug

10μg sample (liquid)

$99
PA1595-Biotin

100 μg Biotin conjugated

$570
PA1595-Cy3

100 μg Cy3 conjugated

$570
PA1595-Dylight488

100 μg Dylight488 conjugated

$570
PA1595-Dylight550

100 μg Dylight550 conjugated

$570
PA1595-Dylight594

100 μg Dylight594 conjugated

$570
PA1595-FITC

100 μg FITC conjugated

$570
PA1595-HRP

100 μg HRP conjugated

$570
PA1595-APC

100 μg APC conjugated

$670
PA1595-PE

100 μg PE conjugated

$670
PA1595-carrier-free

Carrier Free

$370

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