Anti-Caspase-9/CASP9 Antibody Picoband®

Product Name

Anti-Caspase-9/CASP9 Antibody Picoband®

View all CASP9 Antibodies

SKU/Catalog Number

PB9332
PB0285 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-9/CASP9 Antibody Picoband® catalog # PB9332. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-9/CASP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9332)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Caspase-9 recombinant protein (Position: E3-D228). Human Caspase-9 shares 63% amino acid (aa) sequence identity with mouse Caspase-9.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9332 is reactive to CASP9 in Human

Observed Molecular Weight

35 kDa

Calculated molecular weight

46.3 kDa

Background of CASP9

CASP9 is also known as MCH6 or APAF3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9332 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: A549 Whole Cell, SMMC Whole Cell, 293T Whole Cell, JURKAT Whole Cell, RAJI Whole Cell, CEM Whole Cell, HUT Whole Cell

Validation Images & Assay Conditions

See full target information CASP9

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