Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8)

Boster Bio Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8) catalog # M00334-7. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human. Cited in 17 publication(s).

Product Info Summary

SKU: M00334-7
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8)

See all Caspase-3 products

SKU/Catalog Number

M00334-7

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8) catalog # M00334-7. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-7)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

15G8

Isotype

IgG1

Immunogen

E.coli-derived human Caspase-3 recombinant protein (Position: T67-D175). Human Caspase-3 shares 86% and 90% amino acid (aa) sequence identity with mouse and rat Caspase-3, respectively.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00334-7 is reactive to CASP3 in Human

Applications

M00334-7 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For CASP3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P42574

Gene ID

836

Gene Name

CASP3

Full Name

Caspase-3

Weight

31.608kDa

Superfamily

peptidase C14A family

Alternative Names

Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CASP3, check out the CASP3 Infographic

CASP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CASP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00334-7 has been cited in 17 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu DX,Li PP,Guo JP,Li LL,Guo B,Jiao HB,Wu JH,Chen JM.Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy.Oncol Lett.2019 Jan;17(1):323-331.doi:10.3892/ol.2018.9584.Epub 2018 Oct 16.PMID:30655770; PMCID: PMC6313222.
Species: Human
M00334-7 usage in article: APP:WB, SAMPLE: HEPG2 CELL, DILUTION:1:500

Zhang Z,Wang J,Zhu Y,Zhang H,Wang H.Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism.Mol Med Rep.2019 Nov;20(5):4612-4622.doi:10.3892/mmr.2019.10716.Epub 2019 Oct 1.PMID:31702040; PMCID:PMC6797977.
Species: Human,Rat
M00334-7 usage in article: APP:WB, SAMPLE:MITOCHONDRIAL TISSUE AND H9C2 CELL, DILUTION:1:1000

Yu Y,Wang Y,Fei X,Song Z,Xie F,Yang F,Liu X,Xu Z,Wang G.All-Trans Retinoic Acid Prevented Vein Grafts Stenosis by Inhibiting Rb-E2F Mediated Cell Cycle Progression and KLF5-RARα Interaction in Human Vein Smooth Muscle Cells.Cardiovasc Drugs Ther.2020 Oct
Species: Human,New Zealand White Rabbit
M00334-7 usage in article: APP:WB, SAMPLE:HUVMSCS, DILUTION:1:1000

Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
M00334-7 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:1000

Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
M00334-7 usage in article: APP:WB, SAMPLE:A431 CELLS, DILUTION:NA

Maternal separation exacerbates Alzheimer's disease-like behavioral and pathological changes in adult APPswe/PS1dE9 mice

MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/%u03B2-catenin signaling

In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

Enhanced anti-apoptosis and gut epithelium protection function of acidic fibroblast growth factor after cancelling of its mitogenic activity

Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction

An J, Liu T, She R, Wu Q, Tian J, Shi R, Hao W, Ren X, Yang Y, Lu Y, Yang Y, Wu Y. Oncotarget. 2017 Dec 17;9(4):4475-4484. doi: 10.18632/oncotarget.23381. eCollection 2018 Jan 12. Replication of hepatitis E virus in the ovary and promotion of oocy...

Liu Nb, Peng T, Pan C, Yao Yy, Shen B, Leng J. World J Gastroenterol. 2005 Oct 28;11(40):6281-7. Overexpression Of Cyclooxygenase-2 In Human Hepg2, Bel-7402 And Smmc-7721 Hepatoma Cell Lines And Mechanism Of Cyclooxygenase-2 Selective Inhibitor Ce...

Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.

Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L. Neural Regen Res. 2014 Apr 15;9(8):837-44. Doi: 10.4103/1673-5374.131600. Apoptosis Is An Obstacle To The Differentiation Of Adipose-Derived Stromal Cells Into Astrocytes.

Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...

Chu Sh, Ma Yb, Feng Df, Zhang H, Qiu Jh, Zhu Za. Oncol Lett. 2011 Nov;2(6):1139-1142. Epub 2011 Aug 4. Elevated Expression Of Solute Carrier Family 22 Member 18 Increases The Sensitivity Of U251 Glioma Cells To Bcnu.

Tian W, Wei T, Li B, Wang Z, Zhang N, Xie G. Plos One. 2014 May 6;9(5):E96775. Doi: 10.1371/Journal.Pone.0096775. Ecollection 2014. Pathway Of Programmed Cell Death And Oxidative Stress Induced By ??-Hydroxybutyrate In Dairy Cow Abomasum Smooth Mu...

Have you used Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8)

Question

Would M00334-7 anti-Caspase-3 antibody (monoclonal, 15G8) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

As indicated on the product datasheet, M00334-7 anti-Caspase-3 antibody (monoclonal, 15G8) as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-10

Question

I have a question about product M00334-7, anti-Caspase-3 antibody (monoclonal, 15G8). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-25

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00334-7 anti-Caspase-3 antibody (monoclonal, 15G8), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-25

Question

Is this M00334-7 anti-Caspase-3 antibody (monoclonal, 15G8) reactive to the isotypes of CASP3?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

The immunogen of M00334-7 anti-Caspase-3 antibody (monoclonal, 15G8) is E.coli-derived human Caspase-3 recombinant protein (Position: T67-D175). Human Caspase-3 shares 86% and 90% amino acid (aa) sequence identity with mouse and rat Caspase-3, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-28

Question

We bought anti-Caspase-3 antibody (monoclonal, 15G8) for WB on t-cell in a previous project. I am using human, and We intend to use the antibody for IHC-P next. I was wanting to use examining t-cell as well as lymph in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

I have checked the website and datasheets of our anti-Caspase-3 antibody (monoclonal, 15G8) and it seems that M00334-7 has been validated on human in both WB and IHC-P. Thus M00334-7 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-12-12

Question

Is a blocking peptide available for product anti-Caspase-3 antibody (monoclonal, 15G8) (M00334-7)?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

We do provide the blocking peptide for product anti-Caspase-3 antibody (monoclonal, 15G8) (M00334-7). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-08

Question

I see that the anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-08

Question

I have attached the WB image, lot number and protocol we used for lymph using anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-26

Question

Would anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7 work for IHC-P with lymph?

N. Rodriguez

Verified customer

Asked: 2019-05-14

Answer

According to the expression profile of lymph, CASP3 is highly expressed in lymph. So, it is likely that anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7 will work for IHC-P with lymph.

Boster Scientific Support

Answered: 2019-05-14

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7. Let me know if you need anything else.

P. Thomas

Verified customer

Asked: 2019-03-01

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-01

Question

We need to test anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7 on human lymph for research purposes, then I may be interested in using anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-12-03

Answer

The products we sell, including anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-12-03

Question

Is there a BSA free version of anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7 available?

Verified Customer

Verified customer

Asked: 2018-06-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-06-13

Question

I was wanting to use your anti-Caspase-3 antibody (monoclonal, 15G8) for IHC-P for human lymph on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymph identification?

M. Williams

Verified customer

Asked: 2017-06-12

Answer

You can see on the product datasheet, M00334-7 anti-Caspase-3 antibody (monoclonal, 15G8) has been validated for IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-12

Question

We have seen staining in human tongue. Any tips? Is anti-Caspase-3 antibody (monoclonal, 15G8) supposed to stain tongue positively?

C. Johnson

Verified customer

Asked: 2016-06-16

Answer

Based on literature tongue does express CASP3. Based on Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-06-16

Question

We were satisfied with the WB result of your anti-Caspase-3 antibody (monoclonal, 15G8). However we have been able to see positive staining in lymph cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP3 supposed to be expressed?

A. Walker

Verified customer

Asked: 2015-12-09

Answer

From what I have seen in literature, lymph does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2015-12-09

Question

We are currently using anti-Caspase-3 antibody (monoclonal, 15G8) M00334-7 for human tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?

C. Krishna

Verified customer

Asked: 2014-04-01

Answer

The anti-Caspase-3 antibody (monoclonal, 15G8) (M00334-7) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-04-01

$280

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
M00334-7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.