This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
PPT1 antibody (M02690) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Flow Cytometry analysis of THP-1 cells using anti- PPT1 antibody (M02690).
Overlay histogram showing THP-1 cells stained with M02690 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PPT1 Antibody (M02690, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with M02690 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PPT1 Antibody (M02690, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-PPT1 Antibody Picoband® (monoclonal, 10F3)
PPT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IHC, WB |
Tested Applications
PPT1 antibody was positively detected by WB in:
human K562 whole cell, human HepG2 whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PPT1 antibody was positively detected by IHC in:
human intestinal cancer tissue, human lung cancer tissue, human mammary cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PPT1 antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PPT1 Antibody Picoband® (monoclonal, 10F3)
View all PPT1 Antibodies
View all PPT1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
10F3
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PPT1, different from the related mouse and rat sequences by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
34 kDa
Calculated Molecular Weight
34.2 kDa
Background of PPT1
Palmitoyl-protein thioesterase 1 (PPT-1), also known as palmitoyl-protein hydrolase 1, is an enzyme that in humans is encoded by the PPT1 gene. PPT-1 is a member of the palmitoyl protein thioesterase family. The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-PPT1 Antibody Picoband® (monoclonal, 10F3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02690)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
94%
High Homology
Sheep
94%
High Homology
Cow
91%
High Homology
Dog
91%
High Homology
Mouse
88%
Moderate Homology
Rat
88%
Moderate Homology
Zebrafish
65%
Low homology
Drosophila
56%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PPT1 antibody
Immunohistochemistry (IHC) protocol for PPT1 antibody
Flow Cytometry (FC) protocol for PPT1 antibody
Publications
Application:
Species:
High PPT1 expression predicts poor clinical outcome and PPT1 inhibitor DC661 enhances sorafenib sensitivity in hepatocellular carcinoma
Published: 2022-03-11
Journal: Cancer Cell International
Application: WB,IF
Impact Factor: 6.43
Species: Human
PMID: 35277179
Review
Have you used Anti-PPT1 Antibody Picoband® (monoclonal, 10F3)?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-PPT1 Antibody Picoband® (monoclonal, 10F3) performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M02690
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026