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Product Info Summary
| SKU: | A02512-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD100 Antibody
SKU/Catalog Number
A02512-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD100 Antibody catalog # A02512-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD100 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02512-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human SEMA4D. AA range:61-110
Reactive Species
A02512-1 is reactive to SEMA4D in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
96.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02512-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western blot analysis of lysate from MCF7 cells, using SEMA4D Antibody.
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Western Blot analysis of MCF7, L929 cells using CD100 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-CD100 Antibody (A02512-1)
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Customer Reviews
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6 Customer Q&As for Anti-CD100 Antibody
Question
My question regards to test anti-CD100 antibody A02512-1 on mouse c1 segment of cervical spinal cord for research purposes, then I may be interested in using anti-CD100 antibody A02512-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The products we sell, including anti-CD100 antibody A02512-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-27
Question
Is a blocking peptide available for product anti-CD100 antibody (A02512-1)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We do provide the blocking peptide for product anti-CD100 antibody (A02512-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-10
Question
Please see the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-CD100 antibody A02512-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
I was wanting to use your anti-CD100 antibody for WB for mouse c1 segment of cervical spinal cord on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse c1 segment of cervical spinal cord identification?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
You can see on the product datasheet, A02512-1 anti-CD100 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse c1 segment of cervical spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
Is this A02512-1 anti-CD100 antibody reactive to the isotypes of SEMA4D?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
The immunogen of A02512-1 anti-CD100 antibody is Synthesized peptide derived from the N-terminal region of human CD100. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-11
Question
We are currently using anti-CD100 antibody A02512-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
A. Zhang
Verified customer
Asked: 2017-01-18
Answer
The anti-CD100 antibody (A02512-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-18
