Anti-CD11b ITGAM Rabbit Monoclonal Antibody

ITGAM antibody

Boster Bio Anti-CD11b ITGAM Rabbit Monoclonal Antibody catalog # M00144-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00144-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-CD11b ITGAM Rabbit Monoclonal Antibody

View all ITGAM Antibodies

SKU/Catalog Number

M00144-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD11b ITGAM Rabbit Monoclonal Antibody catalog # M00144-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD11b ITGAM Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00144-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GC-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD11b

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00144-1 is reactive to ITGAM in Human, Mouse, Rat

Applications

M00144-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

127.179kDa

Background of ITGAM

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)

Gene Name

ITGAM

Full Name

Integrin alpha-M

Weight

127.179kDa

Superfamily

integrin alpha chain family

Alternative Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC 1; MAC1; MAC-1; Mac1, alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha polypeptide; Mo1, alpha subunit; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6 ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 antigen-like family member B|CR-3 alpha chain|antigen CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage antigen alpha polypeptide|neutrophil adherence receptor alpha-M subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ITGAM, check out the ITGAM Infographic

ITGAM infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00144-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Promoted interaction of C/EBP%u03B1 with demethylated Cxcr3 gene promoter contributes to neuropathic pain in mice

Have you used Anti-CD11b ITGAM Rabbit Monoclonal Antibody?

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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

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Find answers in Q&As, reviews.

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16 Customer Q&As for Anti-CD11b ITGAM Rabbit Monoclonal Antibody

Question

My question regarding product M00144-1, anti-CD11b Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Jackson

Verified customer

Asked: 2020-04-30

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00144-1 anti-CD11b Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-30

Question

My boss were content with the WB result of your anti-CD11b Rabbit Monoclonal antibody. However we have been able to see positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

According to literature, liver does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2020-04-28

Question

I was wanting to use your anti-CD11b Rabbit Monoclonal antibody for IHC for mouse blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse blood identification?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

As indicated on the product datasheet, M00144-1 anti-CD11b Rabbit Monoclonal antibody has been validated for IF, IHC, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-26

Question

Is this M00144-1 anti-CD11b Rabbit Monoclonal antibody reactive to the isotypes of ITGAM?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

The immunogen of M00144-1 anti-CD11b Rabbit Monoclonal antibody is A synthesized peptide derived from human CD11b. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-17

Question

Does anti-CD11b Rabbit Monoclonal antibody M00144-1 work on zebrafish IF with bone marrow cell?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

Our lab technicians have not tested anti-CD11b Rabbit Monoclonal antibody M00144-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CD11b Rabbit Monoclonal antibody M00144-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish bone marrow cell in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CD11b Rabbit Monoclonal antibody M00144-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-04

Question

We are currently using anti-CD11b Rabbit Monoclonal antibody M00144-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

The anti-CD11b Rabbit Monoclonal antibody (M00144-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-23

Question

I see that the anti-CD11b Rabbit Monoclonal antibody M00144-1 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-01

Question

Would M00144-1 anti-CD11b Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-02-21

Answer

It shows on the product datasheet, M00144-1 anti-CD11b Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-02-21

Question

Here is the WB image, lot number and protocol we used for blood using anti-CD11b Rabbit Monoclonal antibody M00144-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-01-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-21

Question

Do you have a BSA free version of anti-CD11b Rabbit Monoclonal antibody M00144-1 available?

W. Zhang

Verified customer

Asked: 2018-10-24

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD11b Rabbit Monoclonal antibody M00144-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-10-24

Question

Will anti-CD11b Rabbit Monoclonal antibody M00144-1 work for IHC with blood?

Verified Customer

Verified customer

Asked: 2017-06-28

Answer

According to the expression profile of blood, ITGAM is highly expressed in blood. So, it is likely that anti-CD11b Rabbit Monoclonal antibody M00144-1 will work for IHC with blood.

Boster Scientific Support

Answered: 2017-06-28

Question

We have tried in the past anti-CD11b Rabbit Monoclonal antibody for IF on blood in a previous project. I am using mouse, and I plan to use the antibody for IHC next. My question regards examining blood as well as bone marrow cell in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

A. Krishna

Verified customer

Asked: 2017-05-03

Answer

I have checked the website and datasheets of our anti-CD11b Rabbit Monoclonal antibody and I see that M00144-1 has been validated on mouse in both IF and IHC. Thus M00144-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-05-03

Question

Is a blocking peptide available for product anti-CD11b Rabbit Monoclonal antibody (M00144-1)?

N. Krishna

Verified customer

Asked: 2015-08-17

Answer

We do provide the blocking peptide for product anti-CD11b Rabbit Monoclonal antibody (M00144-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-08-17

Question

We have seen staining in mouse bone marrow cell. Any tips? Is anti-CD11b Rabbit Monoclonal antibody supposed to stain bone marrow cell positively?

J. Jones

Verified customer

Asked: 2013-11-11

Answer

According to literature bone marrow cell does express ITGAM. According to Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2013-11-11

Question

I would like to test anti-CD11b Rabbit Monoclonal antibody M00144-1 on mouse blood for research purposes, then I may be interested in using anti-CD11b Rabbit Monoclonal antibody M00144-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Krishna

Verified customer

Asked: 2013-06-26

Answer

The products we sell, including anti-CD11b Rabbit Monoclonal antibody M00144-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-06-26

Order DetailsPrice
M00144-1

100μL

$370
M00144-1-30ul

30μL sample

$99
M00144-1-carrier-free

Carrier Free

$370

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M00144-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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