SKU | EK0695 |
---|---|
Size | 96wells/kit, with removable strips. |
Reactivity | Mouse |
Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
Sensitivity | <10pg/ml |
Assay Range | 0.78ng/ml-50ng/ml |
Overview
Product Name | Mouse CD14 PicoKine™ ELISA Kit |
---|---|
SKU/Catalog Number | EK0695 |
Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Size | 96wells/kit, with removable strips. |
Description | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD14. 96wells/kit, with removable strips. |
Cite This Product | Mouse CD14 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0695) |
Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility. |
Sensitivity | <10pg/ml |
Assay Range | 0.78ng/ml-50ng/ml |
Immunogen | Expression system for standard: NSO; Immunogen sequence: A18-P345 |
Reactivity | Mouse |
Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
Antibody Clonalities | Capture Antibody | Detection Antibody: monoclonal antibody from rat|polyclonal antibody from goat |
Assay Details
Kit Components
Catalog number | Description | Quantity |
EK0695-CAP | 96-well plate precoated with anti-Mouse CD14 antibody | 1 |
EK0695-ST | lyophilized recombinant Mouse CD14 standard | 50ng/tube |
EK0695-DA | biotinylated anti-Mouse CD14 antibody | 130ul |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
AR1106-1 | sample diluent buffer | 30ml |
AR1106-2 | antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Mouse CD14 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.069 | 0.135 | 0.180 | 0.274 | 0.475 | 0.858 | 1.283 | 2.118 |
Intra/Inter Assay Precision
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1809 | 4760 | 24340 | 1781 | 4305 | 24426 |
Standard deviation | 103.11 | 261.8 | 1217 | 119.32 | 236.77 | 1392.28 |
CV(%) | 5.7% | 5.5% | 5% | 6.7% | 5.5% | 5.7% |
Reproducibility
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 1809 | 1708 | 1610 | 1761 | 1722 | 73.87 | 4.2% |
Sample 2 | 4760 | 4159 | 4699 | 4655 | 4568 | 239.2 | 5.2% |
Sample 3 | 24340 | 25258 | 25930 | 26104 | 25408 | 692.82 | 2.7% |
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Target Info
Protein Target Info (Source: Uniprot.org)
Uniprot Id | P10810 |
---|---|
Gene Name | CD14 |
Protein Name | Monocyte differentiation antigen CD14 |
Alternative Names | Monocyte differentiation antigen CD14;Myeloid cell-specific leucine-rich glycoprotein;CD14;Cd14; |
Subcellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
Molecular Weight | 39204 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
Protein Function | In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity). . |
---|---|
Research Areas | Cell Type Markers, Hematopoietic Progenitors, Immunology, Innate Immunity, Mesenchymal Stem Cells, Myeloid, Stem Cells, Tlr Signaling *You can search these to find other products in these research areas. |
Background | CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. |
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (EK0695)
Promotion:
Order 4+ of the same ELISA kit and get 20% off| Order 20+ of the same ELISA kit and get 30% off .Troubleshooting
Boster Guarantee

Guaranteed product quality
We promise all of our products perform as described in datasheets.
Publications
Customer Q&As
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
Keywords: component, ingredient
Keywords: expiration, storage, temperature, size