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Product Info Summary
| SKU: | M00812-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB, LMNX |
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Product info
Product Name
Anti-CD163 Mouse Monoclonal Antibody [Clone ID: OTI2B12]
SKU/Catalog Number
M00812-2
Size
100 µl
Description
Boster Bio CD163 mouse monoclonal antibody, clone OTI2B12 (formerly 2B12). Catalog# M00812-2. Tested in IF, IHC, LMNX, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-CD163 Mouse Monoclonal Antibody [Clone ID: OTI2B12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00812-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2B12
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human CD163 (NP_004235) produced in HEK293T cell.
Reactive Species
M00812-2 is reactive to CD163 in Human
Calculated molecular weight
125.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00812-2 is guaranteed for IF, IHC, WB, LMNX Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:200~4000
IHC 1:150
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Western blot analysis of extracts (35ug) from 1 tissue lysate by using anti-CD163 monoclonal antibody (1:250).
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-CD163 mouse monoclonal antibody (M00812-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163.
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CD163 Luminex with 3C1 Capture and 2B12 Detection (M00812-2) Antibodies. Substrate used: full length HEK293 cells expressed recombinant protein of human CD163.
Specific Publications For Anti-CD163 Mouse Monoclonal Antibody [Clone ID: OTI2B12] (M00812-2)
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