Click image to see more details
Product Info Summary
| SKU: | A02164-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-PAFAH/PLA2G7 Antibody Picoband®
SKU/Catalog Number
A02164-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PAFAH/PLA2G7 Antibody Picoband® catalog # A02164-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAFAH/PLA2G7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02164-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PAFAH recombinant protein (Position: A176-N415).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02164-2 is reactive to PLA2G7 in Human
Observed Molecular Weight
50 kDa
Calculated molecular weight
50.1 kDa
Background of PLA2G7
Lipoprotein-associated phospholipase A2 (Lp-PLA2), also known as platelet-activating factor acetylhydrolase (PAF-AH), is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene. Lp-PLA2 is a 45-kDa protein of 441 amino acids. It is one of several PAF acetylhydrolases. In the blood it travels mainly with low-density lipoprotein (LDL). Less than 20% is associated with high-density lipoprotein HDL. It is an enzyme produced by inflammatory cells and hydrolyzes oxidized phospholipids in LDL. Lp-PLA2 is platelet-activating factor (PAF) acetylhydrolase, a secreted enzyme that catalyzes the degradation of PAF to inactive products by hydrolysis of the acetyl group at the sn-2 position, producing the biologically inactive products LYSO-PAF and acetate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02164-2 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: human Caco-2 whole cell, human SW620 whole cell
Validation Images & Assay Conditions
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Western blot analysis of PAFAH using anti-PAFAH antibody (A02164-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysate,
Lane 2: human SW620 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH antigen affinity purified polyclonal antibody (Catalog # A02164-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAFAH at approximately 50KD. The expected band size for PAFAH is at 50KD.
Specific Publications For Anti-PAFAH/PLA2G7 Antibody Picoband® (A02164-2)
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Customer Q&As
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5 Customer Q&As for Anti-PAFAH/PLA2G7 Antibody Picoband®
Question
I see that the anti-PAFAH/PLA2G7 antibody A02164-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-19
Question
Would anti-PAFAH/PLA2G7 antibody A02164-2 work on feline WB with lymphoma?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
Our lab technicians have not validated anti-PAFAH/PLA2G7 antibody A02164-2 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PAFAH/PLA2G7 antibody A02164-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline lymphoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-13
Question
Is a blocking peptide available for product anti-PAFAH/PLA2G7 antibody (A02164-2)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
We do provide the blocking peptide for product anti-PAFAH/PLA2G7 antibody (A02164-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-19
Question
Here is the WB image, lot number and protocol we used for blood using anti-PAFAH/PLA2G7 antibody A02164-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-28
Question
We are currently using anti-PAFAH/PLA2G7 antibody A02164-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
E. Walker
Verified customer
Asked: 2016-02-23
Answer
The anti-PAFAH/PLA2G7 antibody (A02164-2) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-23
