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Product Info Summary
| SKU: | M03149 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD272 (BTLA) Mouse Monoclonal Antibody [Clone ID: OTI2E4]
SKU/Catalog Number
M03149
Size
100 µl
Description
Boster Bio BTLA mouse monoclonal antibody,clone OTI2E4. Catalog# M03149. Tested in FC, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-CD272 (BTLA) Mouse Monoclonal Antibody [Clone ID: OTI2E4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03149)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2E4
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human BTLA (NP_861445) produced in HEK293T cell.
Reactive Species
M03149 is reactive to BTLA in Human
Calculated molecular weight
32.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03149 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:4000
IHC 1:150
FC 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTLA.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Flow cytometric analysis of live Sup-B15 cells
Specific Publications For Anti-CD272 (BTLA) Mouse Monoclonal Antibody [Clone ID: OTI2E4] (M03149)
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