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Product Info Summary
| SKU: | A03257-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CD94 KLRD1 Monoclonal Antibody
SKU/Catalog Number
A03257-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD94 KLRD1 Monoclonal Antibody catalog # A03257-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD94 KLRD1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03257-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1H1
Isotype
IgG
Immunogen
Purified recombinant fragment of human CD94 expressed in E. Coli.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03257-1 is reactive to KLRD1 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
20.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03257-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis using CD94 Monoclonal Antibody against recombinant protein.
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Flow cytometric analysis of RAJI cells using CD94 Monoclonal Antibody (green) and negative control (purple).
Specific Publications For Anti-CD94 KLRD1 Monoclonal Antibody (A03257-1)
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Customer Q&As
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6 Customer Q&As for Anti-CD94 KLRD1 Monoclonal Antibody
Question
We are currently using anti-CD94 Monoclonal antibody A03257-1 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The anti-CD94 Monoclonal antibody (A03257-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CD94 Monoclonal antibody A03257-1. Let me know if you need anything else.
C. Parker
Verified customer
Asked: 2019-11-29
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-29
Question
I was wanting to use your anti-CD94 Monoclonal antibody for Flow Cytometry for human leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
As indicated on the product datasheet, A03257-1 anti-CD94 Monoclonal antibody has been validated for Flow Cytometry, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-19
Question
Please see the WB image, lot number and protocol we used for leukocyte using anti-CD94 Monoclonal antibody A03257-1. Please let me know if you require anything else.
J. Miller
Verified customer
Asked: 2018-11-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-09
Question
Is this A03257-1 anti-CD94 Monoclonal antibody reactive to the isotypes of KLRD1?
N. Kulkarni
Verified customer
Asked: 2018-10-12
Answer
The immunogen of A03257-1 anti-CD94 Monoclonal antibody is Purified recombinant fragment of human CD94 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-12
Question
Would A03257-1 anti-CD94 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Thomas
Verified customer
Asked: 2016-12-15
Answer
You can see on the product datasheet, A03257-1 anti-CD94 Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-12-15
