Anti-CD31 PECAM1 Rabbit Monoclonal Antibody

CD31/PECAM-1 antibody

Boster Bio Anti-CD31 PECAM1 Rabbit Monoclonal Antibody catalog # M01513-2. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: M01513-2
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IHC, WB

Product Name

Anti-CD31 PECAM1 Rabbit Monoclonal Antibody

View all CD31/PECAM-1 Antibodies

SKU/Catalog Number

M01513-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD31 PECAM1 Rabbit Monoclonal Antibody catalog # M01513-2. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD31 PECAM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01513-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DAI-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD31

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01513-2 is reactive to PECAM1 in Human

Applications

M01513-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

82.522kDa

Background of CD31/PECAM-1

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For PECAM1 (Source: Uniprot.org, NCBI)

Gene Name

PECAM1

Full Name

Platelet endothelial cell adhesion molecule

Weight

82.522kDa

Alternative Names

adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule PECAM1 CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM platelet and endothelial cell adhesion molecule 1 platelet endothelial cell adhesion molecule|CD31 antigen|platelet endothelial cell adhesion molecule-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PECAM1, check out the PECAM1 Infographic

PECAM1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PECAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01513-2 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

EPCR promotes MGC803 human gastric cancer cell tumor angiogenesis in vitro through activating ERK1/2 and AKT in a PAR1%u2011dependent manner

The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex

Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling

ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer

Chen L, Chan Th, Yuan Yf, Hu L, Huang J, Ma S, Wang J, Dong Ss, Tang Kh, Xie D, Li Y, Guan Xy. J Clin Invest. 2010 Apr;120(4):1178-91. Doi: 10.1172/Jci40665. Epub 2010 Mar 24. Chd1L Promotes Hepatocellular Carcinoma Progression And Metastasis In M...

Qiu H, Yang B, Pei Zc, Zhang Z, Ding K. J Biol Chem. 2010 Oct 15;285(42):32638-46. Doi: 10.1074/Jbc.M110.105544. Epub 2010 Aug 2. Wss25 Inhibits Growth Of Xenografted Hepatocellular Cancer Cells In Nude Mice By Disrupting Angiogenesis Via Blocking...

Zhou S, Yi T, Liu R, Bian C, Qi X, He X, Wang K, Li J, Zhao X, Huang C, Wei Y. Mol Cell Proteomics. 2012 Jul;11(7):M112.017988. Doi: 10.1074/Mcp.M112.017988. Epub 2012 Apr 9. Proteomics Identification Of Annexin A2 As A Key Mediator In The Metasta...

Xiu J, Fan J, Li J, Cui G, Lei W. Biomed Res Int. 2014;2014:785146. Doi: 10.1155/2014/785146. Epub 2014 Jun 10. Different Angiogenic Abilities Of Self-Setting Calcium Phosphate Cement Scaffolds Consisting Of Different Proportions Of Fibrin Glue.

Li J, Zeng G, Qi Y, Tang X, Zhang J, Wu Z, Liang J, Shi L, Liu H, Zhang P. Plos One. 2015 Apr 7;10(4):E0123264. Doi: 10.1371/Journal.Pone.0123264. Ecollection 2015. Xenotransplantation Of Human Adipose-Derived Stem Cells In Zebrafish Embryos.

Wu Q, Chang Y, Zhang L, Zhang Y, Tian T, Feng G, Zhou S, Zheng Q, Han F, Huang F. J Cancer. 2013 Nov 21;4(9):727-35. Doi: 10.7150/Jca.7576. Ecollection 2013. Srpk1 Dissimilarly Impacts On The Growth, Metastasis, Chemosensitivity And Angiogenesis O...

Have you used Anti-CD31 PECAM1 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-CD31 PECAM1 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-CD31 PECAM1 Rabbit Monoclonal Antibody

Question

Will M01513-2 anti-CD31 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

It shows on the product datasheet, M01513-2 anti-CD31 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-19

Question

I have attached the WB image, lot number and protocol we used for leukemic t-cell using anti-CD31 Rabbit Monoclonal antibody M01513-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-26

Question

I am looking for to test anti-CD31 Rabbit Monoclonal antibody M01513-2 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD31 Rabbit Monoclonal antibody M01513-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

The products we sell, including anti-CD31 Rabbit Monoclonal antibody M01513-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-18

Question

My team were well pleased with the WB result of your anti-CD31 Rabbit Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is PECAM1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

From what I have seen in literature, leukemic t-cell does express PECAM1. Generally PECAM1 expresses in cell membrane. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2019-08-15

Question

Does anti-CD31 Rabbit Monoclonal antibody M01513-2 work for WB with leukemic t-cell?

R. Walker

Verified customer

Asked: 2019-05-31

Answer

According to the expression profile of leukemic t-cell, PECAM1 is highly expressed in leukemic t-cell. So, it is likely that anti-CD31 Rabbit Monoclonal antibody M01513-2 will work for WB with leukemic t-cell.

Boster Scientific Support

Answered: 2019-05-31

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD31 Rabbit Monoclonal antibody M01513-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-01

Question

Is a blocking peptide available for product anti-CD31 Rabbit Monoclonal antibody (M01513-2)?

Verified Customer

Verified customer

Asked: 2019-01-30

Answer

We do provide the blocking peptide for product anti-CD31 Rabbit Monoclonal antibody (M01513-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-30

Question

We are currently using anti-CD31 Rabbit Monoclonal antibody M01513-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-21

Answer

The anti-CD31 Rabbit Monoclonal antibody (M01513-2) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-21

Question

Do you have a BSA free version of anti-CD31 Rabbit Monoclonal antibody M01513-2 available?

Verified Customer

Verified customer

Asked: 2017-09-05

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD31 Rabbit Monoclonal antibody M01513-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-09-05

Question

My question regarding product M01513-2, anti-CD31 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-08-28

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01513-2 anti-CD31 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-08-28

Question

We have observed staining in human tendon of biceps brachii. What should we do? Is anti-CD31 Rabbit Monoclonal antibody supposed to stain tendon of biceps brachii positively?

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

From literature tendon of biceps brachii does express PECAM1. From Uniprot.org, PECAM1 is expressed in tendon of biceps brachii, lung, brain, platelet, liver, leukemic t-cell, among other tissues. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2017-07-10

Question

I was wanting to use your anti-CD31 Rabbit Monoclonal antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

As indicated on the product datasheet, M01513-2 anti-CD31 Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-15

Question

Would anti-CD31 Rabbit Monoclonal antibody M01513-2 work on zebrafish WB with lung?

J. Krishna

Verified customer

Asked: 2016-12-21

Answer

Our lab technicians have not validated anti-CD31 Rabbit Monoclonal antibody M01513-2 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CD31 Rabbit Monoclonal antibody M01513-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish lung in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-12-21

Question

Is this M01513-2 anti-CD31 Rabbit Monoclonal antibody reactive to the isotypes of PECAM1?

E. Moore

Verified customer

Asked: 2014-11-26

Answer

The immunogen of M01513-2 anti-CD31 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD31. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-11-26

Question

I see that the anti-CD31 Rabbit Monoclonal antibody M01513-2 works with WB, what is the protocol used to produce the result images on the product page?

A. Collins

Verified customer

Asked: 2013-12-09

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-12-09

Order DetailsPrice
M01513-2

100μL

$370
M01513-2-30ul

30μL sample

$99
M01513-2-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M01513-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.