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Product Info Summary
| SKU: | M01189-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CD36 Mouse Monoclonal Antibody [Clone ID: OTI3F4]
SKU/Catalog Number
M01189-1
Size
100 µl
Description
Boster Bio Anti-CD36 mouse monoclonal antibody, clone OTI3F4 (formerly 3F4). Catalog# M01189-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD36 Mouse Monoclonal Antibody [Clone ID: OTI3F4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01189-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3F4
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CD36 (NP_000063) produced in HEK293T cell.
Reactive Species
M01189-1 is reactive to CD36 in Human, Rat
Calculated molecular weight
53.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01189-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:50
IF: 1:100
Flow Cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells transfected with either CD36 (Myc-DDK-tagged) overexpress plasmid (Red)
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Anti-CD36 mouse monoclonal antibody (M01189-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD36.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CD36mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CD36mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD36 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36 (1:500).
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Western blot analysis of extracts (35ug) from human placenta tissue by using anti-CD36 monoclonal antibody (1:500).
Specific Publications For Anti-CD36 Mouse Monoclonal Antibody [Clone ID: OTI3F4] (M01189-1)
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