Anti-CD40 Antibody Picoband™

Boster Bio Anti-CD40 Antibody Picoband™ catalog # PB10051. Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB10051
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-CD40 Antibody Picoband™

See all CD40/TNFRSF5 products

SKU/Catalog Number

PB10051

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD40 Antibody Picoband™ catalog # PB10051. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD40 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10051)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human CD40/TNFRSF5 recombinant protein (Position: E21-R193). Human CD40/TNFRSF5 shares 60.2% amino acid (aa) sequence identity with mouse CD40/TNFRSF5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB10051 is reactive to CD40 in Human

Applications

PB10051 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD40 (Source: Uniprot.Org, NCBI)

Uniprot ID

P25942

Gene ID

958

Gene Name

CD40

Full Name

Tumor necrosis factor receptor superfamily member 5

Weight

30.619kDa

Alternative Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface antigen CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD40, check out the CD40 Infographic

CD40 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD40: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PB10051

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15 Customer Q&As for Anti-CD40 Antibody Picoband™

Question

I was wanting to use to test anti-CD40 antibody PB10051 on human kidney for research purposes, then I may be interested in using anti-CD40 antibody PB10051 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

The products we sell, including anti-CD40 antibody PB10051, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-05-06

Question

Would anti-CD40 antibody PB10051 work for WB with kidney?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

According to the expression profile of kidney, CD40 is highly expressed in kidney. So, it is likely that anti-CD40 antibody PB10051 will work for WB with kidney.

Boster Scientific Support

Answered: 2020-02-28

Question

We are currently using anti-CD40 antibody PB10051 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

The anti-CD40 antibody (PB10051) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-02

Question

See below the WB image, lot number and protocol we used for kidney using anti-CD40 antibody PB10051. Please let me know if you require anything else.

G. Huang

Verified customer

Asked: 2019-12-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-18

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-CD40 antibody PB10051. Let me know if you need anything else.

K. Walker

Verified customer

Asked: 2019-06-26

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-26

Question

My question regarding product PB10051, anti-CD40 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-12-19

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10051 anti-CD40 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-19

Question

My team were well pleased with the WB result of your anti-CD40 antibody. However we have seen positive staining in kidney isoform i: cell membrane using this antibody. Is that expected? Could you tell me where is CD40 supposed to be expressed?

J. Anderson

Verified customer

Asked: 2018-07-24

Answer

From literature, kidney does express CD40. Generally CD40 expresses in isoform i: cell membrane. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161

Boster Scientific Support

Answered: 2018-07-24

Question

I was wanting to use your anti-CD40 antibody for WB for human kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney identification?

Verified Customer

Verified customer

Asked: 2018-01-19

Answer

You can see on the product datasheet, PB10051 anti-CD40 antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-19

Question

Our lab used your anti-CD40 antibody for WB on left coronary artery in a previous project. I am using human, and We are going to use the antibody for ELISA next. We are interested in examining left coronary artery as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

D. Li

Verified customer

Asked: 2017-11-29

Answer

I looked at the website and datasheets of our anti-CD40 antibody and it seems that PB10051 has been validated on human in both WB and ELISA. Thus PB10051 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-11-29

Question

Is a blocking peptide available for product anti-CD40 antibody (PB10051)?

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

We do provide the blocking peptide for product anti-CD40 antibody (PB10051). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-06-15

Question

Will PB10051 anti-CD40 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-05-29

Answer

It shows on the product datasheet, PB10051 anti-CD40 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-05-29

Question

Is this PB10051 anti-CD40 antibody reactive to the isotypes of CD40?

M. Evans

Verified customer

Asked: 2017-02-08

Answer

The immunogen of PB10051 anti-CD40 antibody is E. coli-derived human CD40/TNFRSF5 recombinant protein (Position: E21-R193). Human CD40/TNFRSF5 shares 60.2% amino acid (aa) sequence identity with mouse CD40/TNFRSF5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-02-08

Question

We have been able to see staining in human ovary. Do you have any suggestions? Is anti-CD40 antibody supposed to stain ovary positively?

D. Jones

Verified customer

Asked: 2017-02-01

Answer

From literature ovary does express CD40. From Uniprot.org, CD40 is expressed in left coronary artery, kidney, ovary, leukocyte, lymphoma urinary bladder carcinoma, among other tissues. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161

Boster Scientific Support

Answered: 2017-02-01

Question

Do you have a BSA free version of anti-CD40 antibody PB10051 available?

M. Walker

Verified customer

Asked: 2016-03-15

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD40 antibody PB10051 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-03-15

Question

I see that the anti-CD40 antibody PB10051 works with WB, what is the protocol used to produce the result images on the product page?

E. Williams

Verified customer

Asked: 2014-06-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-06-05

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PB10051
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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