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Product Info Summary
| SKU: | PB9298 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NR5A2/LRH1 Antibody Picoband®
SKU/Catalog Number
PB9298
PB0326 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NR5A2/LRH1 Antibody Picoband® catalog # PB9298. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NR5A2/LRH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9298)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NR5A2 recombinant protein (Position: K44-R237). Human NR5A2 shares 95% amino acid (aa) sequence identity with both mouse and rat NR5A2.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9298 is reactive to NR5A2 in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
61.3 kDa
Background of NR5A2
NR5A2 (nuclear receptor subfamily 5, group A, member 2) also known as liver receptor homolog-1 (LRH-1) is a protein that in humans is encoded by the NR5A2 gene. LRH-1 is a member of the nuclear receptor family of intracellulartranscription factors. LRH-1 plays a critical role in the regulation of development,cholesterol transport, bile acid homeostasis andsteroidogenesis. LRH-1 is important for maintaining pluripotence of stem cells during embryonic development. Liver receptor homolog-1 has been shown to interact with the small heterodimer partner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9298 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human A549 whole cell, human CACO-2 whole cell, human MCF-7 whole cell, human HepG2 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Western blot analysis of NR5A2 using anti-NR5A2 antibody (PB9298).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR5A2 antigen affinity purified polyclonal antibody (PB9298) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NR5A2 at approximately 70 kDa. The expected band size for NR5A2 is at 61 kDa.
Specific Publications For Anti-NR5A2/LRH1 Antibody Picoband® (PB9298)
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1 Customer Q&As for Anti-NR5A2/LRH1 Antibody Picoband®
Question
We are currently using anti-NR5A2/LRH1 antibody PB9298 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
D. Li
Verified customer
Asked: 2013-10-30
Answer
The anti-NR5A2/LRH1 antibody (PB9298) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-30
