Skip to the beginning of the images gallery

CD44 antibody (M00052-3) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 17 Publications

Datasheet MSDS Request COA

m00052 3 cd44 primary antibodies fcm testing 3

View All Images (8)

Flow Cytometry analysis of U87 cells using anti-CD44 antibody (M00052-3).
Overlay histogram showing U87 cells stained with M00052-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- CD44 Antibody (M00052-3, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Designing a Western blot for CD44? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the CD44 Western Blot Guide

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-CD44 Picoband® Antibody (monoclonal, 7H7)

CD44 antibody

17 Publications

Reactive Species	Host	Application
Human, Rat	Mouse	Flow Cytometry, IF, ICC, WB

Tested Applications

CD44 antibody was positively detected by WB in:

human Hela whole cell, human A549 whole cell, rat C6 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CD44 antibody was positively detected by ICC/IF in:

A431 cell

CD44 antibody was positively detected by FC in:

U87 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

Human

Tested

Rat

Tested

Monkey

Predicted

Rabbit

Predicted

Dog

Predicted

Mouse

Predicted

Bovine

Predicted

Sheep

Predicted

Product Information

Product Name

Anti-CD44 Picoband® Antibody (monoclonal, 7H7)
View all CD44 Antibodies

Form

Lyophilized

Uniprot

P16070

Gene ID

960

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Monoclonal

Clone Number

7H7

Isotype

Mouse IgG2b

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CD44, different from the related mouse and rat sequences by two amino acids.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

82 kDa

Calculated Molecular Weight

81.5 kDa

Background of CD44

CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Cite This Product

Anti-CD44 Picoband® Antibody (monoclonal, 7H7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00052-3)

Protocols

Western Blot protocol for CD44 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CD44 antibody

Review Protocol

Flow Cytometry (FC) protocol for CD44 antibody

Review Protocol

Publications

Hoxc10‐mediated ‘positional memory’ regulates cartilage formation subsequent to femoral heterotopic grafting

Published: 2024-10-21

Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

Application: IF

Impact Factor: 4.30

Species: Rat

PMID: 39434203

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-CD44 Picoband® Antibody (monoclonal, 7H7)

CD44 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Hoxc10‐mediated ‘positional memory’ regulates cartilage formation subsequent to femoral heterotopic grafting

Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes

Overexpression of microRNA-205-5p exerts suppressive effects on stem cell drug resistance in gallbladder cancer by down-regulating PRKCE

Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery

Antioxidant and Cytoprotective Effects of the Di-O-Caffeoylquinic Acid Family: The Mechanism, Structure–Activity Relationship, and Conformational Effect

Protective Mechanism of the Antioxidant Baicalein toward Hydroxyl Radical-Treated Bone Marrow-Derived Mesenchymal Stem Cells

Two phenolic antioxidants in Suoyang enhance viability of •OH-damaged mesenchymal stem cells: comparison and mechanistic chemistry

Effect and mechanism of wedelolactone as antioxidant-coumestan on OH-treated mesenchymal stem cells

Differentiation of Mesenchymal Stem Cells into Neural Stem Cells Using Cerebrospinal Fluid

Colorectal Cancer Progression Is Associated with Accumulation of Th17 Lymphocytes in Tumor Tissues and Increased Serum Levels of Interleukin-6

Genistein Promotion of Osteogenic Differentiation through BMP2/SMAD5/RUNX2 Signaling

HER2 Interacts With CD44 to Up-regulate CXCR4 via Epigenetic Silencing of microRNA-139 in Gastric Cancer Cells

Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation

Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells

Prognostic significance of Survivin and CD44v6 in laryngeal cancer surgical margins

Articular cartilage metabolism in patients with Kashin–Beck Disease: an endemic osteoarthropathy in China

Effects of body-resistance strengthening and tumor-suppressing granules on immune adhesion function of red blood cells and expression of metastasis protein CD44 in tumor cells of patients with esophageal carcinoma

Review

Have you used Anti-CD44 Picoband® Antibody (monoclonal, 7H7)?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality