Anti-Vinculin/VCL Antibody Picoband™

Boster Bio Anti-Vinculin/VCL Antibody Picoband™ catalog # A01207. Tested in WB applications. This antibody reacts with Human. Cited in 6 publication(s).

Product Info Summary

SKU: A01207
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Vinculin/VCL Antibody Picoband™

See all Vinculin products

SKU/Catalog Number

A01207

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Vinculin/VCL Antibody Picoband™ catalog # A01207. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Vinculin/VCL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01207)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Vinculin recombinant protein (Position: E930-Q1134). Human Vinculin shares 87.7% amino acid (aa) sequence identity with both mouse and rat Vinculin.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01207 is reactive to VCL in Human

Applications

A01207 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For VCL (Source: Uniprot.Org, NCBI)

Uniprot ID

P18206

Gene ID

7414

Gene Name

VCL

Full Name

Vinculin

Weight

123.799kDa

Superfamily

vinculin/alpha-catenin family

Alternative Names

CMD1W; CMH15; metavinculin; MVCL; VCL; Vinculin VCL CMD1W, CMH15, HEL114, MV, MVCL vinculin vinculin|epididymis luminal protein 114|epididymis secretory sperm binding protein|meta-vinculin|metavinculin|vinculin isoform meta-VCL

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VCL, check out the VCL Infographic

VCL infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VCL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01207 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang K,Xing ZH,Jiang QW,Yang Y,Huang JR,Yuan ML,Wei MN,Li Y,Wang ST,Liu K,Shi Z.Targeting uPAR by CRISPR/Cas9 System Attenuates Cancer Malignancy and Multidrug Resistance.Front Oncol.2019 Feb 27;9:80.doi:10.3389/fonc.2019.00080.PMID:30873379;PMCID:PMC6400983.
Species: Human
A01207 usage in article: APP:WB, SAMPLE:HCT8/T CELL AND KBV200 CELL, DILUTION:NA

Dong Y,Chen Y,Zhu D,Shi K,Ma C,Zhang W,Rocchi P,Jiang L,Liu X.Self-assembly of amphiphilic phospholipid peptide dendrimer-based nanovectors for effective delivery of siRNA therapeutics in prostate cancer therapy.J Control Release.2020 Jun 10;322:416-425.d
Species: Human,Mouse
A01207 usage in article: APP:WB, SAMPLE:PC-3 CELL, DILUTION:NA

Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells

Yao Q, An Y, Hou W, Cao YN, Yao MF, Ma NN, Hou L, Zhang H, Liu HJ, Zhang B. Oncotarget. 2017 Nov 30;8(65):109632-109645. doi: 10.18632/oncotarget.22759. eCollection 2017 Dec 12. LRP6 promotes invasion and metastasis of colorectal cancer through cy...

Shen Z, Lu D, Li Q, Zhang Z, Zhu Y. Biomed Res Int. 2015;2015:871202. Doi: 10.1155/2015/871202. Epub 2015 Mar 8. Synthesis And Characterization Of Biodegradable Polyurethane For Hypopharyngeal Tissue Engineering.

Su F,, Liu Ss,, Ma Jl, Wang Ds, E Ll, Liu Hc. Stem Cell Res Ther. 2015 Mar 12;6:22. Doi: 10.1186/S13287-015-0023-3. Enhancement Of Periodontal Tissue Regeneration By Transplantation Of Osteoprotegerin-Engineered Periodontal Ligament Stem Cells.

Have you used Anti-Vinculin/VCL Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Vinculin/VCL Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-Vinculin/VCL Antibody Picoband™

Question

We need to test anti-Vinculin/VCL antibody A01207 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Vinculin/VCL antibody A01207 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

L. Patel

Verified customer

Asked: 2020-04-29

Answer

The products we sell, including anti-Vinculin/VCL antibody A01207, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-29

Question

We are currently using anti-Vinculin/VCL antibody A01207 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

The anti-Vinculin/VCL antibody (A01207) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-13

Question

Will anti-Vinculin/VCL antibody A01207 work for WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

According to the expression profile of cervix carcinoma erythroleukemia, VCL is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Vinculin/VCL antibody A01207 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2020-03-18

Question

Is a blocking peptide available for product anti-Vinculin/VCL antibody (A01207)?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

We do provide the blocking peptide for product anti-Vinculin/VCL antibody (A01207). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-27

Question

Can you help my question with product A01207, anti-Vinculin/VCL antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01207 anti-Vinculin/VCL antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-14

Question

Is there a BSA free version of anti-Vinculin/VCL antibody A01207 available?

Verified Customer

Verified customer

Asked: 2019-04-25

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Vinculin/VCL antibody A01207 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-25

Question

Is this A01207 anti-Vinculin/VCL antibody reactive to the isotypes of VCL?

Verified Customer

Verified customer

Asked: 2018-12-28

Answer

The immunogen of A01207 anti-Vinculin/VCL antibody is E.coli-derived human Vinculin recombinant protein (Position: E930-Q1134). Human Vinculin shares 87.7% amino acid (aa) sequence identity with both mouse and rat Vinculin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-12-28

Question

Would A01207 anti-Vinculin/VCL antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-04-25

Answer

It shows on the product datasheet, A01207 anti-Vinculin/VCL antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-04-25

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Vinculin/VCL antibody A01207. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-01-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-10

Question

I was wanting to use your anti-Vinculin/VCL antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2017-11-30

Answer

You can see on the product datasheet, A01207 anti-Vinculin/VCL antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-30

Question

I see that the anti-Vinculin/VCL antibody A01207 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-11-30

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-11-30

Question

We are interested in using your anti-Vinculin/VCL antibody for cell adhesion studies. Has this antibody been tested with western blotting on a549 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-08-01

Answer

We appreciate your inquiry. This A01207 anti-Vinculin/VCL antibody is tested on a549 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-08-01

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Vinculin/VCL antibody A01207. Let me know if you need anything else.

G. Miller

Verified customer

Asked: 2017-01-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-01-16

Question

My boss were content with the WB result of your anti-Vinculin/VCL antibody. However we have seen positive staining in prostate cell membrane using this antibody. Is that expected? Could you tell me where is VCL supposed to be expressed?

S. Johnson

Verified customer

Asked: 2016-08-26

Answer

Based on literature, prostate does express VCL. Generally VCL expresses in cell membrane. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348

Boster Scientific Support

Answered: 2016-08-26

Question

We have seen staining in human prostate. Do you have any suggestions? Is anti-Vinculin/VCL antibody supposed to stain prostate positively?

P. Carter

Verified customer

Asked: 2014-01-02

Answer

From literature prostate does express VCL. From Uniprot.org, VCL is expressed in myometrium, endothelial cell, retina, prostate, fetal brain cortex, uterus, platelet, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348

Boster Scientific Support

Answered: 2014-01-02

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
A01207
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.