This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
VCL antibody (A01207-1) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
Filter:
Flow Cytometry analysis of Hela cells using anti-VCL antibody (A01207-1).
Overlay histogram showing Hela cells stained with A01207-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCL Antibody (A01207-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Hela cells stained with A01207-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCL Antibody (A01207-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-Vinculin/VCL Antibody Picoband®
VCL antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
VCL antibody was positively detected by WB in:
human Hela whole cell, human U-87MG whole cell, human HT1080 whole cell, human HEK293 whole cell, human SK-OV-3 whole cell, monkey COS-7 whole cell, human K562 whole cell, human SGC-7901 whole cell, rat heart tissue, mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
VCL antibody was positively detected by IHC in:
human lung cancer tissue, human lung cancer tissue, human mammary cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
VCL antibody was positively detected by IF in:
human mammary cancer tissue
VCL antibody was positively detected by FC in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Monkey
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Vinculin/VCL Antibody Picoband®
View all VCL Antibodies
View all VCL Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Vinculin/VCL recombinant protein (Position: M1-E149).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
124 kDa
Calculated Molecular Weight
123.8 kDa
Background of VCL
In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. It is mapped to 10q22.2. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Cite This Product
Anti-Vinculin/VCL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01207-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Dog
96%
High Homology
Zebrafish
94%
High Homology
Drosophila
64%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for VCL antibody
Immunohistochemistry (IHC) protocol for VCL antibody
Immunofluorescence (IF) protocol for VCL antibody
Flow Cytometry (FC) protocol for VCL antibody
Publications
Application:
Species:
Exosome Nanotherapy Rescues Male Fertility by Delivering Integrin β1 to Repair Environmental Stress-Damaged Testes
Published: 2026-06-08
Journal: MOLECULAR PHARMACEUTICS
Application: IHC,IF,WB
Impact Factor: 4.90
Species: Mouse
PMID: 42258346
A chitosan-based thermosensitive hydrogel incorporating propranolol nanoparticles to promote early osseointegration and peri-implant soft-tissue healing
Published: 2026-03-06
Journal: RSC Advances
Application: IF
Impact Factor: 4.60
Species: Human
PMID: 41799915
RSAD2: A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus
Published: 2025-02-20
Journal: Cell Reports Medicine
Application: WB
Impact Factor: 10.60
Species: Mouse,Human
PMID: 39983716
Biodegradable Magnesium alloy Janus membrane with surface-selective osteoinduction and soft tissue healing properties in guided bone regeneration
Published: 2025-02-09
Journal: Acta Biomaterialia
Application: IF
Impact Factor: 9.60
Species: Mouse,Human
PMID: 39933642
Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR−/− Mice
Published: 2023-12-20
Journal: ENDOCRINOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38123514
Integrating network pharmacology and bioinformatics to explore and experimentally verify the regulatory effect of Buyang Huanwu decoction on glycolysis and angiogenesis after cerebral infarction
Published: 2023-10-06
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 37806535
Inhibiting the Activity of ABCG2 by KU55933 in Colorectal Cancer
Published: 2022-11-01
Journal: Recent Patents on Anti-Cancer Drug Discovery
Application: WB
Impact Factor: 3.04
Species: Human
PMID: 35023460
Sustained delivery efficiency of curcumin through ZSM-5 nanozeolites/electrospun nanofibers for counteracting senescence of human adipose-derived stem cells
Published: 2021-10-21
Journal: JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
Application: WB
Impact Factor: 3.98
Species: Human
Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo
Published: 2018-04-25
Journal: Acta Biomaterialia
Application: IF
Impact Factor: 6.38
Species: Rat,Human
PMID: 29702291
Toxicity analysis of various Pluronic F-68-coated carbon nanotubes on mesenchymal stem cells
Published: 2016-03-10
Journal: CHEMICO-BIOLOGICAL INTERACTIONS
Impact Factor: 2.62
Species: Rat
PMID: 26970603
Monitoring of TGF-β 1-Induced Human Lung Adenocarcinoma A549 Cells Epithelial-Mesenchymal Transformation Process by Measuring Cell Adhesion Force with a Microfluidic Device
Published: 2015-09-22
Journal: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Application: IF
Impact Factor: 1.74
Species: Human
PMID: 26394790
Review
Have you used Anti-Vinculin/VCL Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Vinculin/VCL Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01207-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026