Anti-CD44 Rabbit Monoclonal Antibody

CD44 antibody

Boster Bio Anti-CD44 Rabbit Monoclonal Antibody catalog # M00052-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse. Cited in 8 publication(s).

Product Info Summary

SKU: M00052-1
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-CD44 Rabbit Monoclonal Antibody

View all CD44 Antibodies

SKU/Catalog Number

M00052-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD44 Rabbit Monoclonal Antibody catalog # M00052-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD44 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00052-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AGI-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD44

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00052-1 is reactive to CD44 in Human, Mouse

Applications

M00052-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

81.538kDa

Background of CD44

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CD44 (Source: Uniprot.org, NCBI)

Gene Name

CD44

Full Name

CD44 antigen

Weight

81.538kDa

Alternative Names

CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1 CD44 CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1 CD44 molecule (Indian blood group) CD44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|Indian blood group antigen|cell surface glycoprotein CD44|chondroitin sulfate proteoglycan 8|epican|extracellular matrix receptor III|hematopoietic cell E- and L-selectin ligand|heparan sulfate proteoglycan|homing function and Indian blood group system|hyaluronate receptor|phagocytic glycoprotein 1|soluble CD44

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD44, check out the CD44 Infographic

CD44 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD44: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00052-1 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M00052-1 usage in article: APP:WB, SAMPLE:MSC, DILUTION:NA

Zhang GF,Wu JC,Wang HY,Jiang WD,Qiu L. Overexpression of microRNA-205-5p exerts suppressive effects on stem cell drug resistance in gallbladder cancer by down-regulating PRKCE. Biosci Rep.2020 Sep 30;40(9): BSR20194509.doi:10.1042/BSR20194509.PMID: 328698
Species: Human,Mouse
M00052-1 usage in article: APP:FLOW CYTOMETRY, SAMPLE:GBC STEM CELLS, DILUTION:NA

Kubatka P,Kello M,Kajo K,Samec M,Liskova A,Jasek K,Koklesova L,Kuruc T,Adamkov M,Smejkal K,Svajdlenka E,Solar P,Pec M,Büsselberg D,Sadlonova V,Mojzis J.Rhus coriaria L.(Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Bre
Species: Human,Mouse,Rat
M00052-1 usage in article: APP:IHC, SAMPLE:MAMMARY TISSUE, DILUTION:NA

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Rat nasal respiratory mucosa-derived ectomesenchymal stem cells differentiate into Schwann-like cells promoting the differentiation of PC12 cells and %u2026

Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells

Lentivirus-mediated PLC?1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma

Dai J, Li Y, Zhou H, Chen J, Chen M, Xiao Z. Int J Biol Sci. 2013 Nov 21;9(10):1089-98. Doi: 10.7150/Ijbs.7367. Ecollection 2013. Genistein Promotion Of Osteogenic Differentiation Through Bmp2/Smad5/Runx2 Signaling.

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16 Customer Q&As for Anti-CD44 Rabbit Monoclonal Antibody

Question

I was wanting to use your anti-CD44 Rabbit Monoclonal antibody for IHC for human peripheral blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human peripheral blood identification?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

As indicated on the product datasheet, M00052-1 anti-CD44 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human peripheral blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-27

Question

Is this M00052-1 anti-CD44 Rabbit Monoclonal antibody reactive to the isotypes of CD44?

N. Brown

Verified customer

Asked: 2020-04-15

Answer

The immunogen of M00052-1 anti-CD44 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD44. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-15

Question

We are currently using anti-CD44 Rabbit Monoclonal antibody M00052-1 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

The anti-CD44 Rabbit Monoclonal antibody (M00052-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-12

Question

My question regarding product M00052-1, anti-CD44 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00052-1 anti-CD44 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-05

Question

My question regards to test anti-CD44 Rabbit Monoclonal antibody M00052-1 on human peripheral blood for research purposes, then I may be interested in using anti-CD44 Rabbit Monoclonal antibody M00052-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

The products we sell, including anti-CD44 Rabbit Monoclonal antibody M00052-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-03

Question

Does M00052-1 anti-CD44 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

You can see on the product datasheet, M00052-1 anti-CD44 Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-09

Question

Does anti-CD44 Rabbit Monoclonal antibody M00052-1 work on horse IF with myeloid leukemia cell?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

Our lab technicians have not validated anti-CD44 Rabbit Monoclonal antibody M00052-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CD44 Rabbit Monoclonal antibody M00052-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse myeloid leukemia cell in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-21

Question

We have observed staining in mouse lung. What should we do? Is anti-CD44 Rabbit Monoclonal antibody supposed to stain lung positively?

S. Kulkarni

Verified customer

Asked: 2018-10-23

Answer

From what I have seen in literature lung does express CD44. From what I have seen in Uniprot.org, CD44 is expressed in parotid gland, reticulocyte, myeloid leukemia cell, keratinocyte, lymphoblast, mammary carcinoma, articular cartilage, colon adenocarcinoma retinal pigment epithelium, spinal cord, pancreas retinal pigment epithelium, glial tumor, peripheral blood, foreskin, lung, plasma, cervix carcinoma, t-cell, liver, leukemic t-cell, among other tissues. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2018-10-23

Question

Is a blocking peptide available for product anti-CD44 Rabbit Monoclonal antibody (M00052-1)?

Verified Customer

Verified customer

Asked: 2018-07-09

Answer

We do provide the blocking peptide for product anti-CD44 Rabbit Monoclonal antibody (M00052-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-07-09

Question

I have attached the WB image, lot number and protocol we used for peripheral blood using anti-CD44 Rabbit Monoclonal antibody M00052-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-02-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-02-23

Question

Our team were well pleased with the WB result of your anti-CD44 Rabbit Monoclonal antibody. However we have seen positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is CD44 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-01-24

Answer

Based on literature, liver does express CD44. Generally CD44 expresses in cell membrane. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2018-01-24

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-CD44 Rabbit Monoclonal antibody M00052-1. Let me know if you need anything else.

A. Zhang

Verified customer

Asked: 2017-06-29

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-29

Question

Will anti-CD44 Rabbit Monoclonal antibody M00052-1 work for IHC with peripheral blood?

D. Miller

Verified customer

Asked: 2016-01-07

Answer

According to the expression profile of peripheral blood, CD44 is highly expressed in peripheral blood. So, it is likely that anti-CD44 Rabbit Monoclonal antibody M00052-1 will work for IHC with peripheral blood.

Boster Scientific Support

Answered: 2016-01-07

Question

Do you have a BSA free version of anti-CD44 Rabbit Monoclonal antibody M00052-1 available?

D. Gonzalez

Verified customer

Asked: 2015-05-28

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD44 Rabbit Monoclonal antibody M00052-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-05-28

Question

We ordered your anti-CD44 Rabbit Monoclonal antibody for IF on spinal cord last year. I am using human, and We are going to use the antibody for IHC next. I was wanting to use examining spinal cord as well as parotid gland in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

W. Li

Verified customer

Asked: 2014-12-10

Answer

I have checked the website and datasheets of our anti-CD44 Rabbit Monoclonal antibody and it appears that M00052-1 has been validated on human in both IF and IHC. Thus M00052-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-12-10

Question

I see that the anti-CD44 Rabbit Monoclonal antibody M00052-1 works with IHC, what is the protocol used to produce the result images on the product page?

J. Jha

Verified customer

Asked: 2013-02-12

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-02-12

Order DetailsPrice
M00052-1

100μL

$370
M00052-1-30ul

30μL sample

$99
M00052-1-carrier-free

Carrier Free

$370

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M00052-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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