P-selectin Antibodies

P-selectin (SELP)

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes.

The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the first steps in inflammation through interaction with SELPLG.

Gene Information

Human
Gene Name:	SELP
Uniprot:	P16109
Entrez:	6403

Family

Belongs to:
selectin/LECAM family

Alternative Names

CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

Molecular Weight

Mass (kDA):
90.834 kDA

Genomic Context

Human
Location:	1q24.2
Sequence:	1; NC_000001.11 (169588849..169630125, complement)

Tissue Specificity

Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein.

Diseases

• Tissue Adhesions
• Inflammation
• Malignant Neoplasms
• Atherosclerosis
• Cardiovascular Diseases
• Neoplasms
• Heart Diseases
• Inflammatory Response
• Malignant Head And Neck Neoplasm
• Von Willebrand Disease
• Infarction
• Myocardial Infarction
• Head And Neck Neoplasms

• Malignant Neck Neoplasm
• Coronary Artery Disease
• Thrombosis
• Dysequilibrium Syndrome
• Coronary Heart Disease
• Diabetes Mellitus
• Neoplasm Metastasis

Interacting Proteins

• SELPLG

Pathways

• Cell Adhesion
• Platelet Activation
• Pathogenesis
• Inflammatory Response
• Platelet Aggregation
• Aging
• Cell Activation
• Immune Response
• Endothelial Cell Activation
• Angiogenesis
• Transport
• Hypersensitivity
• Virulence

• Spermatogenesis
• Fermentation
• Translation
• Cell Cycle
• Fibrinolysis
• Innate Immune Response
• Cognition

PTMs

• Phosphorylation
• Nitration

• Cleavage
• Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SELP

Best Uses Of The SELP Marker

This article will provide details about monoclonal antibodies that are anti-PECAM-1 (Endothelial Cell Marker), and anti-CD34. It also provides optimization tips and troubleshooting tips to help you get the best results from your experiments. Although there are many things that could cause problems with your experiments and experiments, proper controls can aid in eliminating them.

Boster Bio's anti-CD34 monoclonal antibody

Boster Bio's monclonal anti-CD34 antibody utilizes the SELP marker to identify human hematopoietic stem cells. It has been extensively tested on various platforms, with positive samples as well as unpublished ones. It is highly specific and has a high affinity, making it a great instrument for the isolation and purification human HSCs. The monoclonal antibody also recognizes various cell types, which are essential for HSC differentiation.

Anti-CD31/PECAM-1 (Endothelial Cell Marker) monoclonal antibody

Anti-CD31/PECAM-1, which is a highly specific anti-endothelial antibody, is available. This glycoprotein is a transmembrane that is expressed on a diverse variety of cells, including endothelial cells. This anti-endothelial cell antibody is particularly useful in identifying stem cells and the concentration of them for bone transplantation. It is highly specific for vascular endothelial cell. Moreover, it reacts with benign, normal and malignant endothelial cells. Therefore, the role of CD31 in tumor angiogenesis is crucial because high levels of CD31 expression on endothelial cells indicates the rapid growth of tumors.

The polyclonal antibody anti-CD31/PECAM-1 from Boster Bio recognizes the protein in vitro. It is a binder to CD31 intracellular immunoreceptor that carries out its adhesive properties and is associated with an endothelial marker. The polyclonal antibody is validated in clinical trials as a reliable tool to monitor the activity of endothelial cells in patients.

CD31 is an inhibitory receptor that regulates the activity of T and B cells. It is abundantly expressed on platelets and endothelial cells. It has multiple functions in the vascular biology and function of platelets. It is also vital in the regulation and migration of leukocytes along the venular walls.

The Anti-CD31/PECAM-1 monoclonal antibody from Boster Bio has been validated by IHC-P and IF. It reacts with Human, Monkey, and Rabbit tissues. It is a useful tool for animal, veterinary, and human research. It is highly sensitive and enables rapid detection of the protein targeted.

PECAM can be found in platelets, monocytes and neutrophils, as well as in a few T-cell varieties. It regulates the function of RhoA. It is therefore a good anti-apoptotic antibody. It is a very specific antibody, which is vital for cancer research.

Tips for optimization

Boster Bio optimization tips are among the most effective ways to increase the performance and efficiency of your experiments. These tips and guides will answer a variety of questions regarding your experiments, such as:

Troubleshooting guides

You need to pinpoint what is the root of the issue to solve it. A new component or peripheral can be the cause of the issue, as well as an error code. If the error is random, you must look into the settings on your computer to determine its cause. Try to reproduce the issue, if possible. The issue may be present at any time, or intermittently throughout the day or week.