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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Mouse B7-1/CD80 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse B7-1/CD80 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cd80 in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse B7-1/CD80 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0708)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: D37-K245
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0708 is reactive to Cd80 in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
EK0708 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0708-CAP||Anti-Mouse Cd80 Pre-coated 96-well strip microplate||1|
|EK0708-ST||Mouse Cd80 Standard||2 vials, 10 ng/tube|
|EK0708-DA||Mouse Cd80 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse B7-1/CD80 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse B7-1/CD80 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of B7-1/CD80 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Cd80 (Source: Uniprot.Org, NCBI)
T-lymphocyte activation antigen CD80
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80 Cd80|B71, Cd28, Cd28l, Ly-5, Ly-53, Ly5, Ly53, MIC17, TSA1|CD80 antigen|T-lymphocyte activation antigen CD80|B7 protein|activation B7-1 antigen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cd80, check out the Cd80 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd80: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0708 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Oncolytic measles virus encoding interleukin-12 mediates potent antitumor effects through T cell activation
Du Hy, Dong Lh, Zhao Bj, Fu J, Wang Qq, Chen F, Ou L, Li N, Sun X, Tang Zm, Song Hf. Acta Pharmacol Sin. 2012 Aug;33(8):1047-54. Doi: 10.1038/Aps.2012.54. Epub 2012 Jun 25. Immunostimulatory And Anti-Neoplasm Effects Of A Novel Palindrome Cpg Olig...
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