Anti-cdc25C (Ab-216) Antibody

CDC25C antibody

Boster Bio Anti-cdc25C (Ab-216) Antibody (Catalog # A01343-1). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A01343-1
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-cdc25C (Ab-216) Antibody

View all CDC25C Antibodies

SKU/Catalog Number

A01343-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-cdc25C (Ab-216) Antibody (Catalog # A01343-1). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-cdc25C (Ab-216) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01343-1)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa.214~218 (S-P-S-M-P) derived from Human cdc25C.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01343-1 is reactive to CDC25C in Human

Applications

A01343-1 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

53.365kDa

Background of CDC25C

cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3(4): 341-348.
Ferguson AM. et al. (2005) Mol Cell Biol. 25(7): 2853-2860.
Donzelli M. et al. (2003) EMBO Rep. 4(7): 671-677.
Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99(4): 1990-1995.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

Gene/Protein Information For CDC25C (Source: Uniprot.org, NCBI)

Gene Name

CDC25C

Full Name

M-phase inducer phosphatase 3

Weight

53.365kDa

Superfamily

MPI phosphatase family

Alternative Names

CDC 25; CDC25; CDC25C; cell division cycle 25 homolog C (S. cerevisiae); cell division cycle 25 homolog C (S. pombe); cell division cycle 25C; Dual specificity phosphatase Cdc25C; EC 3.1.3.48; mitosis inducer CDC25; M-phase inducer phosphatase 3; phosphotyrosine phosphatase CDC25C CDC25, PPP1R60 cell division cycle 25C M-phase inducer phosphatase 3|CDC25 homolog C|dual specificity phosphatase CDC25C|mitosis inducer CDC25|phosphotyrosine phosphatase|protein phosphatase 1, regulatory subunit 60

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDC25C, check out the CDC25C Infographic

CDC25C infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDC25C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A01343-1

100ul

$360

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