Anti-CDC27 phospho T244 Antibody

Product Name

Anti-CDC27 phospho T244 Antibody

View all CDC27, D0S1430E Antibodies

SKU/Catalog Number

P03905

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-CDC27 phospho T244 Antibody (Catalog # P03905). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-CDC27 phospho T244 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03905)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion surrounding T244 of Human CDC27.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P03905 is reactive to CDC27, D0S1430E in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

91.9 kDa

Background of CDC27, D0S1430E

Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P03905 is guaranteed for ELISA, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:8,000 - 1:30,000
IP: 1:100
WB: 1:300 - 1:2,000
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 92 kDa in size corresponding to CDC27 by western blotting in the appropriate cell lysate or extract.  Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pT244 of CDC27.

Validation Images & Assay Conditions

See full target information CDC27, D0S1430E

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