Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine™

Mouse E-Cadherin 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cdh1 in cell culture supernatants, serum and plasma (heparin). . Sensitivity: 15pg/ml.

Product Info Summary

SKU: EK0562
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine™

See all Cdh1 products

SKU/Catalog Number

EK0562

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse E-Cadherin 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cdh1 in cell culture supernatants, serum and plasma (heparin). . Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0562)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D157-V709

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0562 is reactive to Cdh1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0562 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0562-CAP Anti-Mouse Cdh1 Pre-coated 96-well strip microplate 1
EK0562-ST Mouse Cdh1 Standard 2 vials, 10 ng/tube
EK0562-DA Mouse Cdh1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0500.1320.2040.3740.6451.1181.8652.342

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cdh1, Dilution ratio of 1:200, concentration in serum is 262-339ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2421644563121717715410
Standard deviation14.52118.36360.3814.53146.99394.93
CV (%)6%7.2%6.4%6.7%8.3%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cdh1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12422472462422442.270.9%
Sample 216441884178816011729113.046.5%
Sample 356315520514549735317268.195%
*number of samples for each test n=16.

Gene/Protein Information For Cdh1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P09803

Gene ID

12550

Gene Name

Cdh1

Full Name

Cadherin-1

Weight

98.256kDa

Alternative Names

Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin Cdh1|AA960649, ARC-1, E-ca, E-cad, E-cadh, Eca, Ecad, L-C, L-CAM, UVO, Um|cadherin 1|cadherin-1|E-cadherin|epithelial cadherin|uvomorulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cdh1, check out the Cdh1 Infographic

Cdh1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cdh1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated CDH1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CDH1 antigen?

Verified Customer

Verified customer

Asked: 2019-12-08

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-08

Question

Q: What is the optimal O.D. value for CDH1 ELISA kit? I performed your CDH1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CDH1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CDH1 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of CDH1 that can be declared/interpreted as positive by the above standard is 15pg/ml.

Boster Scientific Support

Answered: 2019-12-02

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

H. Jha

Verified customer

Asked: 2019-06-07

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-06-07

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. White

Verified customer

Asked: 2018-12-14

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-12-14

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

P. Wang

Verified customer

Asked: 2018-10-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-10-28

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CDH1 in Mouse plasma ? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-10-27

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CDH1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-10-27

Question

Q: how to analyze ELISA data? I have obtained CDH1 level in plasma .

C. Turner

Verified customer

Asked: 2018-03-06

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-03-06

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-06-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-06-25

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