Anti-E Cadherin 1/CDH1 Antibody Picoband®

Western blot analysis of CDH1 using anti-CDH1 antibody (PB9561).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human T47D whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH1 antigen affinity purified polyclonal antibody (PB9561) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDH1 at approximately 130 kDa. The expected band size for CDH1 is at 98 kDa.

Western blot analysis of CDH1 using anti-CDH1 antibody (PB9561).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human cervical cancer tissue lysates,
Lane 2: human cervical cancer adjacent tissue lysates,
Lane 3: human cervical cancer tissue lysates,
Lane 4: human cervical cancer adjacent tissue lysates.
Lane 5: human cervical cancer tissue lysates,
Lane 6: human cervical cancer adjacent tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH1 antigen affinity purified polyclonal antibody (PB9561) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for CDH1 at approximately 130 kDa. The expected band size for CDH1 is at 98 kDa.

IHC analysis of CDH1 using anti-CDH1 antibody (PB9561).
CDH1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CDH1 using anti-CDH1 antibody (PB9561).
CDH1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CDH1 using anti-CDH1 antibody (PB9561).
CDH1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CDH1 using anti-CDH1 antibody (PB9561).
CDH1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CDH1 using anti-CDH1 antibody (PB9561).
CDH1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IF analysis of CDH1 using anti-CDH1 antibody (PB9561).
CDH1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Fluoro488488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Immunohistochemical analysis of dorsolateral prostate E-cadherin, Vimtein, ERα and AR expression in aged rats. The expression of vimentin, ERα and AR increased, and the expression of E-cadherin decreased in BPA-treated groups. ( a – p ) Representative sections of comparable regions are shown for vehicle control rats ( a , e , i , m ), and animals exposed to BPA (10 μg/kg/day) ( b , f , j , n ), BPA (30 μg /kg/day) ( c , g , k , o ), and BPA (90 μg/kg/day) ( d , h , l , p ) (scale bar: 50 μm, ×400). BPA: bisphenol A; AR: androgen receptor; ERα:estrogen receptor-α.
Index in PubMed under a CC BY license. PMID: 29323181

LPC inhibits pulmonary metastasis of CT26 colon cancer. a Schematic view of the experimental procedures of CT26 pulmonary metastatic mouse model. b , c Image and corresponding H&E staining of lung tissue. d Percentage change of body weight. e – g Lung weight, the number of lung tumor nodule, and metastasis rate ( n = 6 mice). h Protein expression of PCNA, vimentin and E-cadherin in lungs ( n = 3 mice). Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01.
Index in PubMed under a CC BY license. PMID: 36774343

Interfering with DLGAP4 inhibits the PPARβ/δ signalling pathway and the expression of proliferation- and metastasis-related proteins. Western blotting was performed to measure the protein expression of DLGAP4, PPARβ/δ, CyclinD1, E-cadherin and N-cadherin in shNC or shDLGAP4 HepG2 and HCCLM3 cells. The data were obtained from the average of three independent experiments. *P < 0.05.
Index in PubMed under a CC BY license. PMID: 36396671

PPI inhibits the metastasis of cervical cancer cells in vivo. ( A , B ) Bioluminescence imaging of various organs exhibiting metastatic or disseminated cancer cells ( A ) and a quantitative analysis of total bioluminescence in each group of organs ( B ). ( C ) H&E staining of various organs in each group. The scale is 100 μm. ( D , E ) The immunofluorescence staining of E-cadherin, VE-cadherin, and Vimentin was performed, followed by data quantification analysis, comparing the control group with the treated group. Six animal samples were used per group ( n = 6), and the results are presented as mean ± S.D. **: p < 0.01; ***: p < 0.001 vs. control. The scale is 100 μm.
Index in PubMed under a CC BY license. PMID: 40429774

PPI inhibits the growth, migration, and invasion of HO-8910PM cells by reversing the EMT progress. ( A ) Cell proliferation was evaluated at 48 h using the MTT assay, and the IC 50 values were determined from dose–response curves generated with GraphPad Prism 8. ( B ) The wound-healing assay was conducted in HO-8910PM cells to evaluate cell migration. Images were captured at 0 and 24 h. The scale is 500 μm. ( C ) The data quantification results of ( B ). ( D ) A transwell migration assay was performed to monitor the rate of cellular migration. The scale is 500 μm. ( E ) The quantification results of ( D ). ( F ) The protein expression levels of E-cadherin, VE-cadherin, and Vimentin in HO-8910PM cells. ( G ) The quantification results of ( F ). ( H ) An immunofluorescence assay was performed to detect E-cadherin, VE-cadherin, and Vimentin expression in HO-8910PM cells treated with PPI. The scale is 100 μm. ( I ) The quantification results of ( H ). Data from six independent experiments are presented as mean ± S.D. *: p < 0.05; **: p < 0.01; ***: p < 0.001 vs. control.
Index in PubMed under a CC BY license. PMID: 40429774