Anti-E Cadherin 1/CDH1 Antibody Picoband™

Boster Bio Anti-E Cadherin 1/CDH1 Antibody Picoband™ catalog # PB9561. Tested in ELISA, IF, IHC-P, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 45 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: PB9561
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, IHC-F, WB

Product Name

Anti-E Cadherin 1/CDH1 Antibody Picoband™

See all CDH1 products

SKU/Catalog Number

PB9561

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-E Cadherin 1/CDH1 Antibody Picoband™ catalog # PB9561. Tested in ELISA, IF, IHC-P, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-E Cadherin 1/CDH1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9561)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9561 is reactive to CDH1 in Human, Mouse, Rat

Applications

PB9561 is guaranteed for ELISA, IF, IHC-P, IHC-F, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat -
Immunofluorescence, 5μg/ml, Human, Mouse
ELISA , 0.1-0.5μg/ml, Human, -

Validation Images & Assay Conditions

Gene/Protein Information For CDH1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P12830

Gene ID

999

Gene Name

CDH1

Full Name

Cadherin-1

Weight

97.456kDa

Alternative Names

Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin CDH1 Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO cadherin 1 cadherin-1|CAM 120/80|E-cadherin 1|cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1, E-cadherin (epithelial)|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|epididymis secretory sperm binding protein|epithelial cadherin|uvomorulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDH1, check out the CDH1 Infographic

CDH1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDH1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9561 has been cited in 45 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Novel HDAC5-interacting motifs of Tbx3 are essential for the suppression of E-cadherin expression and for the promotion of metastasis in hepatocellular carcinoma
Species: Human

Wei X,Bao Y,Zhan X,Zhang L,Hao G,Zhou J,Chen Q.MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis.Int Urol Nephrol.2019 May;51(5):889-896.doi:10.1007/s11255-019-02122-4.Epub 2019 Mar 19.PMID:30888602;PMCID:PMC6499761.
Species: Rat
PB9561 usage in article: APP:IHC, SAMPLE:PERITONEAL TISSUE, DILUTION:NA

Li F,Zhang C,Fu L.PRR14 overexpression promotes cell growth, epithelial to mesenchymal transition and metastasis of colon cancer via the AKT pathway.PLoS One.2019 Oct 9;14(10):e0218839.doi:10.1371/journal.pone.0218839.PMID:31596887;PMCID:PMC6785111.
Species: Human,Mouse
PB9561 usage in article: APP:WB, SAMPLE:HCT116 CELL AND RKO CELL, DILUTION:1:200

Zhou J,Li R,Liu Q,Zhang J,Huang H,Huang C,Zhang G,Zhao Y,Wu T,Tang Q,Huang Y,Zhang Z,Li Y,He J. Blocking 5-LO pathway alleviates renal fibrosis by inhibiting the epithelial-mesenchymal transition. Biomed Pharmacother. 2021 Mar 12;138:111470.doi:10.1016/j.biopha.2021.111470.Epub ahead of print.PMID:33721755.
Species: Human,Mouse
PB9561 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE AND TCMK-1 CELL, DILUTION: NA

Xu,R.,Zhou,X., Li,Y.,Ren,L.,& He,X. (2021).MicroRNA‑181a promotes epithelial‑mesenchymal transition in esophageal squamous cell carcinoma via the TGF‑β/Smad pathway.Molecular Medicine Reports,23,316. https://doi.org/10.3892/mmr.2021.11955
Species: Human
PB9561 usage in article: APP:WB, SAMPLE:TE-1 CELL, DILUTION:1:500-1:2000

Xian-Song Wang,Li Xie,Kaiyu Zheng et al.Human Peripheral Blood-derived Mast Cells Contribute to Epithelial to Mesenchymal Transition in Bronchial Epithelial Cells in the Presence of IL-1β,29 June 2020,PREPRINT (Version 1) available at Research Square [htt
Species: Human
PB9561 usage in article: APP:WB, SAMPLE:16-HBE CELL, DILUTION:1:100

Xiaozhu Tian,Rong Li,Yiming Jiang,Fei Zhao,Zhengsheng Yu,Yiqing Wang,Zixing Dong,Pu Liu,Xiangkai Li,Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake,J
Species: Mouse
PB9561 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND ILEUM, DILUTION:NA

Piao HY,Guo S,Wang Y,Zhang J.Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.Clin Transl Oncol.2020 Jun 9.doi:10.1007/s12094-020-02412-9.Epub ahead of print.PMID:32519176.
Species: Human
PB9561 usage in article: APP:WB, SAMPLE:AGS CELL AND MKN45 CELL, DILUTION:1:200

Xu H,Yu B,Shen W,Jin C,Wang L,Xi Y.Over-expression of long non-coding RNA ZEB2-AS1 may predict poor prognosis and promote the migration, invasion, and epithelial-mesenchymal transition of tumor cells in non-small cell lung cancer.Int J Biol Markers.2020 S
Species: Human
PB9561 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:1:1000

Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
PB9561 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA

Detection of epithelial to mesenchymal transition in airways of a bleomycin induced pulmonary fibrosis model derived from an %u03B1-smooth muscle actin-Cre %u2026

HIF%u20101%u03B1 binding to AEG%u20101 promoter induced upregulated AEG%u20101 expression associated with metastasis in ovarian cancer

Frizzled2 mediates the migration and invasion of human oral squamous cell carcinoma cells through the regulation of the signal transducer and activator of %u2026

Overexpression of SDF-1 activates the NF-%u03BAB pathway to induce epithelial to mesenchymal transition and cancer stem cell-like phenotypes of breast cancer cells

Placental growth factor triggers epithelial%u2010to%u2010mesenchymal transition%u2010like changes in rat type ii alveolar epithelial cells: activation of nuclear factor %u03BAB signalling %u2026

RhoC is essential in TGF-?1 induced epithelial-mesenchymal transition in cervical cancer cells.

MiR-29b/TET1/ZEB2 signaling axis regulates metastatic properties and epithelial-mesenchymal transition in breast cancer cells

Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation

Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells

RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells

Expression of RKIP, E-cadherin and NF-kB p65 in esophageal squamous cell carcinoma and their correlations

Prolonged overexpression of Wnt10b induces epidermal keratinocyte transformation through activating EGF pathway

Connective tissue growth factor is overexpressed in human hepatocellular carcinoma and promotes cell invasion and growth

APC/CCDC20 and APC/C play pivotal roles in the process of embryonic development in Artemia sinica

TGF-?1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21

Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-?B activation

Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition

Regulator of cullins-1 expression knockdown suppresses the malignant progression of muscle-invasive transitional cell carcinoma by regulating mTOR/DEPTOR pathway

Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-?B and TGF-?1/Smad-2/-3 pathways

Isolation and characteristics of CD133?/A2B5+ and CD133?/A2B5? cells from the SHG139s cell line

Li Q, Zhao H, Xia S, Wei H, Chen F, Jin P. Oncotarget. 2017 Dec 21;9(2):2646-2659. doi: 10.18632/oncotarget.23522. eCollection 2018 Jan 5. RUNX2 promotes epithelial differentiation of ADSCs and burn wound healing via targeting E-cadherin

Zhang Q, Bu S, Sun J, Xu M, Yao X, He K, Lai D. Stem Cell Res Ther. 2017 Nov 28;8(1):270. doi: 10.1186/s13287-017-0721-0. Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage

Huang DY, Zheng CC, Pan Q, Wu SS, Su X, Li L, Wu JH, Sun ZY. Sci Rep. 2018 Jan 11;8(1):490. doi: 10.1038/s41598-017-18869-8. Oral exposure of low-dose bisphenol A promotes proliferation of dorsolateral prostate and induces epithelial

Wang J, Zhao H, Tang D, Wu J, Yao G, Zhang Q. Cancer Biother Radiopharm. 2013 May;28(4):283-8. Doi: 10.1089/Cbr.2012.1293. Epub 2013 Apr 25. Overexpressions Of Microrna-9 And Microrna-200C In Human Breast Cancers Are Associated With Lymph Node Met...

Shen R, Xie F, Shen H, Liu Q, Zheng T, Kou X, Wang D, Yang J. Plos One. 2013;8(2):E56542. Doi: 10.1371/Journal.Pone.0056542. Epub 2013 Feb 20. Negative Correlation Of Liv-1 And E-Cadherin Expression In Hepatocellular Carcinoma Cells.

Shen H, Qin H, Guo J. Mol Biol Rep. 2009 Apr;36(4):653-9. Doi: 10.1007/S11033-008-9225-4. Epub 2008 Mar 11. Concordant Correlation Of Liv-1 And E-Cadherin Expression In Human Breast Cancer Cell Mcf-7.

Jin B, Guo B, Che G, Sun Y, Liu Y, Zhang X. Microsc Res Tech. 2012 Aug;75(8):1150-3. Doi: 10.1002/Jemt.22080. Epub 2012 Jun 18. Germline Stem Cell Lineage Tracing By Free-Floating Immunofluorescent Assay Of Mouse Seminiferous Tubule.

Wang M, Zhang J, Song X, Liu W, Zhang L, Wang X, Lv C. Food Chem Toxicol. 2013 Jun;56:450-8. Doi: 10.1016/J.Fct.2013.03.004. Epub 2013 Mar 14. Astaxanthin Ameliorates Lung Fibrosis In Vivo And In Vitro By Preventing Transdifferentiation, Inhibitin...

Deng B, Zhang Y, Zhang S, Wen F, Miao Y, Guo K. Tumour Biol. 2015 Sep;36(10):8065-73. Doi: 10.1007/S13277-015-3483-2. Epub 2015 May 15. Microrna-142-3P Inhibits Cell Proliferation And Invasion Of Cervical Cancer Cells By Targeting Fzd7.

Li Q, Lv Ll, Wu M, Zhang Xl, Liu H, Liu Bc. J Cell Biochem. 2013 Mar;114(3):632-8. Doi: 10.1002/Jcb.24405. Dexamethasone Prevents Monocyte-Induced Tubular Epithelial-Mesenchymal Transition In Hk-2 Cells.

Tang R, Yang C, Tao Jl, You Yk, An N, Li Sm, Wu Hl, Liu Hf. Cell Biol Int. 2011 Sep;35(9):953-9. Doi: 10.1042/Cbi20100668. Epithelial-Mesenchymal Transdifferentiation Of Renal Tubular Epithelial Cells Induced By Urinary Proteins Requires The Activ...

Han P, Fu Y, Luo M, He J, Liu J, Liao J, Tian D, Yan W. Dig Dis Sci. 2014 May;59(5):992-1000. Doi: 10.1007/S10620-013-2992-3. Epub 2014 Jan 18. Bves Inhibition Triggers Epithelial-Mesenchymal Transition In Human Hepatocellular Carcinoma.

Duan F, Lin M, Li C, Ding X, Qian G, Zhang H, Ge S, Fan X, Li J. Cancer Biol Ther. 2014 May;15(5):544-59. Doi: 10.4161/Cbt.28157. Epub 2014 Mar 11. Effects Of Inhibition Of Hedgehog Signaling On Cell Growth And Migration Of Uveal Melanoma Cells.

Xia H, Cheung Wk, Sze J, Lu G, Jiang S, Yao H, Bian Xw, Poon Ws, Kung Hf, Lin Mc. J Biol Chem. 2010 Nov 19;285(47):36995-7004. Doi: 10.1074/Jbc.M110.133744. Epub 2010 Sep 7. Mir-200A Regulates Epithelial-Mesenchymal To Stem-Like Transition Via Zeb...

Li H, Chen L, Zeng S, Li X, Zhang X, Lin C, Zhang M, Xie S, He Y, Shu S, Yang L, Tang S, Fu X. Exp Cell Res. 2015 Mar 1;332(1):67-77. Doi: 10.1016/J.Yexcr.2015.01.014. Epub 2015 Jan 30. Matrigel Basement Membrane Matrix Induces Eccrine Sweat Gland...

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1 Reviews For Anti-E Cadherin 1/CDH1 Antibody Picoband™

0

Immunocytochemistry for Anti-E Cadherin 1/CDH1 Antibody

Excellent

The antibody worked perfectly, Great sensitivity

Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
SampleMouse Liver
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Verified customer

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6 Customer Q&As for Anti-E Cadherin 1/CDH1 Antibody Picoband™

Question

Are there any optimizations that you suggest or Antigen retrieval for PB9561?

Verified customer

Asked: 2021-01-27

Answer

For the Anti-E Cadherin 1/CDH1 Antibody Picoband™ (PB9561), AR0022 is recommended to be used for Antigen retrieval. https://www.bosterbio.com/antigen-retrieval-reagent-ar0022-boster.html

Boster Scientific Support

Answered: 2021-01-28

Question

Our lab want to know about using your anti-E Cadherin/CDH1 antibody for regulation of gene expression studies. Has this antibody been tested with western blotting on placenta tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

We appreciate your inquiry. This PB9561 anti-E Cadherin/CDH1 antibody is tested on human ileum tissue, placenta tissue, tissue lysate, hela whole cell lysate, mouse ileum tissue, ileum organoid tissue, colon organoid tissue. It is guaranteed to work for ELISA, IF, IHC-P, IHC-F, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-20

Question

Our lab used your anti-E Cadherin/CDH1 antibody for IHC-F on hippocampus tongue in a previous experiment. I am using mouse, and I plan to use the antibody for IHC-P next. We want examining hippocampus tongue as well as pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

I viewed the website and datasheets of our anti-E Cadherin/CDH1 antibody and it appears that PB9561 has been tested on mouse in both IHC-F and IHC-P. Thus PB9561 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-12

Question

We are currently using anti-E Cadherin/CDH1 antibody PB9561 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

The anti-E Cadherin/CDH1 antibody (PB9561) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-05

Question

We have seen staining in rat liver. Do you have any suggestions? Is anti-E Cadherin/CDH1 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

Based on literature liver does express CDH1. Based on Uniprot.org, CDH1 is expressed in jejunal mucosa, pancreas, liver, epidermal carcinoma, hippocampus tongue, placenta, brain, among other tissues. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454

Boster Scientific Support

Answered: 2019-06-17

Question

Our team were content with the WB result of your anti-E Cadherin/CDH1 antibody. However we have observed positive staining in epidermal carcinoma adherens junction using this antibody. Is that expected? Could you tell me where is CDH1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-12-17

Answer

From literature, epidermal carcinoma does express CDH1. Generally CDH1 expresses in cell junction, adherens junction. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454

Boster Scientific Support

Answered: 2018-12-17

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