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CDH1 antibody (PB9561) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 73 Publications

pb9561 cdh1 primary antibodies ihc testing 1

IHC analysis of CDH1 using anti-CDH1 antibody (PB9561).
CDH1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-E Cadherin 1/CDH1 Antibody Picoband®

Name: Anti-E Cadherin 1/CDH1 Antibody Picoband®
Brand: Boster Bio
SKU: PB9561
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

CDH1 antibody

73 Publications|

2 Reviews

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, IF, IHC, ICC, WB

Tested Applications

CDH1 antibody was positively detected by WB in:

human MCF-7 whole cell, human A431 whole cell, human HepG2 whole cell, human T47D whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CDH1 antibody was positively detected by IHC in:

human placenta tissue, human hepatocellular carcinoma tissue, mouse colon tissue, rat colon tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CDH1 antibody was positively detected by ICC/IF in:

MCF-7 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Mouse

Tested

Recommend

Rat

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-E Cadherin 1/CDH1 Antibody Picoband®
View all CDH1 Antibodies

Form

Lyophilized

Uniprot

P12830

Gene ID

999

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

130 kDa

Calculated Molecular Weight

97.5 kDa

Background of CDH1

CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Cite This Product

Anti-E Cadherin 1/CDH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9561)

Protocols

Western Blot protocol for CDH1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CDH1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CDH1 antibody

Review Protocol

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

Published: 2026-08-26

Journal: CLINICAL AND EXPERIMENTAL MEDICINE

Application: WB

Impact Factor: 4.50

Species: Human,Mouse

SKU	PB9561
Application	Western Blot
Sample	human cervical cancer and adjacent tissues
Sample Processing Description	Three samples each of human cervical cancer and adjacent tissues were collected, and total protein was extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	E Cadherin 1/CDH1 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary	Based on this study, the expression of E-cadherin in human cervical cancer and adjacent tissues was compared, and results from three cases showed that E-cadherin levels were significantly higher in adjacent tissues than in cancer tissues.

SKU	PB9561
Application	Immunohistochemistry (paraffin-embedded)
Blocking step	5% BSA as a blocking agent for 30 min at 37°C
Sample	Mouse Liver
Fixative	Fixed with 4% paraformaldehyde
Primary Ab Incubation	4°C overnight
Primary Ab Incubation diluent	5% BSA in TBS
Primary Ab Concentration	2ug/ml
Secondary Antibody	SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution	The kit was ready to use, no dilution needed
Secondary Ab Incubation	4°C overnight

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Anti-E Cadherin 1/CDH1 Antibody Picoband®

CDH1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

Betulinic Acid Enema Alleviates Colitis by Targeting Vitamin D Receptor to Protect the Intestinal Mucosal Barrier and Modulate Mucosa-Associated Microbiota

STEAP1 Suppresses Oral Squamous Cell Carcinoma by Targeting Wnt/β‐Catenin Signalling and EMT

YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR–HIF1α–LHDA Axis

Role of Ca2+‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/EDNRB

Macrophage exosomal ADAM10 mediates alveolar epithelial apoptosis induced by wood smoke PM2.5

Geniposide enhances cisplatin sensitivity in non-small cell lung cancer through PUMA-mediated apoptotic pathway

Mechanisms of IL-17A Neutralisation in Alleviating Renal Fibrosis and Inflammation in Spontaneously Hypertensive Rats

GABPB1 activates transcription of ITPR3 and EMT in OSCC progression

Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens

Downregulation of the biological macromolecule Spondin-2 disrupts maternal-fetal immune tolerance and trophoblast invasion, driving unexplained recurrent pregnancy loss

Apoptosis-promoting autophagy mediates anti-migratory effects of unsymmetrical bisacridines in pancreatic cancer cells

Development of an Effective Drug Carrier for Targeted Therapy in Lung Adenocarcinoma Using ROS-responsive Micelles

4EBP1 phosphorylation mediates TGF-β1–induced hepatic fibrosis and upregulates HIF-1α expression

Injectable hydrogel for controlled anlotinib delivery and enhanced anti-tumor therapy in non-small cell lung cancer

Polyphyllin I Inhibits the Metastasis of Cervical Cancer Through the Regulation of the β-Catenin Signaling Pathway

TDH-11 inhibits the proliferation and colonization of colorectal cancer by reducing the activity of HDAC

Impact of SLC16A8 on tumor microenvironment and angiogenesis in colorectal cancer: New therapeutic target insights

Synergistic effects of bloom helicase (BLM) inhibitor AO/854 with cisplatin in prostate cancer

Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma

Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer

Neuropeptide substance P attenuates colitis by suppressing inflammation and ferroptosis via the cGAS-STING signaling pathway

Fabrication of sac-like hydrogel membranes for replicating curved tissue barriers on chips

Consistent analysis of differentially expressed genes across 7 cell types in papillary thyroid carcinoma

SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion

A microfluidic lung-on-a-chip based on biomimetic hydrogel membrane

CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling

Litchi procyanidins inhibit colon cancer proliferation and metastasis by triggering gut-lung axis immunotherapy

EMX2OS targeting IGF2BP1 represses Wilms’ tumour stemness, epithelial–mesenchymal transition and metastasis

AKT/GSK-3beta/VEGF signaling is involved in P2RY2 activation-induced the proliferation and metastasis of gastric cancer

Discovery of a Novel Bloom’s Syndrome Protein (BLM) Inhibitor Suppressing Growth and Metastasis of Prostate Cancer

DLGAP4 acts as an effective prognostic predictor for hepatocellular carcinoma and is closely related to tumour progression

Transmembrane protein 121 as a novel inhibitor of cervical cancer metastasis

Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer

High-Calcium Microenvironment during the Development of Kidney Calculi Can Promote Phenotypic Transformation of NRK-52E Cells by Inhibiting the Expression of Stromal Interaction Molecule-1

The TL1A-DR3 Axis in Asthma: Membrane-Bound and Secreted TL1A Co-Determined the Development of Airway Remodeling

Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter

Genetically encoded probiotic EcN 1917 alleviates alcohol-induced acute liver injury and restore gut microbiota homeostasis

Iodothyronine deiodinase 2 (DiO2) regulates trophoblast cell line cycle, invasion and apoptosis; and its downregulation is associated with early recurrent miscarriage

MicroRNA‑181a promotes epithelial‑mesenchymal transition in esophageal squamous cell carcinoma via the TGF‑β/Smad pathway

LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer

Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake

Tumor-associated macrophages promote prostate cancer progression via exosome-mediated miR-95 transfer

Sohlh2 alleviates malignancy of EOC cells under hypoxia via inhibiting the HIF1α/CA9 signaling pathway

MDM2 promotes epithelial–mesenchymal transition through activation of Smad2/3 signaling pathway in lung adenocarcinoma

MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis

Ski-related novel protein suppresses the development of diabetic nephropathy by modulating transforming growth factor-β signaling and microRNA-21 expression

Prognostic and clinicopathologic significance of AEG-1/MTDH and E-cadherin expression in human gallbladder carcinoma

The protective role of intermedin in promoting angiogenesis during renal fibrosis

Interleukin-27 Inhibits Trophoblast Cell Invasion and Migration by Affecting the Epithelial–Mesenchymal Transition in Preeclampsia

Novel HDAC5-interacting motifs of Tbx3 are essential for the suppression of E-cadherin expression and for the promotion of metastasis in hepatocellular carcinoma

Identification and differentiation therapy strategy of pterygium in vitro

Oral exposure of low-dose bisphenol A promotes proliferation of dorsolateral prostate and induces epithelial–mesenchymal transition in aged rats

MiR-181a contributes gefitinib resistance in non-small cell lung cancer cells by targeting GAS7

Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage

High Uric Acid-Induced Epithelial-Mesenchymal Transition of Renal Tubular Epithelial Cells via the TLR4/NF-kB Signaling Pathway

lncRNA H19 regulates epithelial–mesenchymal transition and metastasis of bladder cancer by miR-29b-3p as competing endogenous RNA

MFG-E8 overexpression promotes colorectal cancer progression via AKT/MMPs signalling

Silencing of ATPase family AAA domain-containing protein 2 inhibits migration and invasion of colorectal cancer cells.

Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathway

Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial–mesenchymal transition via NF-κB activation

Overexpression of SDF-1 activates the NF-κB pathway to induce epithelial to mesenchymal transition and cancer stem cell-like phenotypes of breast cancer cells

Regulator of cullins-1 expression knockdown suppresses the malignant progression of muscle-invasive transitional cell carcinoma by regulating mTOR/DEPTOR pathway

Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells

Expression of RKIP, E-cadherin and NF-kB p65 in esophageal squamous cell carcinoma and their correlations

RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells

Assessment of Transfection of AdCMV-EGFP to Rat Submandibular Gland Cells