Product Info Summary
SKU: | A01190-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CDC7 Kinase Antibody
SKU/Catalog Number
A01190-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CDC7 Kinase Antibody catalog # A01190-2. Tested in WB,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC7 Kinase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01190-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CDC7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01190-2 is reactive to CDC7 in Human, Mouse, Rat
Applications
A01190-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
63.888kDa
Background of CDC7
Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
Sato N., EMBO J. 16:4340-4351(1997).
Hess G.F., Gene 211:133-140(1998).
Jiang W., Proc. Natl. Acad. Sci. U.S.A. 94:14320-14325(1997).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Flow Cytometry validation of CDC7 using Anti-CDC7 Kinase Antibody (A01190-2).
Flow cytometric analysis of A431 cells with CDC7 Kinase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Click image to see more details
Figure 1. Western blotting validation for Anti-CDC7 Kinase Antibody A01190-2
Western blot analysis of CDC7 Kinase on different lysates using anti-CDC7 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For CDC7 (Source: Uniprot.org, NCBI)
Gene Name
CDC7
Full Name
Cell division cycle 7-related protein kinase
Weight
63.888kDa
Superfamily
protein kinase superfamily
Alternative Names
CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; CDC7 cell division cycle 7 (S. cerevisiae); CDC7L1MGC117361; CDC7-related kinase; cell division cycle 7 (S. cerevisiae); cell division cycle 7 homolog (S. cerevisiae); cell division cycle 7-like protein 1; cell division cycle 7-related protein kinase; EC 2.7.11.1; HsCDC7; Hsk1; huCdc7; MGC126237; MGC126238 CDC7 CDC7L1, HsCDC7, Hsk1, huCDC7 cell division cycle 7 cell division cycle 7-related protein kinase|CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1|CDC7-related kinase|cell division cycle 7 homolog|cell division cycle 7-like protein 1|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC7, check out the CDC7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDC7 Kinase Antibody (A01190-2)
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No publications found for A01190-2
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