|Reactivity||Human, Mouse, Rat|
|Ig Isotype||Mouse IgG2a|
|Product Name||Anti-CDK4 Antibody (Monoclonal, DCS-31)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for CDK4, cyclin-dependent kinase 4 (CDK4) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-CDK4 Antibody (Monoclonal, DCS-31) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1020)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Recombinant human Cdk4 protein.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||D(3) dopamine receptor|
|Alternative Names||D(3) dopamine receptor;Dopamine D3 receptor;DRD3;|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells.|
|Molecular Weight||44225 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. .|
|Research Areas||Neuroscience, Neurotransmission, Receptors / Channels
*You can search these to find other products in these research areas.
|Background||Cyclin-dependent kinase-4(CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.|
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (MA1020)
Promotion:Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.