Anti-CDK4 Antibody (Monoclonal, DCS-31)
Mouse IgG monoclonal antibody for CDK4, cyclin-dependent kinase 4 (CDK4) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins.
|Reactivity||Human, Mouse, Rat|
|Ig Isotype||Mouse IgG2a|
|Product Name||Anti-CDK4 Antibody (Monoclonal, DCS-31)
See all DRD3 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for CDK4 detection. Host: Mouse.Size: 100μg/vial. Tested applications: ICC. Reactive species: Human. CDK4 information: Molecular Weight: 44225 MW; Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells; Tissue Specificity: Brain.|
|Cite This Product||Anti-CDK4 Antibody (Monoclonal, DCS-31) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1020)|
|Specificity||Anti-CDK4 Antibody (Monoclonal, DCS-31) (MA1020) reacts with Human, Mouse, Rat DRD3, in native form and recombinant. Superfamily members of DRD3 are not reactive to MA1020.|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Immunogen||Recombinant human Cdk4 protein.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-CDK4 Antibody (Monoclonal, DCS-31) covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-CDK4 Antibody (Monoclonal, DCS-31) (MA1020).Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||D(3) dopamine receptor|
|Alternative Names||D(3) dopamine receptor;Dopamine D3 receptor;DRD3;|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells.|
|Molecular Weight||44225 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. .|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||Cyclin-dependent kinase-4(CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.|
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]