|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-CDK4 Antibody (Monoclonal, DCS-31)|
|Description||Mouse IgG monoclonal antibody for CDK4, cyclin-dependent kinase 4 (CDK4) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-CDK4 Antibody (Monoclonal, DCS-31) DCS-31 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1020)|
|Replacement Item||This antibody may replace the following items: sc-601|sc-23896|sc-260|sc-56361|sc-749|sc-56277|sc-53636|sc-166373|sc-136241|sc-70831|sc-70832|sc-393653|sc-601-G|sc-260-G|sc-393653-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant human Cdk4 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclin-dependent kinase 4|
|Molecular Weight||44225 MW|
|Protein Function||Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells.|
|Alternative Names||D(3) dopamine receptor;Dopamine D3 receptor;DRD3;|
|Research Areas|||cell biology|cell cycle|kinases/phosphatases|cdks| epigenetics and nuclear signaling| cancer| isotype/loading controls|cdk4||
Background for Cyclin-dependent kinase 4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CDK4 Antibody (Monoclonal, DCS-31) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -|
Western blot, 0.5-1μg/ml, Human, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.