Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31)

Boster Bio Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31) catalog # MA1020. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 12 publication(s).

Product Info Summary

SKU: MA1020
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: ICC, WB

Product Name

Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31)

See all Dopamine D3R/DRD3 products

SKU/Catalog Number

MA1020

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31) catalog # MA1020. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1020)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DCS-31

Isotype

Mouse IgG2a

Immunogen

Recombinant human Cdk4 protein.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1020 is reactive to DRD3 in Human, Mouse, Rat

Applications

MA1020 is guaranteed for ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat

Validation Images & Assay Conditions

Gene/Protein Information For DRD3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P35462

Gene ID

1814

Gene Name

DRD3

Full Name

D(3) dopamine receptor

Weight

44.225kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

D(3) dopamine receptor; D3DR; Dopamine D3 R; Dopamine D3 receptor; Dopamine D3R; dopamine receptor D3; DRD3; essential tremor 1; ETM1; FET1; MGC149204; MGC149205 DRD3 D3DR, ETM1, FET1 dopamine receptor D3 D(3) dopamine receptor|essential tremor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DRD3, check out the DRD3 Infographic

DRD3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DRD3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1020 has been cited in 12 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
MA1020 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000

Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells

Liao S, Xiao S, Chen H, Zhang M, Chen Z, Long Y, Gao L, He J, Ge Y, Yi W, Wu M, Li G, Zhou Y. Int J Oncol. 2017 Nov;51(5):1497-1507. doi: 10.3892/ijo.2017.4137. Epub 2017 Sep 27. The receptor for activated protein kinase C promotes cell growth, in...

Liu W, Dai Q, Lu N, Wei L, Ha J, Rong J, Mu R, You Q, Li Z, Guo Q. Biochem Cell Biol. 2011 Jun;89(3):287-98. Doi: 10.1139/O10-162. Epub 2011 Apr 14. Lyg-202 Inhibits The Proliferation Of Human Colorectal Carcinoma Hct-116 Cells Through Induction O...

Wang Hy, Huang Rp, Han P, Xue Db, Li Hb, Liu B, Shan P, Wang Qs, Li Ks, Li Hl. Cell Biochem Funct. 2014 Mar;32(2):201-8. Doi: 10.1002/Cbf.2995. Epub 2013 Sep 17. The Effects Of Artemisinin On The Proliferation And Apoptosis Of Vascular Smooth Musc...

Li Bh, Xu Sb, Li F, Zou Xg, Saimaiti A, Simayi D, Wang Yh, Zhang Y, Yuan J, Zhang Wj. Cell Signal. 2012 Mar;24(3):718-25. Doi: 10.1016/J.Cellsig.2011.11.005. Epub 2011 Nov 13. Stat6 Activity-Related Th2 Cytokine Profile And Tumor Growth Advantage ...

Zhang H, Zhao B, Huang C, Meng Xm, Bian Eb, Li J. Plos One. 2014 May 2;9(5):E95520. Doi: 10.1371/Journal.Pone.0095520. Ecollection 2014. Melittin Restores Pten Expression By Down-Regulating Hdac2 In Human Hepatocelluar Carcinoma Hepg2 Cells.

Han W, Liu Gn. Life Sci. 2010 Feb 13;86(7-8):234-43. Doi: 10.1016/J.Lfs.2009.12.005. Epub 2009 Dec 16. Egr-1 Decoy Odns Inhibit Vascular Smooth Muscle Cell Proliferation And Neointimal Hyperplasia Of Balloon-Injured Arteries In Rat.

Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.

Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.

Cai K, Xu J, Zhang Y. Neurosci Lett. 2012 Jul 26;522(1):62-6. Doi: 10.1016/J.Neulet.2012.06.017. Epub 2012 Jun 15. Bone Marrow Stromal Cells Induce Cell Cycle Arrest In Reactive Astrocytes In Vitro.

Li B, Xu Yj, Chu Xm, Gao Mh, Wang Xh, Nie Sm, Yang F, Lv Cy. Immunol Lett. 2013 Nov-Dec;156(1-2):68-81. Doi: 10.1016/J.Imlet.2013.09.025. Epub 2013 Sep 29. Molecular Mechanism Of Inhibitory Effects Of Cd59 Gene On Atherosclerosis In Apoe (-/-) Mice.

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15 Customer Q&As for Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31)

Question

Will anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 work for ICC with right ovary?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

According to the expression profile of right ovary, CDK4 is highly expressed in right ovary. So, it is likely that anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 will work for ICC with right ovary.

Boster Scientific Support

Answered: 2020-05-06

Question

We were well pleased with the WB result of your anti-CDK4 antibody (Monoclonal, DCS-31). However we have been able to see positive staining in muscle cytoplasm. using this antibody. Is that expected? Could you tell me where is CDK4 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

According to literature, muscle does express CDK4. Generally CDK4 expresses in cytoplasm. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-04-23

Question

Do you have a BSA free version of anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 available?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-13

Question

Can you help my question with product MA1020, anti-CDK4 antibody (Monoclonal, DCS-31). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1020 anti-CDK4 antibody (Monoclonal, DCS-31), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-03

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-CDK4 antibody (Monoclonal, DCS-31) MA1020. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-10

Question

Is a blocking peptide available for product anti-CDK4 antibody (Monoclonal, DCS-31) (MA1020)?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

We do provide the blocking peptide for product anti-CDK4 antibody (Monoclonal, DCS-31) (MA1020). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-17

Question

Is this MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) reactive to the isotypes of CDK4?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The immunogen of MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) is Recombinant human Cdk4 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-15

Question

We are currently using anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-31

Answer

The anti-CDK4 antibody (Monoclonal, DCS-31) (MA1020) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-31

Question

We purchased anti-CDK4 antibody (Monoclonal, DCS-31) for ICC on embryo a few years ago. I am using rat, and We intend to use the antibody for WB next. I am interested in examining embryo as well as right ovary in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

I took a look at the website and datasheets of our anti-CDK4 antibody (Monoclonal, DCS-31) and I see that MA1020 has been validated on rat in both ICC and WB. Thus MA1020 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-25

Question

I was wanting to use your anti-CDK4 antibody (Monoclonal, DCS-31) for ICC for rat right ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat right ovary identification?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

As indicated on the product datasheet, MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) has been tested for ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right ovary in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-17

Question

Does MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Huang

Verified customer

Asked: 2019-02-22

Answer

It shows on the product datasheet, MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-02-22

Question

We have observed staining in mouse right ovary. What should we do? Is anti-CDK4 antibody (Monoclonal, DCS-31) supposed to stain right ovary positively?

Verified Customer

Verified customer

Asked: 2018-05-11

Answer

From literature right ovary does express CDK4. From Uniprot.org, CDK4 is expressed in right ovary, embryo, muscle, among other tissues. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-05-11

Question

We are interested in to test anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 on rat right ovary for research purposes, then I may be interested in using anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-12-18

Answer

The products we sell, including anti-CDK4 antibody (Monoclonal, DCS-31) MA1020, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-12-18

Question

Here is the WB image, lot number and protocol we used for right ovary using anti-CDK4 antibody (Monoclonal, DCS-31) MA1020. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-06-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-02

Question

I see that the anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 works with ICC, what is the protocol used to produce the result images on the product page?

K. Patel

Verified customer

Asked: 2016-05-24

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-05-24

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