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- Table of Contents
Cyclin-dependent kinase 4 Antibodies
AND CDK4 ELISA kits, Cyclin-dependent kinase 4 Proteins
Many biological assays use antibodies to detect Cyclin-dependent kinase 4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Cyclin-dependent kinase 4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Cyclin-dependent kinase 4 antibodies...
Designing a Western blot for Cyclin-dependent kinase 4? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CDK4 Western Blot GuideCDK4 Infographic by Boster Bio
All CDK4 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
Flow Cytometry, IF, ICC, WB
$370
Cat # PA1428-1
100 μg/vial
Cat # PA1428-1
100 μg/vial
Anti-CDK4 Rabbit Monoclonal Antibody
2 Citations
Human, Mouse, Rat
IP, IF, ICC, WB
$370
Cat # M00159
100 μl
Cat # M00159
100 μl
Human
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # M00159-1
100 μl
Cat # M00159-1
100 μl
Human, Mouse, Rat
ELISA, IF, IHC-P, WB
$481
Cat # A00016-1
0.1 mg
Cat # A00016-1
0.1 mg
Human, Mouse, Rat
ELISA, IP, IF, IHC, WB
$146
Cat # M00159-3
20μL, 40μL, 100μL, 200μL
Cat # M00159-3
20μL, 40μL, 100μL, 200μL
Human
Flow Cytometry, IF, IHC, ICC, WB
$405
Cat # A00159-1
100ul
Cat # A00159-1
100ul
Human, Mouse, Rat
IP, IF, ICC, WB
$405
Cat # A00159
100ul
Cat # A00159
100ul
CDK4 Overview
Facts about Cyclin-dependent kinase 4.
Cyclin-dependent kinase 4 (CDK4)
Ser/Thr-kinase part of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase.
Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are important integrators of various mitogenenic and antimitogenic signals.
Gene Information
| Human | |
|---|---|
| Gene Name: | CDK4 |
| Uniprot: | P11802 |
| Entrez: | 1019 |
Family
| Belongs to: |
|---|
| protein kinase superfamily |
Alternative Names
CDK4; Cell division protein kinase 4; CMM3; cyclin-dependent kinase 4; EC 2.7.11; EC 2.7.11.22; melanoma cutaneous malignant, 3; MGC14458; PSK-J3; PSK-J3cell division kinase 4
Molecular Weight
Mass (kDA):
33.73 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 12q14.1 |
| Sequence: | 12; NC_000012.12 (57747727..57752310, complement) |
Subcellular Localizations
Cytoplasm. Nucleus. Nucleus membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
Diseases
- Neoplasms
- Malignant Neoplasms
- Retinoblastoma
- Carcinoma
- Cell Transformation, Neoplastic
- Malignant Paraganglionic Neoplasm
- Melanoma
- Malignant Neoplasm Of Breast
- Mammary Neoplasms
- Growth Arrest
- Skin Neoplasms
- Leukemia
Interacting Proteins
- CCND1
- CCND2
- CCND3
- CDC37
- CDK7
- CDKN1A
- CDKN1B
- CDKN2A
- CDKN2B
- CDKN2C
Pathways
- Cell Cycle
- Cell Proliferation
- Cell Cycle Arrest
- G1 Phase
- Cell Growth
- S Phase
- Cell Death
- Pathogenesis
- Localization
- Senescence
- Cell Division
- Induction Of Apoptosis
- Hyperphosphorylation
PTMs
- Phosphorylation
- Cleavage
- Methylation
- Dephosphorylation
- Acetylation
- Ubiquitination
- Reduction
- Demethylation
- Oxidation
- Biotinylation
Research Areas
- Cancer
- Cell Cycle and Replication
- Core ESC-Like Genes
- mTOR Pathway
- Stem Cell Markers
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CDK4
bosterbio.com/bosterbio-gene-info-cards/CDK4
References
PMID: 9192850 by Elkahloun A.G., et al. Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers.
PMID: 7652577 by Wolfel T., et al. A p16INK4a-insensitive CDK4 mutant targeted by cytolytic T lymphocytes in a human melanoma.
24 Publications of Boster Cyclin-dependent kinase 4 Antibodies & ELISA Kits
- PubMed ID: 10.7150/ijms.3859, Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric...
- PubMed ID: 10.3892/or.2016.4935, Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastati...
- PubMed ID: 10.3389/fonc.2021.648985, Combined Anti-Cancer Effects of Platycodin D and Sorafenib on Androgen-Independe...
- PubMed ID: 10.1038/s41419-020-2238-1, P53 and Parkin co-regulate mitophagy in bone marrow mesenchymal stem cells to pr...
- PubMed ID: 10.1016/j.neulet.2009.09.052, Dynamic changes of cell cycle elements in the ischemic brain after bone marrow s...
- PubMed ID: 10.1016/j.mce.2020.111005, Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulatin...
- PubMed ID: 10.1002/cbin.10892, OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest ...
- PubMed ID: -, Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlyi...
- PubMed ID: 15378766, Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell l...
- PubMed ID: 30070315, Expression of p16 protein in infantile hemangioma
- PubMed ID: 22359486, Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric...
- PubMed ID: 4576245, Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell l...
- PubMed ID: 22228178, Song Lj, Liu Rj, Zeng Z, Alper Sl, Cui Hj, Lu Y, Zheng L, Yan Zw, Fu Gh. J Mol M...
- PubMed ID: 24076388, Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Gu...
- PubMed ID: 21491996, Liu W, Dai Q, Lu N, Wei L, Ha J, Rong J, Mu R, You Q, Li Z, Guo Q. Biochem Cell ...
- PubMed ID: 24105880, Wang Hy, Huang Rp, Han P, Xue Db, Li Hb, Liu B, Shan P, Wang Qs, Li Ks, Li Hl. C...
- PubMed ID: 22108090, Li Bh, Xu Sb, Li F, Zou Xg, Saimaiti A, Simayi D, Wang Yh, Zhang Y, Yuan J, Zhan...
- PubMed ID: 24788349, Zhang H, Zhao B, Huang C, Meng Xm, Bian Eb, Li J. Plos One. 2014 May 2;9(5):E955...
- PubMed ID: 20025889, Han W, Liu Gn. Life Sci. 2010 Feb 13;86(7-8):234-43. Doi: 10.1016/J.Lfs.2009.12....
- PubMed ID: 25734831, Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599...
- PubMed ID: 25431082, Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug ...
*Showing only the more recent 20. More publications can be found for each product on its corresponding product page