Anti-Cyclin-dependent kinase 9 Cdk9 Antibody

Boster Bio Anti-Cyclin-dependent kinase 9 Cdk9 Antibody catalog # A00794. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A00794
Size: 100μL
Reactive Species: Human
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-Cyclin-dependent kinase 9 Cdk9 Antibody

See all CDK9 products

SKU/Catalog Number

A00794

Size

100μL

Form

Liquid

Description

Boster Bio Anti-Cyclin-dependent kinase 9 Cdk9 Antibody catalog # A00794. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Cite This Product

Anti-Cyclin-dependent kinase 9 Cdk9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00794)

Host

Rabbit

Clonality

Polyclonal

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00794 is reactive to CDK9 in Human

Applications

A00794 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:3, 000, human, mouse, rat, rat and mouse
Direct ELISA, 1:10, 000 - 1:50, 000, human, mouse, rat, rat and mouse
ImmunoPrecipitation: 1:100, human, mouse, rat, rat and mouse
Immunohistochemistry: 1:200 - 1:1, 000, human, mouse, rat, rat and mouse

Validation Images & Assay Conditions

Gene/Protein Information For CDK9 (Source: Uniprot.Org, NCBI)

Uniprot ID

P50750

Gene ID

1025

Gene Name

CDK9

Full Name

Cyclin-dependent kinase 9

Weight

42.778kDa

Superfamily

protein kinase superfamily

Alternative Names

C-2k; CDC2L4CTK1; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); cyclin-dependent kinase 9; EC 2.7.11; EC 2.7.11.22; EC 2.7.11.23; PITALRE; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK CDK9 C-2k, CDC2L4, CTK1, PITALRE, TAK cyclin dependent kinase 9 cyclin-dependent kinase 9|CDC2-related kinase|cell division cycle 2-like protein kinase 4|cell division protein kinase 9|serine/threonine protein kinase PITALRE|tat-associated kinase complex catalytic subunit

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDK9, check out the CDK9 Infographic

CDK9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDK9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00794

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Cyclin-dependent kinase 9 Cdk9 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Cyclin-dependent kinase 9 Cdk9 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-Cyclin-dependent kinase 9 Cdk9 Antibody

Question

We have observed staining in human liver. Are there any suggestions? Is anti-Cdk9 antibody supposed to stain liver positively?

L. Li

Verified customer

Asked: 2020-02-10

Answer

Based on literature liver does express CDK9. Based on Uniprot.org, CDK9 is expressed in right ovary, cervix, cervix carcinoma, liver, among other tissues. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2020-02-10

Question

Is a blocking peptide available for product anti-Cdk9 antibody (A00794)?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

We do provide the blocking peptide for product anti-Cdk9 antibody (A00794). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-15

Question

We need to test anti-Cdk9 antibody A00794 on human liver for research purposes, then I may be interested in using anti-Cdk9 antibody A00794 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

The products we sell, including anti-Cdk9 antibody A00794, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-10

Question

Would anti-Cdk9 antibody A00794 work for IHC with liver?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

According to the expression profile of liver, CDK9 is highly expressed in liver. So, it is likely that anti-Cdk9 antibody A00794 will work for IHC with liver.

Boster Scientific Support

Answered: 2019-11-28

Question

We are currently using anti-Cdk9 antibody A00794 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

The anti-Cdk9 antibody (A00794) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-05

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Cdk9 antibody A00794. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-12

Question

We have tried in the past anti-Cdk9 antibody for WB on cervix a few months ago. I am using human, and We want to use the antibody for IP next. My lab would like examining cervix as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

I looked at the website and datasheets of our anti-Cdk9 antibody and it appears that A00794 has been validated on human in both WB and IP. Thus A00794 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-10

Question

Our lab want to know about using your anti-Cdk9 antibody for positive regulation of cardiac muscle hypertrophy studies. Has this antibody been tested with western blotting on mouse tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

We appreciate your inquiry. This A00794 anti-Cdk9 antibody is validated on mouse tissue. It is guaranteed to work for IP, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-18

Question

My question regarding product A00794, anti-Cdk9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00794 anti-Cdk9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-21

Question

My colleagues were well pleased with the WB result of your anti-Cdk9 antibody. However we have been able to see positive staining in liver pml body. using this antibody. Is that expected? Could you tell me where is CDK9 supposed to be expressed?

L. Brown

Verified customer

Asked: 2019-05-13

Answer

From literature, liver does express CDK9. Generally CDK9 expresses in nucleus. cytoplasm. nucleus, pml body. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-05-13

Question

I see that the anti-Cdk9 antibody A00794 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-10-23

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-10-23

Question

Here is the WB image, lot number and protocol we used for liver using anti-Cdk9 antibody A00794. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-06-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-27

Question

Does A00794 anti-Cdk9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Singh

Verified customer

Asked: 2015-05-28

Answer

As indicated on the product datasheet, A00794 anti-Cdk9 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-05-28

Question

Is there a BSA free version of anti-Cdk9 antibody A00794 available?

T. Li

Verified customer

Asked: 2015-05-06

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cdk9 antibody A00794 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-05-06

Question

I was wanting to use your anti-Cdk9 antibody for IHC for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?

N. Huang

Verified customer

Asked: 2015-02-18

Answer

It shows on the product datasheet, A00794 anti-Cdk9 antibody has been tested for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-02-18

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00794
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.