Anti-CENP-E Monoclonal Antibody

Product Name

Anti-CENP-E Monoclonal Antibody

View all CENPE Antibodies

SKU/Catalog Number

M04553

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-CENP-E Monoclonal Antibody (Catalog # M04553). Tested in IF, IP, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-CENP-E Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04553)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 1H12 IgG1

Isotype

IgG1

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.

Reactive Species

M04553 is reactive to CENPE in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

316.4 kDa

Background of CENPE

CENP-E, Centrosome-associated protein E, is a kinesin-like minus-end directed microtubule motor protein that accumulates in the G₂ phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENP-E is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. CENP-E interacts with CENP-F and BUBR1 kinase. CENP-E associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04553 is guaranteed for ELISA, IP, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IF Microscopy: 1:500 - 1:2,000
IP: 1:200
WB: 1:500 - 1:2,000
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 320 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information CENPE

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