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Product Info Summary
| SKU: | A09176-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Cerebellin 1 CBLN1 Antibody
SKU/Catalog Number
A09176-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cerebellin 1 CBLN1 Antibody catalog # A09176-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cerebellin 1 CBLN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09176-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CBLN1. AA range:131-180
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09176-1 is reactive to CBLN1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
21.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09176-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using Cerebellin 1 Polyclonal antibody.
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NEXMIF overexpression results in transcriptional dysregulation in the brain. (A) RNA sequencing bar chart showing the normalized (Log2) fold changes of the top 44 of 161 total differentially expressed genes (DEGs) in the hippocampus (HPC) of P70 NEX OX mice. In total, 131 DEGs (81%) were downregulated and 30 DEGs (19%) were upregulated by at least a 1.3-fold change in the NEX OX mouse brain, relative to CTRL. (B) Gene Ontology (GO) enrichment analysis bar chart depicting the most enriched biological processes. The “# of DEGs” refers to the number of genes from the RNA sequencing dataset within each predefined Mus musculus GO biological process. Each bar reflects the percentage of upregulated (red) and/or downregulated (green) DEGs within each GO biological process. The False Discovery Rate (FDR) is a statistical measure of true nulls. For example, an FDR of 3% means that, among all genes considered significant, 3% of these genes are truly null. (C) Selected list of top hippocampal DEGs indicating their associated GO term, fold change in expression (NEX OX / CTRL), and adjusted p value. (D) Western blot showing the protein expression of CBLN1 (top panel), BAIAP3 (middle panel), and SGK1 (bottom panel) in the brain lysates of P70 CTRL and NEX OX mice. (E) Quantification of the western blots in (D) showed a significant reduction in CBLN1 and BAIAP3 protein in P70 NEX OX brains, in addition to an increase in SGK1 protein, consistent with RNA sequencing data. N = 3–4 mice/group. Data are represented as average ± SEM. Two-tailed student’s t test (E) : * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
Index in PubMed under a CC BY license. PMID: 40606839
Specific Publications For Anti-Cerebellin 1 CBLN1 Antibody (A09176-1)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Cerebellin 1 CBLN1 Antibody
Question
See attached the WB image, lot number and protocol we used for cerebellum using anti-Cerebellin 1 antibody A09176-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-30
Question
I was wanting to use your anti-Cerebellin 1 antibody for WB for rat cerebellum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cerebellum identification?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
You can see on the product datasheet, A09176-1 anti-Cerebellin 1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-14
Question
My question regarding product A09176-1, anti-Cerebellin 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09176-1 anti-Cerebellin 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-30
Question
We are currently using anti-Cerebellin 1 antibody A09176-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
The anti-Cerebellin 1 antibody (A09176-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-10
Question
Would anti-Cerebellin 1 antibody A09176-1 work for WB with cerebellum?
F. Krishna
Verified customer
Asked: 2017-08-11
Answer
According to the expression profile of cerebellum, CBLN1 is highly expressed in cerebellum. So, it is likely that anti-Cerebellin 1 antibody A09176-1 will work for WB with cerebellum.
Boster Scientific Support
Answered: 2017-08-11
Question
I see that the anti-Cerebellin 1 antibody A09176-1 works with WB, what is the protocol used to produce the result images on the product page?
D. Johnson
Verified customer
Asked: 2016-12-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-12-30
