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Product Info Summary
| SKU: | A13892 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Kelch-like protein 15 KLHL15 Antibody
SKU/Catalog Number
A13892
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Kelch-like protein 15 KLHL15 Antibody (Catalog # A13892). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KLHL15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Kelch-like protein 15 KLHL15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13892)
Host
Rabbit
Contents
KLHL15 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KLHL15 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human KLHL15. The immunogen is located within the last 50 amino acids of KLHL15.
Reactive Species
A13892 is reactive to KLHL15 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
69.8 kDa
Background of KLHL15
KLHL15 is a member of a growing superfamily of kelch repeat-containing proteins. The kelch motif was discovered in the sequence of the Drosophila kelch ORF1 protein and forms a conserved beta-propeller tertiary structure. These kelch-like proteins have been implicated in embryogenesis and carcinogenesis through cytoskeleton organization. KLHL15 mRNA is widely expressed in many tissues and is thought to be involved in protein ubiquination and cytoskeleton organization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13892 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KLHL15 antibody can be used for detection of KLHL15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KLHL15 in HeLa cell lysate with KLHL15 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of KLHL15 in HeLa cells with KLHL15 antibody at 20 μg/mL.
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Immunocytochemistry of KLHL15 in HeLa cells with KLHL15 antibody at 5 μg/mL.
Specific Publications For Anti-Kelch-like protein 15 KLHL15 Antibody (A13892)
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