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Product Info Summary
| SKU: | A00028 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CFTR Antibody
SKU/Catalog Number
A00028
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CFTR Antibody (Catalog # A00028). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CFTR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-CFTR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00028)
Host
Rabbit
Contents
CFTR Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CFTR antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CFTR. The immunogen is located within amino acids 1290 - 1340 of CFTR.
Cross-reactivity
CFTR antibody is human, mouse and rat reactive.
Reactive Species
A00028 is reactive to CFTR in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
168.1 kDa
Background of CFTR
The cystic fibrosis transmembrane conductance regulator (CFTR) protein is a member of the ATP-binding cassette (ABC) transporter superfamily, and a member of the MRP subfamily that is involved in multi-drug resistance (1,2). CFTR functions as a chloride channel and controls the regulation of other transport pathways (3). Mutations in this gene are associated with the autosomal recessive disorder cystic fibrosis, the most common, fatal, inherited disease of caucasian populations (1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00028 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CFTR antibody can be used for detection of CFTR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CFTR in human small intestine tissue lysate with CFTR antibody at 1 μg/ml.
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Immunohistochemistry of CFTR in human small intestine tissue with CFTR antibody at 5 μg/mL.
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Immunofluorescence of CFTR in human small intestine tissue with CFTR antibody at 20 μg/mL.
Specific Publications For Anti-CFTR Antibody (A00028)
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