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Product Info Summary
| SKU: | M04245-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Coronin 1a (CORO1A) Mouse Monoclonal Antibody [Clone ID: OTI1A5]
SKU/Catalog Number
M04245-1
Size
100 µl
Description
Boster Bio CORO1A (Coronin 1a) mouse monoclonal antibody, clone OTI1A5 (formerly 1A5). Catalog# M04245-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Coronin 1a (CORO1A) Mouse Monoclonal Antibody [Clone ID: OTI1A5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04245-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1A5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CORO1A (NP_009005) produced in HEK293T cell.
Reactive Species
M04245-1 is reactive to CORO1A in Human, Mouse, Rat
Observed Molecular Weight
50.8 kDa
Calculated molecular weight
51.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04245-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
IHC 1:50
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CORO1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CORO1A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CORO1A monoclonal antibody.
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HEK293T cells transfected with either CORO1A (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CORO1A antibody (M04245-1)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-CORO1A mouse monoclonal antibody (M04245-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CORO1A.
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IHC analysis of CORO1A using anti-CORO1A antibody (M04245-1).
CORO1A was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CORO1A Antibody (M04245-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of CORO1A using anti-CORO1A antibody (M04245-1).
CORO1A was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CORO1A Antibody (M04245-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of CORO1A using anti-CORO1A antibody (M04245-1).
CORO1A was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CORO1A Antibody (M04245-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Coronin 1a (CORO1A) Mouse Monoclonal Antibody [Clone ID: OTI1A5] (M04245-1)
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