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Product Info Summary
| SKU: | A00164-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-IL17C Antibody Picoband®
SKU/Catalog Number
A00164-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL17C Antibody Picoband® catalog # A00164-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL17C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00164-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL17C recombinant protein (Position: H15-Q194).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00164-3 is reactive to Il17c in Mouse, Rat
Observed Molecular Weight
20 kDa
Calculated molecular weight
21.6 kDa
Background of Il17c
IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00164-3 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
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Western blot analysis of IL17C using anti-IL17C antibody (A00164-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse IL17C protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17C antigen affinity purified polyclonal antibody (Catalog # A00164-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17C at approximately 20KD. The expected band size for IL17C is at 20KD.
Specific Publications For Anti-IL17C Antibody Picoband® (A00164-3)
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1 Customer Q&As for Anti-IL17C Antibody Picoband®
Question
We are currently using anti-IL17C antibody A00164-3 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on primate tissues as well?
V. Roberts
Verified customer
Asked: 2018-10-30
Answer
The anti-IL17C antibody (A00164-3) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-30
