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- Table of Contents
CPT1B antibody (PB9491) performs well in IF, IHC, IHC-F, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IF analysis of CPT1B using anti-CPT1B antibody (PB9491).
CPT1B was detected in a paraffin-embedded section of mouse cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CPT1B Antibody (PB9491) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CPT1B was detected in a paraffin-embedded section of mouse cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CPT1B Antibody (PB9491) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-CPT1B Antibody Picoband®
CPT1B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IF, IHC, IHC-F, WB |
Tested Applications
CPT1B antibody was positively detected by WB in:
rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CPT1B antibody was positively detected by IHC in:
Mouse Skeletal Muscle tissue, Rat Cardiac Muscle tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CPT1B antibody was positively detected by IHC-F in:
mouse cardiac muscle tissue, rat cardiac muscle tissue
CPT1B antibody was positively detected by IF in:
mouse cardiac tissue, rat cardiac tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunofluorescence
5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Human
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CPT1B Antibody Picoband®
View all CPT1B Antibodies
View all CPT1B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CPT1B, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
88 kDa
Calculated Molecular Weight
87.8 kDa
Background of CPT1B
CPT1B is located on 22q13.33. The protein encoded by this gene, a member of the carnitine/ choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene.
Cite This Product
Anti-CPT1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9491)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
97%
High Homology
Mouse
93%
High Homology
Monkey
93%
High Homology
Rat
87%
Moderate Homology
Cow
87%
Moderate Homology
Sheep
87%
Moderate Homology
Zebrafish
60%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CPT1B antibody
Immunohistochemistry (IHC) protocol for CPT1B antibody
Immunofluorescence (IF) protocol for CPT1B antibody
Publications
Application:
Species:
Perfluorooctanoic acid-induced exacerbation of myocardial ischemia-reperfusion injury: Roles of peroxisome proliferation activated receptor alpha-related fatty acid metabolism
Published: 2026-08-03
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 6.60
Species: Mouse
PMID: 42546566
miR-122/PPARβ axis is involved in hypoxic exercise and modulates fatty acid metabolism in skeletal muscle of obese rats
Published: 2024-02-20
Journal: Heliyon
Application: WB
Impact Factor: 3.40
Species: Rat
PMID: 38434053
An obesogenic diet impairs uncoupled substrate oxidation and promotes whitening of the brown adipose tissue in rats
Published: 2022-11-23
Journal: JOURNAL OF PHYSIOLOGY-LONDON
Impact Factor: 6.23
Species: Rat
PMID: 36419345
Therapeutic evaluation and metabolic reprograming of isosteviol sodium in a rat model of ischemic cardiomyopathy
Published: 2021-09-30
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.43
Species: Rat
PMID: 34599913
Docosahexaenoic and eicosapentaenoic fatty acids differentially regulate glucose and fatty acid metabolism in L6 rat skeletal muscle cells
Published: 2020-12-17
Journal: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
Application: WB
Impact Factor: 3.49
Species: Rat
PMID: 32966124
Astragaloside IV inhibits ventricular remodeling and improves fatty acid utilization in rats with chronic heart failure
Published: 2018-05-22
Journal: BIOSCIENCE REPORTS
Application: WB
Impact Factor: 2.90
Species: Rat
PMID: 29301869
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Product Promise
We promise Anti-CPT1B Antibody Picoband® performs excellently in relevant applications for IF, IHC, IHC-F, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9491
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026