Anti-CXCR2 Antibody

Boster Bio Anti-CXCR2 Antibody catalog # PA1029. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: PA1029
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-CXCR2 Antibody

See all CXCR2/IL-8RB products

SKU/Catalog Number

PA1029

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CXCR2 Antibody catalog # PA1029. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CXCR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1029)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human CXCR2 (196-212aa CYEDMGNNTANWRMLLR), different from the related mouse sequence by six amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1029 is reactive to CXCR2 in Human, Mouse, Rat

Applications

PA1029 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For CXCR2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P25025

Gene ID

3579

Gene Name

CXCR2

Full Name

C-X-C chemokine receptor type 2

Weight

40.759kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; CMKAR2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 RB; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B CXCR2 CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB C-X-C motif chemokine receptor 2 C-X-C chemokine receptor type 2|CXC-R2|CXCR-2|CXCR2 gene for IL8 receptor type B|GRO/MGSA receptor|IL-8 receptor type 2|IL-8R B|chemokine (CXC) receptor 2|high affinity interleukin-8 receptor B|interleukin 8 receptor type 2|interleukin 8 receptor, beta|interleukin-8 receptor type B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCR2, check out the CXCR2 Infographic

CXCR2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1029 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cui D,Zhao Y,Xu J.Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 pathways.Cancer Biol Ther.2019;20(5):608-616.doi:10.1080/15384047.2018.1539289.Epub 2018 Nov 7.PMID:30404567;PMCID:PMC6606038.
Species: Human,Mouse
PA1029 usage in article: APP:WB, SAMPLE:PTC CELL, DILUTION:1:200

Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 pathways Dong Cui 1, Yongfu Zhao 1, Jingchao Xu 1 Cancer Biol Ther. 2019;20(5):608-616. doi:

Overexpression of GRK6 attenuates neuropathic pain via suppression of CXCR2 in rat dorsal root ganglion

Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2

Xu J, Zhu Md, Zhang X, Tian H, Zhang Jh, Wu Xb, Gao Yj. J Neuroinflammation. 2014 Mar 1;11:38. Doi: 10.1186/1742-2094-11-38. Nf??b-Mediated Cxcl1 Production In Spinal Cord Astrocytes Contributes To The Maintenance Of Bone Cancer Pain In Mice.

Chen G, Park Ck, Xie Rg, Berta T, Nedergaard M, Ji Rr. Brain. 2014 Aug;137(Pt 8):2193-209. Doi: 10.1093/Brain/Awu140. Epub 2014 Jun 11. Connexin-43 Induces Chemokine Release From Spinal Cord Astrocytes To Maintain Late-Phase Neuropathic Pain In Mice.

Zhang Zj, Cao Dl, Zhang X, Ji Rr, Gao Yj. Pain. 2013 Oct;154(10):2185-97. Doi: 10.1016/J.Pain.2013.07.002. Epub 2013 Jul 4. Chemokine Contribution To Neuropathic Pain: Respective Induction Of Cxcl1 And Cxcr2 In Spinal Cord Astrocytes And Neurons.

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4 Customer Q&As for Anti-CXCR2 Antibody

Question

We are currently using anti-CXCR2 antibody PA1029 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

D. Banerjee

Verified customer

Asked: 2020-05-01

Answer

The anti-CXCR2 antibody (PA1029) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-05-01

Question

We have seen staining in mouse brain. Do you have any suggestions? Is anti-CXCR2 antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2018-04-25

Answer

Based on literature brain does express CXCR2. Based on Uniprot.org, CXCR2 is expressed in blood, placenta, brain, among other tissues. Regarding which tissues have CXCR2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 7929358

Boster Scientific Support

Answered: 2018-04-25

Question

I am looking for using your anti-CXCR2 antibody for positive regulation of angiogenesis studies. Has this antibody been tested with western blotting on kidney tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-01-26

Answer

I appreciate your inquiry. This PA1029 anti-CXCR2 antibody is tested on human rectal cancer tissue lysate, rat spleen tissue, kidney tissue, brain tissue, mouse testis tissue, hepa whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-01-26

Question

My team were well pleased with the WB result of your anti-CXCR2 antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is CXCR2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-12-14

Answer

According to literature, placenta does express CXCR2. Generally CXCR2 expresses in cell membrane. Regarding which tissues have CXCR2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 7929358

Boster Scientific Support

Answered: 2017-12-14

Size

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PA1029
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