SKU PA1029
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-CXCR2 Antibody
SKU/Catalog Number PA1029
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 2(CXCR2) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-CXCR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1029)
Host Rabbit
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human CXCR2(196-212aa CYEDMGNNTANWRMLLR), different from the related mouse sequence by six amino acids.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1029 1 WB anti cxcr2 il 8rb antibody.jpg

Anti-CXCR2 antibody, PA1029, Western blotting
Lane 1: Human Rectal Cancer Tissue Lysate
Lane 2: Human Rectal Cancer Tissue Lysate

/antibody/pa1029 2 WB anti cxcr2 il 8rb antibody.jpg

Anti- CXCR2 antibody, PA1029, Western blotting
All lanes: Anti CXCR2(PA1029) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Lane 6: Mouse BrainTissue Lysate at 50ug
Predicted bind size: 41KD
Observed bind size: 41KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P25025
Gene Name CXCR2
Protein Name C-X-C chemokine receptor type 2
Alternative Names C-X-C chemokine receptor type 2;CXC-R2;CXCR-2;CDw128b;GRO/MGSA receptor;High affinity interleukin-8 receptor B;IL-8R B;IL-8 receptor type 2;CD182;CXCR2;IL8RB;
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Molecular Weight 40759 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
Background CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family(subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.

Order Product (PA1029)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships in 5-7 business days.

Troubleshooting

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Publications

Chemokine contribution to neuropathic pain: respective induction of CXCL1 and CXCR2 in spinal cord astrocytes and neurons
Overexpression of GRK6 attenuates neuropathic pain via suppression of CXCR2 in rat dorsal root ganglion
Connexin-43 induces chemokine release from spinal cord astrocytes to maintain late-phase neuropathic pain in mice
Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2
NF?B-mediated CXCL1 production in spinal cord astrocytes contributes to the maintenance of bone cancer pain in mice
Zhang Zj, Cao Dl, Zhang X, Ji Rr, Gao Yj. Pain. 2013 Oct;154(10):2185-97. Doi: 10.1016/J.Pain.2013.07.002. Epub 2013 Jul 4. Chemokine Contribution To Neuropathic Pain: Respective Induction Of Cxcl1 And Cxcr2 In Spinal Cord Astrocytes And Neurons.

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.