Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 2(CXCR2) detection. Tested with WB in Human;Mouse;Rat. Cited in 5 publication(s).
Anti-CXCR2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-CXCR2 Antibody
See all CXCR2/IL-8RB primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for IL 8RB/CXCR2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. IL 8RB/CXCR2 information: Molecular Weight: 40759 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein.|
|Cite This Product||Anti-CXCR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1029)|
|Similar Products From
|Anti-CXCR2 Antibody may replace the following items: sc 22661|sc 30008|sc 32780|sc 682|sc 683|sc 7303|sc 7304|sc 988|sc 989.|
|Reactivity/Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human CXCR2(196-212aa CYEDMGNNTANWRMLLR), different from the related mouse sequence by six amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CXCR2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3579|
|Species Of This Entry||Human|
|Protein Name||C-X-C chemokine receptor type 2|
|Superfamily||G-protein coupled receptor 1 family|
|Alternative Names||CXCR2/IL-8RB|CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; CMKAR2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 RB; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Acetylation; Methylation; Dephosphorylation; Reduction; Oxidation; Formylation; Nitration; Glycosylation; Amidation; Citrullination|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Gene Location||2q35, on Chromasome 2, gene sequence: NC_000002.12 (218125294..218137253)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CXCR2 (Source: Uniprot.org, NCBI)
|Protein Function||Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.|
|Interacting Proteins||CXCL8; IQGAP1|
|Related Diseases||Inflammation; Neoplasms; Malignant Neoplasms; Tumor Angiogenesis; Tissue Adhesions; Pathologic Neovascularization; Neoplasm Metastasis; Inflammatory Response; Cholangiocarcinoma; Lung Diseases; Chronic Obstructive Airway Disease; Pneumonia; Melanoma; Lung Diseases, Obstructive; Hiv Infections; Lung Injury; Asthma; Cell Invasion; Systemic Infection|
|Related Pathways||Chemotaxis; Angiogenesis; Pathogenesis; Secretion; Inflammatory Response; Cell Migration; Neutrophil Chemotaxis; Cell Proliferation; Immune Response; Receptor Internalization; Cell Adhesion; Neutrophil Activation; Leukocyte Migration; Wound Healing; Chemokine Production; Localization; Tube Formation; Endocytosis; Cell Activation; Innate Immune Response|
|Background||CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family(subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.|
|Scientific References||PMID: 1891716 by Murphy P.M., et al. Cloning of complementary DNA encoding a functional human interleukin- 8 receptor.|
PMID: 8384312 by Cerretti D.P., et al. Molecular characterization of receptors for human interleukin-8, GRO/melanoma growth-stimulatory activity and neutrophil activating peptide-2.
Our Boster Quality Guarantee for Anti-CXCR2 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Human Rectal Cancer Tissue Lysate
Lane 2: Human Rectal Cancer Tissue Lysate
All lanes: Anti CXCR2(PA1029) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Lane 6: Mouse BrainTissue Lysate at 50ug
Predicted bind size: 41KD
Observed bind size: 41KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 5
|Zhang Zj, Cao Dl, Zhang X, Ji Rr, Gao Yj. Pain. 2013 Oct;154(10):2185-97. Doi: 10.1016/J.Pain.2013.07.002. Epub 2013 Jul 4. Chemokine Contribution To Neuropathic Pain: Respective Induction Of Cxcl1 And Cxcr2 In Spinal Cord Astrocytes And Neurons.||PubMed ID 23831863|
|Chen G, Park Ck, Xie Rg, Berta T, Nedergaard M, Ji Rr. Brain. 2014 Aug;137(Pt 8):2193-209. Doi: 10.1093/Brain/Awu140. Epub 2014 Jun 11. Connexin-43 Induces Chemokine Release From Spinal Cord Astrocytes To Maintain Late-Phase Neuropathic Pain In Mice.||PubMed ID 24919967|
|Xu J, Zhu Md, Zhang X, Tian H, Zhang Jh, Wu Xb, Gao Yj. J Neuroinflammation. 2014 Mar 1;11:38. Doi: 10.1186/1742-2094-11-38. Nf??b-Mediated Cxcl1 Production In Spinal Cord Astrocytes Contributes To The Maintenance Of Bone Cancer Pain In Mice.||PubMed ID 24580964|
|Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2||PubMed ID 24852102|
|Overexpression of GRK6 attenuates neuropathic pain via suppression of CXCR2 in rat dorsal root ganglion||PubMed ID 27145805|
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Note: sample size takes longer to process, average 5-7 days.
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