Click image to see more details
Product Info Summary
| SKU: | PA1760 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GRO alpha/CXCL1 Antibody Picoband®
SKU/Catalog Number
PA1760
BA1471-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GRO alpha/CXCL1 Antibody catalog # PA1760. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRO alpha/CXCL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1760)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GRO alpha.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1760 is reactive to CXCL1 in Human
Observed Molecular Weight
11 kDa
Calculated molecular weight
11.3 kDa
Background of CXCL1
CXCL1 (Chemokine, CXC motif, Ligand 1), also called GRO1, SCYB1, GROA or MGSA, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The CXCL1 gene is mapped on 4q13.3. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation,wound healing, and tumorigenesis. Signaling through Cxcr2, Cxcl1 inhibited oligodendrocyte precursor migration. The migrational arrest was rapid, reversible, and concentration dependent, and it reflected enhanced cell/substrate interactions. White matter expression of Cxcl1 was temporospatially regulated. Others contribute to aggressive growth selectivity in the lung. Among the lung metastasis signature genes identified, several, including CXCL1, were functionally validated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1760 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, JURKAT Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-GRO alpha antibody, PA1760, Western blotting
All lanes: Anti GRO alpha (PA1760) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
Specific Publications For Anti-GRO alpha/CXCL1 Antibody Picoband® (PA1760)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GRO alpha/CXCL1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-GRO alpha/CXCL1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
8 Customer Q&As for Anti-GRO alpha/CXCL1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-GRO alpha/CXCL1 antibody (PA1760)?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We do provide the blocking peptide for product anti-GRO alpha/CXCL1 antibody (PA1760). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-12
Question
Will anti-GRO alpha/CXCL1 antibody PA1760 work on feline WB with skin?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Our lab technicians have not validated anti-GRO alpha/CXCL1 antibody PA1760 on feline. You can run a BLAST between feline and the immunogen sequence of anti-GRO alpha/CXCL1 antibody PA1760 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-24
Question
Do you have a BSA free version of anti-GRO alpha/CXCL1 antibody PA1760 available?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GRO alpha/CXCL1 antibody PA1760 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-04
Question
Could I use this antibody to detect mouse samples? Keyword: applications, species sample type, protocol
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
This product can't be used to detect mouse samples.
Boster Scientific Support
Answered: 2018-12-14
Question
I would like to test anti-GRO alpha/CXCL1 antibody PA1760 on human blood for research purposes, then I may be interested in using anti-GRO alpha/CXCL1 antibody PA1760 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Taylor
Verified customer
Asked: 2018-01-09
Answer
The products we sell, including anti-GRO alpha/CXCL1 antibody PA1760, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-09
Question
We are currently using anti-GRO alpha/CXCL1 antibody PA1760 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
The anti-GRO alpha/CXCL1 antibody (PA1760) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-08
Question
I was wanting to use your anti-GRO alpha/CXCL1 antibody for WB for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
You can see on the product datasheet, PA1760 anti-GRO alpha/CXCL1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-07
Question
I see that the anti-GRO alpha/CXCL1 antibody PA1760 works with WB, what is the protocol used to produce the result images on the product page?
R. Kulkarni
Verified customer
Asked: 2014-04-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-04-03
