Anti-GRO alpha/CXCL1 Antibody


SKU PA1760
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-GRO alpha/CXCL1 Antibody
SKU/Catalog Number PA1760
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Growth-regulated alpha protein(CXCL1) detection. Tested with WB in Human.
Cite This Product Anti-GRO alpha/CXCL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1760)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human GRO alpha(99-107aa KMLNSDKSN).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

Anti-GRO alpha antibody, PA1760, Western blotting
All lanes: Anti GRO alpha (PA1760) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P09341
Gene Name CXCL1
Protein Name Growth-regulated alpha protein
Alternative Names Growth-regulated alpha protein;C-X-C motif chemokine 1;GRO-alpha(1-73);Melanoma growth stimulatory activity;MGSA;Neutrophil-activating protein 3;NAP-3;GRO-alpha(4-73);GRO-alpha(5-73);GRO-alpha(6-73);CXCL1;GRO, GRO1, GROA, MGSA, SCYB1;
Subcellular Localization Secreted.
Molecular Weight 11301 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. .
Research Areas Human

*You can search these to find other products in these research areas.
Background CXCL1(Chemokine, CXC motif, Ligand 1), also called GRO1, SCYB1, GROA or MGSA, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The CXCL1 gene is mapped on 4q13.3. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation,wound healing, and tumorigenesis. Signaling through Cxcr2, Cxcl1 inhibited oligodendrocyte precursor migration. The migrational arrest was rapid, reversible, and concentration dependent, and it reflected enhanced cell/substrate interactions. White matter expression of Cxcl1 was temporospatially regulated. Others contribute to aggressive growth selectivity in the lung. Among the lung metastasis signature genes identified, several, including CXCL1, were functionally validated.

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Polyclonal antibody for GRO alpha/CXCL1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. GRO alpha/CXCL1 information: Molecular Weight: 11301 MW; Subcellular Localization: Secreted.
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In stock
Order Product
PA1760
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Publications

Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2
Xu J, Zhu Md, Zhang X, Tian H, Zhang Jh, Wu Xb, Gao Yj. J Neuroinflammation. 2014 Mar 1;11:38. Doi: 10.1186/1742-2094-11-38. Nf??b-Mediated Cxcl1 Production In Spinal Cord Astrocytes Contributes To The Maintenance Of Bone Cancer Pain In Mice.
Chen G, Park Ck, Xie Rg, Berta T, Nedergaard M, Ji Rr. Brain. 2014 Aug;137(Pt 8):2193-209. Doi: 10.1093/Brain/Awu140. Epub 2014 Jun 11. Connexin-43 Induces Chemokine Release From Spinal Cord Astrocytes To Maintain Late-Phase Neuropathic Pain In Mice.
Zhang Zj, Cao Dl, Zhang X, Ji Rr, Gao Yj. Pain. 2013 Oct;154(10):2185-97. Doi: 10.1016/J.Pain.2013.07.002. Epub 2013 Jul 4. Chemokine Contribution To Neuropathic Pain: Respective Induction Of Cxcl1 And Cxcr2 In Spinal Cord Astrocytes And Neurons.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Could I use this antibody to detect mouse samples? Keyword: applications, species sample type, protocol
    A: This product can't be used to detect mouse samples.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to cxcl1 antibody, fsp antibody, kc antibody
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