|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-CXCR3 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 3(CXCR3) detection. Tested with WB, IHC-P in Human;Rat.|
|Cite This Product||Anti-CXCR3 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9079)|
|Replacement Item||This antibody may replace the following items: sc-133087|sc-133121|sc-137140|sc-13951|sc-6226|sc-9900|sc-9901 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human CXCR3 recombinant protein (Position: M1-L368). Human CXCR3 shares 86% amino acid (aa) sequence identity with both mouse and rat CXCR3.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||C-X-C chemokine receptor type 3|
|Molecular Weight||40660 MW|
|Protein Function||Isoform 1: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed:12782716). Binds to CCL21. Probably promotes cell chemotaxis response. .|
|Tissue Specificity||Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level). .|
|Sequence Similarities||Belongs to the G-protein coupled receptor 1 family.|
|Subcellular Localization||Isoform 1: Cell membrane ; Multi-pass membrane protein .|
|Alternative Names||C-X-C chemokine receptor type 3;CXC-R3;CXCR-3;CKR-L2;G protein-coupled receptor 9;Interferon-inducible protein 10 receptor;IP-10 receptor;CD183;CXCR3;GPR9;|
|Research Areas|||immunology|innate immunity|chemokines|alpha chemokine rec. (cxcr)| signal transduction|signaling pathway|calcium signaling|g protein signaling|gpcr| cardiovascular|atherosclerosis|vascular inflammation|leukocyte recruitment||
Background for C-X-C chemokine receptor type 3
Anti-CXCR3 Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Rat Kidney Tissue
IHC(P): Human Tonsil Tissue
IHC(P): Human Lung Cancer Tissue
All lanes: Anti-CXCR3(PB9079) at 0.5ug/ml
Lane 1: Colo320 Whole Cell Lysate at 40ug
Lane 2: SGC Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,