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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.|
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Human Rantes ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Rantes ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL5 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 3pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Rantes ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0494)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: S24-S91
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0494 is reactive to CCL5 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
EK0494 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL5/RANTES
Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
|EK0494-CAP||Anti-Human CCL5 Pre-coated 96-well strip microplate||1|
|EK0494-ST||Human CCL5 Standard||2 vials, 10 ng/tube|
|EK0494-DA||Human CCL5 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Rantes PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL5/RANTES, Dilution ratio of 1:100, concentration in serum is 30-50ng/ml.
Some articles we found to cite concentrations of CCL5/RANTES in samples: 17504241 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Rantes ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CCL5/RANTES in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CCL5 (Source: Uniprot.Org, NCBI)
C-C motif chemokine 5
intercrine beta (chemokine CC) family
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES CCL5 D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP C-C motif chemokine ligand 5 C-C motif chemokine 5|T-cell specific protein p288|beta-chemokine RANTES|chemokine (C-C motif) ligand 5|eosinophil chemotactic cytokine|regulated upon activation, normally T-expressed, and presumably secreted|small inducible cytokine subfamily A (Cys-Cys), member 5*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CCL5, check out the CCL5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0494 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
In vitro modeling of COPD inflammation and limitation of p38 inhibitor – SB203580
Mesenchymal stem cells in inflammatory microenvironment potently promote metastatic growth of cholangiocarcinoma via activating Akt/NF-κB signaling by paracrine CCL5
Combination of Gluten-Digesting Enzymes Improved Symptoms of Non-Celiac Gluten Sensitivity: A Randomized Single-blind, Placebo-controlled Crossover Study
“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model
Inflammation-Associated Cytokines IGFBP1 and RANTES Impair the Megakaryocytic Potential of HSCs in PT Patients after Allo-HSCT
Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310.
EK0494 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA
Chen X,Gao A,Zhang F,Yang Z,Wang S,Fang Y,Li J,Wang J,Shi W,Wang L, Zheng Y,Sun Y.ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.Theranostics.
EK0494 usage in article: APP:ELISA, SAMPLE:TUMOR CELLS, DILUTION:NA
Mesenchymal stem cells in inflammatory microenvironment potently promote metastatic growth of cholangiocarcinoma via activating Akt/NF-?B signaling by paracrine CCL5
Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway
Zhao C, Bu X, Wang W, Ma T, Ma H. Plos One. 2014 Jan 8;9(1):E85058. Doi: 10.1371/Journal.Pone.0085058. Ecollection 2014. Gec-Derived Sfrp5 Inhibits Wnt5A-Induced Macrophage Chemotaxis And Activation.
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