Anti-CXCR5 Antibody

CXCR5 antibody

Boster Bio Anti-CXCR5 Antibody catalog # PA2021. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA2021
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-CXCR5 Antibody

View all CXCR5 Antibodies

SKU/Catalog Number

PA2021

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CXCR5 Antibody catalog # PA2021. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CXCR5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2021)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CXCR5, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2021 is reactive to CXCR5 in Human, Mouse, Rat

Applications

PA2021 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

41.955kDa

Background of CXCR5

CXCR5 (Chemokine CXC Motif Receptor 5), also known as BLR1, is a G protein-coupled seven transmembrane receptor for chemokine CXCL13 (also known as BLC) and belongs to the CXC chemokine receptor family. In humans, the CXC-R5 protein is encoded by the CXCR5 gene. The gene plays an essential role in B cell migration. Reif et al. (2002) concluded that their findings defined the mechanism of B-cell relocalization in response to antigen, and established that cell position in vivo can be determined by the balance of responsiveness to chemoattractants made in separate but adjacent zones. Chan et al. (2003) investigated the expression of chemokines and chemokine receptors in eyes with primary intraocular B-cell lymphoma (PIOL).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For CXCR5 (Source: Uniprot.org, NCBI)

Gene Name

CXCR5

Full Name

C-X-C chemokine receptor type 5

Weight

41.955kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15 CXCR5 BLR1, CD185, MDR15 C-X-C motif chemokine receptor 5 C-X-C chemokine receptor type 5|Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5)|Burkitt lymphoma receptor 1, GTP-binding protein|CXC-R5|CXCR-5|MDR-15|chemokine (C-X-C motif) receptor 5|monocyte-derived receptor 15

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CXCR5, check out the CXCR5 Infographic

CXCR5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CXCR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-CXCR5 Antibody

Question

We have seen staining in rat leukocyte. Are there any suggestions? Is anti-CXCR5 antibody supposed to stain leukocyte positively?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

From literature leukocyte does express CXCR5. From Uniprot.org, CXCR5 is expressed in leukocyte, lymphocyte, blood, among other tissues. Regarding which tissues have CXCR5 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7639692
Lymphocyte, Pubmed ID: 1425907

Boster Scientific Support

Answered: 2020-01-17

Question

My team were happy with the WB result of your anti-CXCR5 antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CXCR5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

From literature, leukocyte does express CXCR5. Generally CXCR5 expresses in cell membrane. Regarding which tissues have CXCR5 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7639692
Lymphocyte, Pubmed ID: 1425907

Boster Scientific Support

Answered: 2019-10-14

Question

Would PA2021 anti-CXCR5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

E. Moore

Verified customer

Asked: 2019-10-07

Answer

You can see on the product datasheet, PA2021 anti-CXCR5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-07

Question

We are currently using anti-CXCR5 antibody PA2021 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

The anti-CXCR5 antibody (PA2021) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-22

Question

My question regarding product PA2021, anti-CXCR5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-01-29

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2021 anti-CXCR5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-29

Question

Will anti-CXCR5 antibody PA2021 work for WB with blood?

S. Anderson

Verified customer

Asked: 2018-11-26

Answer

According to the expression profile of blood, CXCR5 is highly expressed in blood. So, it is likely that anti-CXCR5 antibody PA2021 will work for WB with blood.

Boster Scientific Support

Answered: 2018-11-26

Question

Do you have a BSA free version of anti-CXCR5 antibody PA2021 available?

Verified Customer

Verified customer

Asked: 2018-05-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CXCR5 antibody PA2021 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-05-17

Question

I was wanting to use your anti-CXCR5 antibody for WB for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?

Verified Customer

Verified customer

Asked: 2017-10-17

Answer

As indicated on the product datasheet, PA2021 anti-CXCR5 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-17

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CXCR5 antibody PA2021. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-09-05

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-05

Question

We want to test anti-CXCR5 antibody PA2021 on human blood for research purposes, then I may be interested in using anti-CXCR5 antibody PA2021 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-29

Answer

The products we sell, including anti-CXCR5 antibody PA2021, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-29

Question

My question regards using your anti-CXCR5 antibody for g protein-coupled receptor signaling pathway studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-05-30

Answer

Thank you for your inquiry. This PA2021 anti-CXCR5 antibody is validated on rat spleen tissue, tissue lysate, hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-05-30

Question

Here is the WB image, lot number and protocol we used for blood using anti-CXCR5 antibody PA2021. Please let me know if you require anything else.

G. Krishna

Verified customer

Asked: 2017-03-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-03-16

Question

Is a blocking peptide available for product anti-CXCR5 antibody (PA2021)?

M. Parker

Verified customer

Asked: 2016-04-21

Answer

We do provide the blocking peptide for product anti-CXCR5 antibody (PA2021). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-04-21

Question

I see that the anti-CXCR5 antibody PA2021 works with WB, what is the protocol used to produce the result images on the product page?

D. Gonzalez

Verified customer

Asked: 2015-03-02

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-03-02

Question

Is this PA2021 anti-CXCR5 antibody reactive to the isotypes of CXCR5?

G. Singh

Verified customer

Asked: 2013-06-20

Answer

The immunogen of PA2021 anti-CXCR5 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CXCR5(358-372aa SSLSESENATSLTTF), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-06-20

Question

Does anti-CXCR5 antibody PA2021 work on primate WB with lymphocyte?

C. Johnson

Verified customer

Asked: 2013-02-21

Answer

Our lab technicians have not tested anti-CXCR5 antibody PA2021 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CXCR5 antibody PA2021 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lymphocyte in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-02-21

Order DetailsPrice
PA2021

100μg

$370
PA2021-10ug

10μg sample (liquid)

$99
PA2021-Biotin

100 μg Biotin conjugated

$570
PA2021-Cy3

100 μg Cy3 conjugated

$570
PA2021-Dylight488

100 μg Dylight488 conjugated

$570
PA2021-Dylight550

100 μg Dylight550 conjugated

$570
PA2021-Dylight594

100 μg Dylight594 conjugated

$570
PA2021-FITC

100 μg FITC conjugated

$570
PA2021-HRP

100 μg HRP conjugated

$570
PA2021-APC

100 μg APC conjugated

$670
PA2021-PE

100 μg PE conjugated

$670
PA2021-carrier-free

Carrier Free

$370

More conjugates/formats

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PA2021
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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