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Product Info Summary
| SKU: | PA2081 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CXCR4 Antibody Picoband®
SKU/Catalog Number
PA2081
BA0747-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCR4 Antibody catalog # PA2081. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCR4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2081)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2081 is reactive to CXCR4 in Human
Observed Molecular Weight
50 kDa
Calculated molecular weight
39.7 kDa
Background of CXCR4
CXCR4 (Chemokine,CXC Motif, Receptor 4), also known as FUSIN or NPY3R, is a protein that in humans is encoded by the CXCR4 gene. It is the receptor for the CXC chemokine SDF1 that has essential functions on embryo organogenesis, immunological functions and T lymphocyte trafficking. CXCR4 is the only SDF1 receptor identified so far. This suggests that CXCR4 expression is critical for the biological effects of SDF1. CXCR4 is also a seven-transmembrane-spanning, G-protein-coupled receptor for the CXC chemokine PBSF/SDF-1. It functions as a co-receptor for T-cell-line tropic human immunodeficiency virus HIV-1. It was concluded that PBSF/SDF-1 and CXCR4 define a new signalling system for organ vascularization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2081 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: M231 Cell, MCF-7 Cell, JURKAT Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-CXCR4 antibody, PA2081, Western blotting
Lane 1: M231 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: JURKAT Cell Lysate
Specific Publications For Anti-CXCR4 Antibody Picoband® (PA2081)
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Customer Q&As
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16 Customer Q&As for Anti-CXCR4 Antibody Picoband®
Question
Do you have a BSA free version of anti-CXCR4 antibody PA2081 available?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
I can confirm that some lots of this anti-CXCR4 antibody PA2081 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge.
Boster Scientific Support
Answered: 2020-05-08
Question
We have seen staining in human peripheral blood lymphocyte. Any tips? Is anti-CXCR4 antibody supposed to stain peripheral blood lymphocyte positively?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Based on literature peripheral blood lymphocyte does express CXCR4. Based on Uniprot.org, CXCR4 is expressed in oviduct epithelium, lung, fetal brain, fetal spleen, monocyte, peripheral blood leukocyte, peripheral blood lymphocyte, neutrophil, adrenal gland, colon, cervix carcinoma, among other tissues. Regarding which tissues have CXCR4 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231, 23186163
Colon, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 8234909
Fetal spleen, Pubmed ID: 8325644
Lung, Pubmed ID: 8329116, 15815621
Monocyte, Pubmed ID: 8276799
Boster Scientific Support
Answered: 2020-05-06
Question
Will anti-CXCR4 antibody PA2081 work on goat WB with oviduct epithelium?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Our lab technicians have not validated anti-CXCR4 antibody PA2081 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CXCR4 antibody PA2081 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat oviduct epithelium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for monocyte using anti-CXCR4 antibody PA2081. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-31
Question
Is this PA2081 anti-CXCR4 antibody reactive to the isotypes of CXCR4?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The immunogen of PA2081 anti-CXCR4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4(31-43aa EENANFNKIFLPT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-04
Question
I have attached the WB image, lot number and protocol we used for monocyte using anti-CXCR4 antibody PA2081. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-05
Question
My question regards using your anti-CXCR4 antibody for myelin maintenance studies. Has this antibody been tested with western blotting on m231 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
Thanks for your inquiry. This PA2081 anti-CXCR4 antibody is tested on m231 cell lysate, jurkat cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-03-01
Question
My team were satisfied with the WB result of your anti-CXCR4 antibody. However we have seen positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is CXCR4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
From what I have seen in literature, cervix carcinoma does express CXCR4. Generally CXCR4 expresses in cell membrane. Regarding which tissues have CXCR4 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231, 23186163
Colon, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 8234909
Fetal spleen, Pubmed ID: 8325644
Lung, Pubmed ID: 8329116, 15815621
Monocyte, Pubmed ID: 8276799
Boster Scientific Support
Answered: 2018-08-30
Question
My question regarding product PA2081, anti-CXCR4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2081 anti-CXCR4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-05
Question
Would anti-CXCR4 antibody PA2081 work for WB with monocyte?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
According to the expression profile of monocyte, CXCR4 is highly expressed in monocyte. So, it is likely that anti-CXCR4 antibody PA2081 will work for WB with monocyte.
Boster Scientific Support
Answered: 2018-01-11
Question
Is a blocking peptide available for product anti-CXCR4 antibody (PA2081)?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
We do provide the blocking peptide for product anti-CXCR4 antibody (PA2081). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-10-31
Question
I see that the anti-CXCR4 antibody PA2081 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-18
Question
Will PA2081 anti-CXCR4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Evans
Verified customer
Asked: 2016-07-15
Answer
As indicated on the product datasheet, PA2081 anti-CXCR4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-07-15
Question
We are currently using anti-CXCR4 antibody PA2081 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
B. Walker
Verified customer
Asked: 2016-07-07
Answer
The anti-CXCR4 antibody (PA2081) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-07
Question
We want to test anti-CXCR4 antibody PA2081 on human monocyte for research purposes, then I may be interested in using anti-CXCR4 antibody PA2081 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Roberts
Verified customer
Asked: 2016-01-14
Answer
The products we sell, including anti-CXCR4 antibody PA2081, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-01-14
Question
I was wanting to use your anti-CXCR4 antibody for WB for human monocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human monocyte identification?
N. Brown
Verified customer
Asked: 2014-10-07
Answer
It shows on the product datasheet, PA2081 anti-CXCR4 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human monocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-07
