Anti-CXCR4 Antibody

Boster Bio Anti-CXCR4 Antibody catalog # PA2081. Tested in WB applications. This antibody reacts with Human. Cited in 13 publication(s).

Product Info Summary

SKU: PA2081
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-CXCR4 Antibody

See all CXCR4 products

SKU/Catalog Number

PA2081

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CXCR4 Antibody catalog # PA2081. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CXCR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2081)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4 (31-43aa EENANFNKIFLPT).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA2081 is reactive to CXCR4 in Human

Applications

PA2081 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CXCR4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P61073

Gene ID

7852

Gene Name

CXCR4

Full Name

C-X-C chemokine receptor type 4

Weight

39.746kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS C-X-C motif chemokine receptor 4 C-X-C chemokine receptor type 4|CD184 antigen|LPS-associated protein 3|SDF-1 receptor|chemokine (C-X-C motif) receptor 4|fusin|leukocyte-derived seven transmembrane domain receptor|lipopolysaccharide-associated protein 3|neuropeptide Y receptor Y3|neuropeptide Y3 receptor|seven transmembrane helix receptor|seven-transmembrane-segment receptor, spleen|stromal cell-derived factor 1 receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCR4, check out the CXCR4 Infographic

CXCR4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCR4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA2081 has been cited in 13 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Macrophage migration inhibitory factor%u2013CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors

Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer

Blockade of CXCL12/CXCR4 signaling inhibits intrahepatic cholangiocarcinoma progression and metastasis via inactivation of canonical Wnt pathway

Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF-1? expression?in vivo

Wang J, Cai J, Han F, Yang C, Tong Q, Cao T, Wu L, Wang Z. Int J Gynecol Cancer. 2011 Aug;21(6):981-7. Doi: 10.1097/Igc.0B013E31821D2543. Silencing Of Cxcr4 Blocks Progression Of Ovarian Cancer And Depresses Canonical Wnt Signaling Pathway.

Li Y, Chen M, Yuan J, Zhou X, He W, Zu X, Qi L. Urol Int. 2014;92(2):157-63. Doi: 10.1159/000351952. Epub 2013 Aug 27. Cxcr4 Expression In Bladder Transitional Cell Carcinoma And Its Relationship With Clinicopathological Features.

Tan Xy, Chang S, Liu W, Tang Hh. Gut Liver. 2014 Mar;8(2):196-204. Doi: 10.5009/Gnl.2014.8.2.196. Epub 2013 Nov 5. Silencing Of Cxcr4 Inhibits Tumor Cell Proliferation And Neural Invasion In Human Hilar Cholangiocarcinoma.

Li S, Deng L, Gong L, Bian H, Dai Y, Wang Y. Neurosci Res. 2010 Aug;67(4):293-9. Doi: 10.1016/J.Neures.2010.04.006. Epub 2010 Apr 20. Upregulation Of Cxcr4 Favoring Neural-Like Cells Migration Via Akt Activation.

Lin F, Zheng Se, Shen Z, Tang Ln, Chen P, Sun Yj, Zhao H, Yao Y. Med Oncol. 2011 Jun;28(2):649-53. Doi: 10.1007/S12032-010-9493-4. Epub 2010 Mar 27. Relationships Between Levels Of Cxcr4 And Vegf And Blood-Borne Metastasis And Survival In Patients...

Chen Hy, Wang Jm, Wang Hy, Zhang Yx, Liu W, Pan L, Wang Wh, Chen Sf, Jin Wg, Wang L. Biomed Pharmacother. 2012 Oct;66(7):549-53. Doi: 10.1016/J.Biopha.2012.04.007. Epub 2012 May 24. Effect Of Short Hairpin Rna-Induced Cxcr4 Silence On Ovarian Canc...

Wang D, Guo S, Han Sy, Xu N, Guo Jy, Sun Q. Int J Oncol. 2013 May;42(5):1725-33. Doi: 10.3892/Ijo.2013.1841. Epub 2013 Feb 27. Distinct Roles Of Different Fragments Of Pdcd4 In Regulating The Metastatic Behavior Of B16 Melanoma Cells.

Zhang W, Wang X, Yue K, Liu S, Liu X. Biomed Res Int. 2015;2015:195828. Doi: 10.1155/2015/195828. Epub 2015 Mar 19. Construction Of A Cxcl12-Kdel Fusion Gene To Inhibit Head And Neck Squamous Cell Carcinoma Metastasis By Intracellular Sequestratio...

Liu W, Wang Y, Wang H, Wang A. Cell Biochem Biophys. 2014 Dec;70(3):1893-900. Doi: 10.1007/S12013-014-0148-5. Anticancer Effects Of Chemokine Receptor 4(Cxcr4) Gene Silenced By Cxcr4-Sirna In Nude Mice Model Of Ovarian Cancer.

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16 Customer Q&As for Anti-CXCR4 Antibody

Question

Do you have a BSA free version of anti-CXCR4 antibody PA2081 available?

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

I can confirm that some lots of this anti-CXCR4 antibody PA2081 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge.

Boster Scientific Support

Answered: 2020-05-08

Question

We have seen staining in human peripheral blood lymphocyte. Any tips? Is anti-CXCR4 antibody supposed to stain peripheral blood lymphocyte positively?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

Based on literature peripheral blood lymphocyte does express CXCR4. Based on Uniprot.org, CXCR4 is expressed in oviduct epithelium, lung, fetal brain, fetal spleen, monocyte, peripheral blood leukocyte, peripheral blood lymphocyte, neutrophil, adrenal gland, colon, cervix carcinoma, among other tissues. Regarding which tissues have CXCR4 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231, 23186163
Colon, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 8234909
Fetal spleen, Pubmed ID: 8325644
Lung, Pubmed ID: 8329116, 15815621
Monocyte, Pubmed ID: 8276799

Boster Scientific Support

Answered: 2020-05-06

Question

Will anti-CXCR4 antibody PA2081 work on goat WB with oviduct epithelium?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

Our lab technicians have not validated anti-CXCR4 antibody PA2081 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CXCR4 antibody PA2081 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat oviduct epithelium in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-20

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for monocyte using anti-CXCR4 antibody PA2081. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-31

Question

Is this PA2081 anti-CXCR4 antibody reactive to the isotypes of CXCR4?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

The immunogen of PA2081 anti-CXCR4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4(31-43aa EENANFNKIFLPT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-04

Question

I have attached the WB image, lot number and protocol we used for monocyte using anti-CXCR4 antibody PA2081. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-05

Question

My question regards using your anti-CXCR4 antibody for myelin maintenance studies. Has this antibody been tested with western blotting on m231 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

Thanks for your inquiry. This PA2081 anti-CXCR4 antibody is tested on m231 cell lysate, jurkat cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-03-01

Question

My team were satisfied with the WB result of your anti-CXCR4 antibody. However we have seen positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is CXCR4 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-08-30

Answer

From what I have seen in literature, cervix carcinoma does express CXCR4. Generally CXCR4 expresses in cell membrane. Regarding which tissues have CXCR4 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231, 23186163
Colon, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 8234909
Fetal spleen, Pubmed ID: 8325644
Lung, Pubmed ID: 8329116, 15815621
Monocyte, Pubmed ID: 8276799

Boster Scientific Support

Answered: 2018-08-30

Question

My question regarding product PA2081, anti-CXCR4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-04-05

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2081 anti-CXCR4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-04-05

Question

Would anti-CXCR4 antibody PA2081 work for WB with monocyte?

Verified Customer

Verified customer

Asked: 2018-01-11

Answer

According to the expression profile of monocyte, CXCR4 is highly expressed in monocyte. So, it is likely that anti-CXCR4 antibody PA2081 will work for WB with monocyte.

Boster Scientific Support

Answered: 2018-01-11

Question

Is a blocking peptide available for product anti-CXCR4 antibody (PA2081)?

Verified Customer

Verified customer

Asked: 2017-10-31

Answer

We do provide the blocking peptide for product anti-CXCR4 antibody (PA2081). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-10-31

Question

I see that the anti-CXCR4 antibody PA2081 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-07-18

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-07-18

Question

Will PA2081 anti-CXCR4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Evans

Verified customer

Asked: 2016-07-15

Answer

As indicated on the product datasheet, PA2081 anti-CXCR4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-07-15

Question

We are currently using anti-CXCR4 antibody PA2081 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?

B. Walker

Verified customer

Asked: 2016-07-07

Answer

The anti-CXCR4 antibody (PA2081) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-07-07

Question

We want to test anti-CXCR4 antibody PA2081 on human monocyte for research purposes, then I may be interested in using anti-CXCR4 antibody PA2081 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Roberts

Verified customer

Asked: 2016-01-14

Answer

The products we sell, including anti-CXCR4 antibody PA2081, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-01-14

Question

I was wanting to use your anti-CXCR4 antibody for WB for human monocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human monocyte identification?

N. Brown

Verified customer

Asked: 2014-10-07

Answer

It shows on the product datasheet, PA2081 anti-CXCR4 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human monocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-10-07

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PA2081
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