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Pack Size:100μg/vial
Validated Species:Human
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Product Name Anti-CXCR4 Antibody
SKU/Catalog Number PA2081
Description Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 4(CXCR4) detection. Tested with WB in Human.
Cite This Product Anti-CXCR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2081)
Replacement Item This antibody may replace the following items: sc-12764|sc-366006|sc-376270|sc-398601|sc-53534|sc-6190|sc-6191|sc-6279|sc-82958|sc-82959|sc-82960|sc-9046 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4(31-43aa EENANFNKIFLPT).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CXCR4
Protein Name C-X-C chemokine receptor type 4
Molecular Weight 39746 MW
Protein Function Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. .
Tissue Specificity Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. .
Sequence Similarities Belongs to the G-protein coupled receptor 1 family.
Subcellular Localization Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC).
Uniprot ID P61073
Alternative Names C-X-C chemokine receptor type 4;CXC-R4;CXCR-4;FB22;Fusin;HM89;LCR1;Leukocyte-derived seven transmembrane domain receptor;LESTR;Lipopolysaccharide-associated protein 3;LAP-3;LPS-associated protein 3;NPYRL;Stromal cell-derived factor 1 receptor;SDF-1 receptor;CD184;CXCR4;
Research Areas |immunology|innate immunity|chemokines|alpha chemokine rec. (cxcr)| microbiology|interspecies interaction|host virus interaction| neuroscience|neurology process|growth and development|axonal guidance proteins| stem cells|neural stem cells|surface molecules|hematopoietic progenitors| immunology|adaptive immunity|regulatory t cells|endothelial progenitors|endothelial markers| cancer|cancer metabolism|response to hypoxia|immune system diseases|antiviral signaling|hiv-related| metabolism|pathways and processes|metabolism processes|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for C-X-C chemokine receptor type 4

CXCR4 (Chemokine,CXC Motif, Receptor 4), also known as FUSIN or NPY3R, is a protein that in humans is encoded by the CXCR4 gene. It is the receptor for the CXC chemokine SDF1 that has essential functions on embryo organogenesis, immunological functions and T lymphocyte trafficking. CXCR4 is the only SDF1 receptor identified so far. This suggests that CXCR4 expression is critical for the biological effects of SDF1. CXCR4 is also a seven-transmembrane-spanning, G-protein-coupled receptor for the CXC chemokine PBSF/SDF-1. It functions as a co-receptor for T-cell-line tropic human immunodeficiency virus HIV-1. It was concluded that PBSF/SDF-1 and CXCR4 define a new signalling system for organ vascularization.

Anti-CXCR4 Antibody Images

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Anti-CXCR4 Antibody
Anti-CXCR4 antibody, PA2081, Western blotting
Lane 1: M231 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: JURKAT Cell Lysate
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Wang J, Cai J, Han F, Yang C, Tong Q, Cao T, Wu L, Wang Z. Int J Gynecol Cancer. 2011 Aug;21(6):981-7. Doi: 10.1097/Igc.0B013E31821D2543. Silencing Of Cxcr4 Blocks Progression Of Ovarian Cancer And Depresses Canonical Wnt Signaling Pathway.
Li Y, Chen M, Yuan J, Zhou X, He W, Zu X, Qi L. Urol Int. 2014;92(2):157-63. Doi: 10.1159/000351952. Epub 2013 Aug 27. Cxcr4 Expression In Bladder Transitional Cell Carcinoma And Its Relationship With Clinicopathological Features.
Tan Xy, Chang S, Liu W, Tang Hh. Gut Liver. 2014 Mar;8(2):196-204. Doi: 10.5009/Gnl.2014.8.2.196. Epub 2013 Nov 5. Silencing Of Cxcr4 Inhibits Tumor Cell Proliferation And Neural Invasion In Human Hilar Cholangiocarcinoma.
Li S, Deng L, Gong L, Bian H, Dai Y, Wang Y. Neurosci Res. 2010 Aug;67(4):293-9. Doi: 10.1016/J.Neures.2010.04.006. Epub 2010 Apr 20. Upregulation Of Cxcr4 Favoring Neural-Like Cells Migration Via Akt Activation.
Lin F, Zheng Se, Shen Z, Tang Ln, Chen P, Sun Yj, Zhao H, Yao Y. Med Oncol. 2011 Jun;28(2):649-53. Doi: 10.1007/S12032-010-9493-4. Epub 2010 Mar 27. Relationships Between Levels Of Cxcr4 And Vegf And Blood-Borne Metastasis And Survival In Patients...
Chen Hy, Wang Jm, Wang Hy, Zhang Yx, Liu W, Pan L, Wang Wh, Chen Sf, Jin Wg, Wang L. Biomed Pharmacother. 2012 Oct;66(7):549-53. Doi: 10.1016/J.Biopha.2012.04.007. Epub 2012 May 24. Effect Of Short Hairpin Rna-Induced Cxcr4 Silence On Ovarian Canc...
Wang D, Guo S, Han Sy, Xu N, Guo Jy, Sun Q. Int J Oncol. 2013 May;42(5):1725-33. Doi: 10.3892/Ijo.2013.1841. Epub 2013 Feb 27. Distinct Roles Of Different Fragments Of Pdcd4 In Regulating The Metastatic Behavior Of B16 Melanoma Cells.
Zhang W, Wang X, Yue K, Liu S, Liu X. Biomed Res Int. 2015;2015:195828. Doi: 10.1155/2015/195828. Epub 2015 Mar 19. Construction Of A Cxcl12-Kdel Fusion Gene To Inhibit Head And Neck Squamous Cell Carcinoma Metastasis By Intracellular Sequestratio...
Liu W, Wang Y, Wang H, Wang A. Cell Biochem Biophys. 2014 Dec;70(3):1893-900. Doi: 10.1007/S12013-014-0148-5. Anticancer Effects Of Chemokine Receptor 4(Cxcr4) Gene Silenced By Cxcr4-Sirna In Nude Mice Model Of Ovarian Cancer.

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cxcr4 antibody, cd184 antibody, cxcr-4 antibody, lap3 antibody