Anti-JAM A pY280 F11R Antibody

Product Name

Anti-JAM A pY280 F11R Antibody

View all F11R Antibodies

SKU/Catalog Number

A02068

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-JAM A pY280 F11R Antibody (Catalog # A02068). Tested in ELISA, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. ?Aliquot contents and freeze at -20°C or below for extended storage. ?Avoid cycles of freezing and thawing. ?Centrifuge product if not completely clear after standing at room temperature. ?This product is stable for several weeks at 4°C as an undiluted liquid. ?Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-JAM A pY280 F11R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02068)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Affinity purified Anti-JAM A pY280 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-term and phosphorylated at the tyrosine 280 position of Human JAM A protein.

Reactive Species

A02068 is reactive to F11R in Human

Observed Molecular Weight

42 kDa

Background of F11R

Anti-JAM A pY280 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). JAM A pY280 Antibody detects junctional adhesion molecule A (JAM A) protein phosphorylated at the tyrosine 280 location. JAM A is involved in cell-cell contact and tight junction formation in epithelial cells. JAM A protein is a transmembrane protein expressed in epithelial and endothelial cells as well as in hematopoietic cells and is important for a variety of cellular processes including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. In addition, JAM A may be a potential therapeutic target for breast cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02068 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 5 ug/ml
IF Microscopy: User optimized
WB: 1 ug/ml
This affinity purified antibody has been tested for use in ELISA, IF, and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 32.5 kDa in size corresponding to JAM A by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information F11R

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